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PEGylation Enhances Colloidal Stability and Promotes Ligand-Mediated Targeting of LAF-Xenopeptide mRNA Complexes. [PDF]
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Malaria transfection and transfection vectors
Trends in Parasitology, 2003Malaria remains a leading cause of death due to infectious disease. The completion of the Plasmodium falciparum genome sequencing project and release of preliminary proteomics data have significantly increased our understanding of the biology of this organism.
Gardiner, Donald L. +3 more
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Cold Spring Harbor Protocols, 2018
Strategies for the delivery of genes into eukaryotic cells fall into three categories: transfection by biochemical methods, transfection by physical methods, and virus-mediated transduction. “Optical transfection”—a physical transfection method—exploits the ability of light to create small transient pores in the plasma membrane of mammalian cells.
Priti, Kumar +2 more
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Strategies for the delivery of genes into eukaryotic cells fall into three categories: transfection by biochemical methods, transfection by physical methods, and virus-mediated transduction. “Optical transfection”—a physical transfection method—exploits the ability of light to create small transient pores in the plasma membrane of mammalian cells.
Priti, Kumar +2 more
openaire +2 more sources
Transfection by Electroporation
Current Protocols in Molecular Biology, 2010AbstractElectroporation—the use of high‐voltage electric shocks to introduce DNA into cells—can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
Huntington, Potter, Richard, Heller
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2011
Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. As a nonviral delivery system, transfection has gained popularity. The cost-effective and rapid production of recombinant human therapeutic proteins in sufficient quantities has been one of the challenges faced in biopharmaceutical ...
Chahal, P., Durocher, Y., Kamen, A.
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Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. As a nonviral delivery system, transfection has gained popularity. The cost-effective and rapid production of recombinant human therapeutic proteins in sufficient quantities has been one of the challenges faced in biopharmaceutical ...
Chahal, P., Durocher, Y., Kamen, A.
openaire +1 more source
2003
Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of ...
Paul C, Bridgman +2 more
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Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of ...
Paul C, Bridgman +2 more
openaire +2 more sources
2018
Transfection is defined as the transfer of foreign nucleic acids into cells. In general, transfection may achieve either overexpression of a gene by the transfer of plasmid DNA or suppression of gene expression by RNA interference after transfer of small interfering RNA.
Andrea-Anneliese, Keller +4 more
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Transfection is defined as the transfer of foreign nucleic acids into cells. In general, transfection may achieve either overexpression of a gene by the transfer of plasmid DNA or suppression of gene expression by RNA interference after transfer of small interfering RNA.
Andrea-Anneliese, Keller +4 more
openaire +2 more sources
2019
Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. Transduction is another popular transfection technology that uses viral vectors to deliver foreign genes. In the past decade, transfection technologies have evolved tremendously, for stable clone generation, therapeutic protein ...
Do Minh, A. +3 more
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Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. Transduction is another popular transfection technology that uses viral vectors to deliver foreign genes. In the past decade, transfection technologies have evolved tremendously, for stable clone generation, therapeutic protein ...
Do Minh, A. +3 more
openaire +2 more sources