Results 1 to 10 of about 3,602,698 (355)

Bacterial Transformation Buffers Environmental Fluctuations through the Reversible Integration of Mobile Genetic Elements

open access: yesmBio, 2020
Horizontal gene transfer (HGT) promotes the spread of genes within bacterial communities. Among the HGT mechanisms, natural transformation stands out as being encoded by the bacterial core genome.
Gabriel Carvalho   +7 more
doaj   +2 more sources

The Transformation Experiment of Frederick Griffith II: Inclusion of Cellular Heredity for the Creation of Novel Microorganisms. [PDF]

open access: yesBioengineering (Basel)
So far, synthetic biology approaches for the construction of artificial microorganisms have fostered the transformation of acceptor cells with genomes from donor cells. However, this strategy seems to be limited to closely related bacterial species only,
Müller GA.
europepmc   +2 more sources

Optimizing Transformation Frequency of Cryptococcus neoformans and Cryptococcus gattii Using Agrobacterium tumefaciens

open access: yesJournal of Fungi, 2021
The transformation of Cryptococcus spp. by Agrobacterium tumefaciens has proven to be a useful genetic tool. A number of factors affect transformation frequency. These factors include acetosyringone concentration, bacterial cell to yeast cell ratio, cell
Jianmin Fu   +2 more
doaj   +1 more source

Optimization of the Transformation Protocol for Increased Efficiency of Genetic Transformation in Hevea brasiliensis

open access: yesPlants, 2022
The recurring growth of bacterium in newly developed resistant cells and a minimal level of bacterial infection rate are the main limiting factors of Agrobacterium-mediated transformation experiments in Hevea brasiliensis.
Jinu Udayabhanu   +5 more
doaj   +1 more source

Bacterial transformations of naphthothiophenes [PDF]

open access: yesApplied and Environmental Microbiology, 1997
Vol. 63, no. 9, p. 3468, Table 1, subheading under heading "Total recovery": "((delta)mol)" should read "((mu)mol)." [This corrects the article on p. 3463 in vol. 63.].
Jan T. Andersson   +2 more
openaire   +5 more sources

Mechanisms of Transforming DNA Uptake to the Periplasm of Bacillus subtilis

open access: yesmBio, 2021
Transformation is a widely distributed mechanism of bacterial horizontal gene transfer that plays a role in the spread of antibiotic resistance and virulence genes and more generally in evolution.
Jeanette Hahn   +2 more
doaj   +1 more source

A novel method for DNA delivery into bacteria using cationic copolymers [PDF]

open access: yesBrazilian Journal of Medical and Biological Research, 2021
Amphiphilic copolymers have a wide variety of medical and biotechnological applications, including DNA transfection in eukaryotic cells. Still, no polymer-primed transfection of prokaryotic cells has been described.
V.V. de Souza   +4 more
doaj   +1 more source

Altering under-represented DNA sequences elevates bacterial transformation efficiency

open access: yesmBio, 2023
A cornerstone of bacterial molecular biology is the ability to genetically manipulate the microbe under study. Many bacteria are difficult to manipulate genetically, a phenotype due in part to robust removal of newly acquired DNA, for example, by ...
Shuai Hu   +5 more
doaj   +1 more source

Multisite transformation in Neisseria gonorrhoeae: insights on transformations mechanisms and new genetic modification protocols

open access: yesFrontiers in Microbiology, 2023
Natural transformation, or the uptake of naked DNA from the external milieu by bacteria, holds a unique place in the history of biology. This is both the beginning of the realization of the correct chemical nature of genes and the first technical step to
Vui Yin Seow   +6 more
doaj   +1 more source

Helix Matrix Transformation Combined With Convolutional Neural Network Algorithm for Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Bacterial Identification

open access: yesFrontiers in Microbiology, 2020
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis is a rapid and reliable method for bacterial identification.
Jin Ling   +13 more
doaj   +1 more source

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