Results 11 to 20 of about 50,997 (182)

The In Vitro Transgenic Rodent Assay in Primary MutaMouse Hepatocytes Compared to the Mammalian Cell Gene Mutation Assay Using the HPRT Gene

Environmental and Molecular Mutagenesis
ABSTRACT Gene mutations can be detected in mammalian cells in vitro using indicator genes such as the hypoxanthine‐guanine‐phosphoribosyltransferase (HPRT) gene. These assays have been adopted as OECD test guidelines (TG, e.g., OECD TG no. 476) and are used for regulatory purposes.
Alina Goepfert, Kylee Ronnenberg, Claudia Ruelker, Silke Spang, Naveed Honarvar, Robert Landsiedel   +5 more
openaire   +2 more sources

A PERIOD3 variant causes a circadian phenotype and is associated with a seasonal mood trait. [PDF]

, 2016
In humans, the connection between sleep and mood has long been recognized, although direct molecular evidence is lacking. We identified two rare variants in the circadian clock gene PERIOD3 (PER3-P415A/H417R) in humans with familial advanced sleep phase ...
Fu, Ying-Hui, Hirano, Arisa, Hsu, Pei-Ken, Jones, Christopher R, Kaasik, Krista, Lin, Shu-Ting, McMahon, Thomas, Nishino, Seiji, Okuro, Masashi, Ptáček, Louis J, Saigoh, Kazumasa, Saigoh, Noriko, Sakai, Noriaki, Xu, Ying, Yamazaki, Maya, Zhang, Luoying   +15 more
core   +1 more source

High titers of transmissible spongiform encephalopathy infectivity associated with extremely low levels of PrP in vivo [PDF]

, 2007
Rona Barron - ORCID: 0000-0003-4512-9177 https://orcid.org/0000-0003-4512-9177Diagnosis of transmissible spongiform encephalopathy (TSE) disease in humans and ruminants relies on the detection in post-mortem brain tissue of the protease-resistant form of
Barron, R. M., Bellon, A., Campbell, S. L., Chapman, K. E., King, Declan, Manson, J., Williamson, R. A.   +6 more
core   +1 more source

EURL ECVAM strategy to avoid and reduce animal use in genotoxicity testing [PDF]

, 2013
The assessment of genotoxicity represents an important component of the safety assessment of all types of substances. Although several in vitro tests are available at different stages of development and acceptance, they cannot at present be considered to
CORVI Raffaella, MADIA FEDERICA, WHELAN Maurice, WORTH Andrew   +3 more
core   +1 more source

Interleukin‐18 signaling promotes activation of hepatic stellate cells in mouse liver fibrosis

Hepatology, EarlyView., 2022
Interleukin‐18 signaling promotes activation of hepatic stellate cells in mouse liver fibrosis. Abstract Background and Aims Nucleotide‐binding oligomerization domain‐like receptor‐family pyrin domain‐containing 3 (NLRP3) inflammasome activation has been shown to result in liver fibrosis.
Jana Knorr, Benedikt Kaufmann, Maria Eugenia Inzaugarat, Theresa Maria Holtmann, Lukas Geisler, Jana Hundertmark, Marlene Sophia Kohlhepp, Laela M. Boosheri, Daisy R. Chilin‐Fuentes, Amanda Birmingham, Kathleen M. Fisch, Joel D. Schilling, Sven H. Loosen, Christian Trautwein, Christoph Roderburg, Münevver Demir, Frank Tacke, Hal M. Hoffman, Ariel E. Feldstein, Alexander Wree   +19 more
wiley   +1 more source

Summarized data of genotoxicity tests for designated food additives in Japan

Genes and Environment, 2018
The Ministry of Health, Labour and Welfare has carried out genotoxicity tests for food additives used in Japan in cooperation with the Japan Food Additives Association since 1979. Hayashi et al.
Masami Yamada, Masamitsu Honma
doaj   +1 more source

Identifying germ cell mutagens using OECD test guideline 488 (transgenic rodent somatic and germ cell gene mutation assays) and integration with somatic cell testing

Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2018
The Organisation for Economic Co-operation and Development Test Guideline 488 (TG 488) provides recommendations for assessing germ cell and somatic cell mutagenicity using transgenic rodent (TGR) models. However, important data gaps exist for selecting an optimal approach for simultaneously evaluating mutagenicity in both cell types.
Francesco Marchetti, Marilyn J. Aardema, Carol Beevers, Jan van Benthem, Roger Godschalk, Andrew Williams, Carole L. Yauk, Robert Young, George R. Douglas   +8 more
openaire   +4 more sources

A novel, resistance-linked ovine PrP variant and its equivalent mouse variant modulate the in vitro cell-free conversion of rPrP to PrPres [PDF]

, 2006
Prion diseases are associated with the conversion of the normal cellular prion protein, PrPc, to the abnormal, disease-associated form, PrPSc. This conversion can be mimicked in vitro by using a cell-free conversion assay. It has recently been shown that
Gill, Andrew C., Goldmann, Wilfred, Houston, Fiona, Kirby, Louise, Manson, Jean C.   +4 more
core   +1 more source

Expansion of the Parkinson disease-associated SNCA-Rep1 allele upregulates human alpha-synuclein in transgenic mouse brain. [PDF]

, 2009
Alpha-synuclein (SNCA) gene has been implicated in the development of rare forms of familial Parkinson disease (PD). Recently, it was shown that an increase in SNCA copy numbers leads to elevated levels of wild-type SNCA-mRNA and protein and is ...
Bernard, David J, Chiba-Falek, Ornit, Cronin, Kenneth D, El-Agnaf, Omar MA, Ge, Dongliang, Linnertz, Colton, Manninger, Paul, Nussbaum, Robert L, Orrison, Bonnie M, Rossoshek, Anna, Schlossmacher, Michael G   +10 more
core   +2 more sources

A constitutive active MAPK/ERK pathway due to BRAFV600E positively regulates AHR pathway in PTC [PDF]

, 2015
The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor mediating the toxicity and tumor-promoting properties of dioxin. AHR has been reported to be overexpressed and constitutively active in a variety of solid tumors, but few data
Barollo, Susi, Bertazza, Loris, Bisognin, Andrea, Bortoluzzi, Stefania, Boscaro, Marco, Ceccato, Filippo, Ciato, Denis, Galuppini, Francesca, Guzzardo, Vincenza, Jaffrain Rea, Marie Lise, Mian, Caterina, Occhi, Gianluca, Pennelli, Gianmaria, Regazzo, Daniela, Scaroni, Carla, Vianello, Federica, WATUTANTRIGE FERNANDO, Sara   +16 more
core   +4 more sources