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Endothelial Transcriptomic Analysis
2015Endothelial cell activity can be studied targeting the mature cells within the tissues and blood vessels or it could be done using the precursor cells (EPC). For in vitro studies, pure EPC can be harvested from umbilical cord, peripheral blood and bone marrow.
Sharma, Dileep +3 more
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Group A Streptococcus Transcriptome Analysis
2020Global gene expression analyses in bacteria have undergone a dramatic transformation. Prior to the development of high-throughput sequencing technologies, real-time PCR or microarray studies were the mainstay of assessing differences in gene expression in bacteria. Real-time PCR remains a critical tool for targeted gene expression analyses.
Misú, Sanson, Anthony R, Flores
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Transcriptome Analysis Predicts Treatment Response
Cancer Discovery, 2021Abstract Researchers used transcriptome analysis to predict patients' responses to treatments with 80% accuracy on average. The procedure relies on identifying pairs of interacting genes that are lethal to cells when both are inactivated or that enable tumors to evade treatments.
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Novel algorithm for transcriptome analysis
Physiological Genomics, 2006A growing body of evidence implicates the oocyte as a key regulator of ovarian folliculogenesis and early embryonic development. We have screened bovine cDNA microarrays (containing expressed sequence tags representing >15,000 unique genes) with Cy3- and Cy5-labeled cDNA derived from bovine oocyte samples collected at two different stages of ...
Peter M, Saama +4 more
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Microscale transcriptome analysis
2018In the previous chapter, we have analysed some important applications of RNA-seq in the neurosciences. A common feature of the described papers is that the spatial resolution of the analysed transcriptome corresponds to a brain subregion (e.g., cerebellum, cortex, hippocampus, and so on).
Alessandro Cellerino, Michele Sanguanini
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Transcriptome analysis of equine sarcoids
Veterinary and Comparative Oncology, 2016AbstractEquine sarcoids are the most commonly detected skin tumours in Equidae. In the present research, a comparative transcriptomic analysis was performed which aimed at looking inside a tumour biology and identification of the expression profile as a potential source of cancer specific genes useful as biomarkers.
E, Semik +6 more
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Mesoscale transcriptome analysis
2018In the current chapter, we will discuss some publications that applied the previously described data analysis methods to genome-scale expression datasets in order to investigate aspects of brain organisation at the mesoscale level (i.e. at the level of areas and their connections).
Alessandro Cellerino, Michele Sanguanini
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Transcriptome analysis in heart failure
Current Opinion in Cardiology, 2016Genome-wide analysis of RNA abundances (transcriptome analysis) offers great potential to identify biomarkers of disease and to gain insight into mechanisms underlying the development of heart failure. I will discuss key factors in generating and evaluating transcriptome data, and recent studies that have contributed to the understanding of cardiac ...
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Transcriptome Assembly and Alternative Splicing Analysis
2014Alternative Splicing (AS) is the molecular phenomenon whereby multiple transcripts are produced from the same gene locus. As a consequence, it is responsible for the expansion of eukaryotic transcriptomes. Aberrant AS is involved in the onset and progression of several human diseases.
BONIZZONI, PAOLA +5 more
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