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Allergic Inflammation Triggers the Unfolded Protein Response and <i>Ormdl3</i> Expression in Rat Adipocytes, While miR-665 Inhibition Selectively Modulates the IRE1/XBP1 Pathway and IL-6 Secretion. [PDF]
Int J Mol SciNowakowska-Lewicka J +8 more
europepmc +1 more source
LBPs NPs suppress breast cancer progression by inhibiting YAP1 expression to induce ferroptosis and alter energy metabolism. [PDF]
Sci RepZhao F +8 more
europepmc +1 more source
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Materials for Improving Immune Cell Transfection
Advanced Materials, 2021Chimeric antigen receptor T cell (CAR‐T) therapy holds great promise for preventing and treating deadly diseases such as cancer. However, it remains challenging to transfect and engineer primary immune cells for clinical cell manufacturing.
Arun R K Kumar, Yufeng Shou, Brian Chan
exaly +2 more sources
Malaria transfection and transfection vectors
Trends in Parasitology, 2003Malaria remains a leading cause of death due to infectious disease. The completion of the Plasmodium falciparum genome sequencing project and release of preliminary proteomics data have significantly increased our understanding of the biology of this organism.
Gardiner, Donald L. +3 more
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Cold Spring Harbor Protocols, 2018
Strategies for the delivery of genes into eukaryotic cells fall into three categories: transfection by biochemical methods, transfection by physical methods, and virus-mediated transduction. “Optical transfection”—a physical transfection method—exploits the ability of light to create small transient pores in the plasma membrane of mammalian cells.
Priti, Kumar +2 more
openaire +2 more sources
Strategies for the delivery of genes into eukaryotic cells fall into three categories: transfection by biochemical methods, transfection by physical methods, and virus-mediated transduction. “Optical transfection”—a physical transfection method—exploits the ability of light to create small transient pores in the plasma membrane of mammalian cells.
Priti, Kumar +2 more
openaire +2 more sources
Transfection by Electroporation
Current Protocols in Molecular Biology, 2010AbstractElectroporation—the use of high‐voltage electric shocks to introduce DNA into cells—can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
Huntington, Potter, Richard, Heller
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2011
Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. As a nonviral delivery system, transfection has gained popularity. The cost-effective and rapid production of recombinant human therapeutic proteins in sufficient quantities has been one of the challenges faced in biopharmaceutical ...
Chahal, P., Durocher, Y., Kamen, A.
openaire +1 more source
Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. As a nonviral delivery system, transfection has gained popularity. The cost-effective and rapid production of recombinant human therapeutic proteins in sufficient quantities has been one of the challenges faced in biopharmaceutical ...
Chahal, P., Durocher, Y., Kamen, A.
openaire +1 more source