Results 61 to 70 of about 118,415 (245)

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source

Stability of metastable phases: pathway-dependent transformation kinetics of the γ phase in eutectic AuGe

Materials & Design
The transformation kinetics of metastable phases into more stable phases with simultaneous variation of the phase composition is studied in metallic systems using the binary Au72Ge28 eutectic alloy as model system.
Jürgen E.K. Schawe, Štefan T. Stanko, Marc-Georg Willinger, Jörg F. Löffler   +3 more
doaj   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

Effect of Microstructure on Stress Dependence of Transformation Induced Plasticity in TRIP800 Low-alloy Multiphase Steels

Iranian Journal of Materials Science and Engineering, 2020
An experimental–numerical methodology was used in order to study the microstructural effects on stress state dependency of martensitic transformation kinetics in two different TRIP800 low alloy multiphase steels.
F. Hosseinabadi, A. Rezaee-Bazzaz, M. Mazinani, B. Mohammad Sadeghi   +3 more
doaj  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Comparative study on the effects of Nb and V on the nucleation and growth of pearlite in high-carbon steels using in-situ method

Journal of Materials Research and Technology
The formation of pearlite in three high-carbon steels with different Nb and V additions was directly observed using a high-temperature laser scanning confocal microscope. The number densities and growth rates of pearlite nodules, and the overall pearlite
Keduo Yu, Mingxing Zhou, Min Zhu, Houxin Wang, Zhenye Chen, Weifan Yin, Xue Su, Junyu Tian, Guang Xu   +8 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Effect of prior martensite on bainitic transformation of high Si hypereutectoid steel

Journal of Materials Research and Technology
The paper discusses the effect of prior martensite on the bainitic transformation of a high Si hypereutectoid steel. The research uses a combination of experimental techniques, high-resolution dilatometry and X-ray diffraction analysis, and theoretical ...
Carlos Garcia-Mateo, Thomas Sourmail, Amandine Philippot, Lucia Morales-Rivas, Jose A. Jimenez   +4 more
doaj   +1 more source

The Effect of Initial Structure on Phase Transformation in Continuous Heating of a TA15 Titanium Alloy

Metals, 2017
The effect of initial structure on phase evolution in continuous heating of a near-α TA15 titanium alloy (Ti-6Al-2Zr-1Mo-1V) was experimentally investigated. To this end; three microstructures were obtained by multiple heat treatment: I-bimodal structure
Xiaoguang Fan, Qi Li, Anming Zhao, Yuguo Shi, Wenjia Mei   +4 more
doaj   +1 more source