Results 91 to 100 of about 4,794 (208)

Transglycosylation activity of Endo-CC1^N180X.

, 2015
SDS-PAGE analysis of transglycosylation reaction mixtures that contained deglycosylated RNase B, indicated purified Endo-CC1N180X mutant (where X is the altered amino acid residue in the point mutant) and complex type oligosaccharide derived from SGP: 1 ...
Yujiro Higuchi (773330), Yasunari Eshima (773329), Takashi Kinoshita (440727), Shin-ichi Nakakita (595194), Kaoru Takegawa (14399)   +4 more
core   +1 more source

Enzymatic preparation of oligosaccharides by transglycosylation: A comparative study of glucosidases

, 2015
Five glucosidases were studied with respect to their ability to catalyse the transglycosylation of maltose or cellobiose. Experiments were carried out at high substrate concentrations to increase the ratio between transglycosylation and hydrolysis.
Adlercreutz, Patrick, Adlercreutz, Patrick,, Mangas, Juan, Mangas-Sánchez, Juan   +3 more
core   +1 more source

Transglycosylation reactions by variants of Endo-S, Endo-M, and their combinations.

, 2018
(A) Transglycosylation using a mutant alone or a wild-type paired with either a wild-type or a mutant enzyme. (B) Transglycosylation using a pair of mutants. Reactions were carried out using deglycosylated trastuzumab as the acceptor.
Yoshirou Kawaguchi (4885447), Yukiko Sekiguchi (4885450), Mitsuhiro Iwamoto (2680564), Takeshi Honda (150171), Kensuke Nakamura (2989272), Jun Hasegawa (159270)   +5 more
core   +1 more source

A Single Hydrogen Bond Controls the Selectivity of Transglycosylation vs Hydrolysis in Family 13 Glycoside Hydrolases

Converting glycoside hydrolases (GHs) from hydrolytic to synthetic enzymes via transglycosylation is a long-standing goal for the biosynthesis of complex carbohydrates.
Lingqia Su (3150570), Wei Xia (349131), Lei Wang (6656), Sheng Chen (21971), Binju Wang (1419697), Carme Rovira (1275285), Jing Wu (54032), Zhiyong Guo (110695), Isabelle André (1478674)   +8 more
core   +2 more sources

Biochemical characterization of a novel C-terminally truncated β-galactosidase from Paenibacillus antarcticus with high transglycosylation activity

Journal of Dairy Science
: The transgalactosylase activity of β-galactosidases offers a convenient and promising strategy for conversion of lactose into high-value oligosaccharides, such as galactooligosaccharides (GOS) and human milk oligosaccharides.
Jing Li, Jianyu Wang, Qiaojuan Yan, Leying Guan, Shaoqing Yang, Zhengqiang Jiang   +5 more
doaj   +1 more source

Microwave Accelerated Transglycosylation of Rutin by Cyclodextrin Glucanotransferase from Bacillus sp. SK13.002

, 2011
Rutin was subjected to intermolecular transglycosylation assisted with microwave irradiation using cyclodextrin glucanotransferase (CGTase) produced from Bacillus sp. SK13.002. Compared with the conventional enzymatic method for rutin transglycosylation (
Tao Sun, Bo Jiang, Beilei Pan
core   +1 more source

Tuning the Transglycosylation Reaction of a GH11 Xylanase by a Delicate Enhancement of its Thumb Flexibility

, 2021
Glycoside hydrolases (GHs) are attractive tools for multiple biotechnological applications. In conjunction with their hydrolytic function, GHs can perform transglycosylation under specific conditions.
Jeroen Codee, Overkleeft, H.S., Hans van den Elst, Ubbink, M., Marcellus Ubbink, Ben Bdira F., Anneloes Cramer‐Blok, Marneth, K., Fredj Ben Bdira, Cramer-Blok, A.J., Aerts, J.M.F.G., Elst, H. van den, Codee, J.D.C., Kim Marneth, Hermen S. Overkleeft, Johannes M. F. G. Aerts   +15 more
core   +1 more source

Schematic representation of one-pot transglycosylation reactions.

, 2018
(A) Scheme proposed by Li et al. Wild-type Endo-M efficiently hydrolyzes SGP. Hydrolyzed sialoglycan is subsequently converted to SG-oxazoline in situ to provide the active donor substrate for transglycosylation catalyzed by the Endo-S D233Q mutant.
Yoshirou Kawaguchi (4885447), Yukiko Sekiguchi (4885450), Mitsuhiro Iwamoto (2680564), Takeshi Honda (150171), Kensuke Nakamura (2989272), Jun Hasegawa (159270)   +5 more
core   +1 more source

Unexpected Anomeric Acceptor Preference Observed Using dDNP NMR for Transglycosylation Studies of β-Galactosidases

, 2020
The transglycosylation abilities of β-galactosidases were investigated using hyperpolarized [U-13C,U-2H]glucose as an acceptor and o-nitrophenyl β-galactopyranoside as a donor.
Ardenkjær-Larsen, Jan Henrik, Kjeldsen, Christian, Duus, Jens Øllgaard   +2 more
core   +1 more source

Effects of organic solvents on the proportion of hydrolysis, transglycosylation and the product ratio.

, 2014
aValues shown are the means for triplicate experiments. The values of the standard deviations were below 0.5.bTransglycosylation-to-hydrolysis ratio (T:H).Effects of organic solvents on the proportion of hydrolysis, transglycosylation and the product ...
Samson Pachelles (629700), Nor Hasmaliana Abdul Manas (629699), Rosli Md. Illias (601938), Nor Muhammad Mahadi (355153)   +3 more
core   +1 more source