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Horizontal gene transfer (HGT) promotes the spread of genes within bacterial communities. Among the HGT mechanisms, natural transformation stands out as being encoded by the bacterial core genome.
Gabriel Carvalho+7 more
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The Transformation Experiment of Frederick Griffith II: Inclusion of Cellular Heredity for the Creation of Novel Microorganisms. [PDF]
So far, synthetic biology approaches for the construction of artificial microorganisms have fostered the transformation of acceptor cells with genomes from donor cells. However, this strategy seems to be limited to closely related bacterial species only,
Müller GA.
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The transformation of Cryptococcus spp. by Agrobacterium tumefaciens has proven to be a useful genetic tool. A number of factors affect transformation frequency. These factors include acetosyringone concentration, bacterial cell to yeast cell ratio, cell
Jianmin Fu+2 more
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The recurring growth of bacterium in newly developed resistant cells and a minimal level of bacterial infection rate are the main limiting factors of Agrobacterium-mediated transformation experiments in Hevea brasiliensis.
Jinu Udayabhanu+5 more
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Bacterial transformations of naphthothiophenes [PDF]
Vol. 63, no. 9, p. 3468, Table 1, subheading under heading "Total recovery": "((delta)mol)" should read "((mu)mol)." [This corrects the article on p. 3463 in vol. 63.].
Jan T. Andersson+2 more
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Mechanisms of Transforming DNA Uptake to the Periplasm of Bacillus subtilis
Transformation is a widely distributed mechanism of bacterial horizontal gene transfer that plays a role in the spread of antibiotic resistance and virulence genes and more generally in evolution.
Jeanette Hahn+2 more
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A novel method for DNA delivery into bacteria using cationic copolymers [PDF]
Amphiphilic copolymers have a wide variety of medical and biotechnological applications, including DNA transfection in eukaryotic cells. Still, no polymer-primed transfection of prokaryotic cells has been described.
V.V. de Souza+4 more
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Altering under-represented DNA sequences elevates bacterial transformation efficiency
A cornerstone of bacterial molecular biology is the ability to genetically manipulate the microbe under study. Many bacteria are difficult to manipulate genetically, a phenotype due in part to robust removal of newly acquired DNA, for example, by ...
Shuai Hu+5 more
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Natural transformation, or the uptake of naked DNA from the external milieu by bacteria, holds a unique place in the history of biology. This is both the beginning of the realization of the correct chemical nature of genes and the first technical step to
Vui Yin Seow+6 more
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Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis is a rapid and reliable method for bacterial identification.
Jin Ling+13 more
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