Results 91 to 100 of about 5,064 (210)

Transglycosylation reactions by variants of Endo-S, Endo-M, and their combinations.

, 2018
(A) Transglycosylation using a mutant alone or a wild-type paired with either a wild-type or a mutant enzyme. (B) Transglycosylation using a pair of mutants. Reactions were carried out using deglycosylated trastuzumab as the acceptor.
Yoshirou Kawaguchi (4885447), Yukiko Sekiguchi (4885450), Mitsuhiro Iwamoto (2680564), Takeshi Honda (150171), Kensuke Nakamura (2989272), Jun Hasegawa (159270)   +5 more
core   +1 more source

Biochemical characterization of a novel C-terminally truncated β-galactosidase from Paenibacillus antarcticus with high transglycosylation activity

Journal of Dairy Science
: The transgalactosylase activity of β-galactosidases offers a convenient and promising strategy for conversion of lactose into high-value oligosaccharides, such as galactooligosaccharides (GOS) and human milk oligosaccharides.
Jing Li, Jianyu Wang, Qiaojuan Yan, Leying Guan, Shaoqing Yang, Zhengqiang Jiang   +5 more
doaj   +1 more source

Microwave Accelerated Transglycosylation of Rutin by Cyclodextrin Glucanotransferase from Bacillus sp. SK13.002

, 2011
Rutin was subjected to intermolecular transglycosylation assisted with microwave irradiation using cyclodextrin glucanotransferase (CGTase) produced from Bacillus sp. SK13.002. Compared with the conventional enzymatic method for rutin transglycosylation (
Tao Sun, Bo Jiang, Beilei Pan
core   +1 more source

Deoxynojirimycin enhanced the transglycosylation activity of a glycosidase from the China white jade snail

, 2009
A beta P-D-glycosidase (G1)from the China white Jade snail showed non-Michaelis-Menten mode in catalyzing the reaction using pNPGlu and pNPFuc as the substrate and monitoring the released pNP.
Zhou, Kun, Hu, Ying, Liu, Huixin, Ge, Guangbo, Liu, Yong, Yang, Ling, Luan, Hongwei, Zhang, Yanyan   +7 more
core   +1 more source

A comparative study of hydrolysis and transglycosylation activities of fungal beta-glucosidases

, 2013
β-glucosidases (BGs) from Aspergillus fumigatus, Aspergillus niger, Aspergillus oryzae, Magnaporthe grisea, Neurospora crassa, and Penicillium brasilianum were purified to homogeneity, and investigated for their (simultaneous) hydrolytic and ...
Rune N, Monrad, Præstgaard, Eigil, Westh, Peter, Praestgaard, Jens, Bohlin, Christina Helena, Borch, Kim, Baumann, Martin Johannes   +6 more
core   +1 more source

Effects of organic solvents on the proportion of hydrolysis, transglycosylation and the product ratio.

, 2014
aValues shown are the means for triplicate experiments. The values of the standard deviations were below 0.5.bTransglycosylation-to-hydrolysis ratio (T:H).Effects of organic solvents on the proportion of hydrolysis, transglycosylation and the product ...
Samson Pachelles (629700), Nor Hasmaliana Abdul Manas (629699), Rosli Md. Illias (601938), Nor Muhammad Mahadi (355153)   +3 more
core   +1 more source

Unexpected Anomeric Acceptor Preference Observed Using dDNP NMR for Transglycosylation Studies of β-Galactosidases

, 2020
The transglycosylation abilities of β-galactosidases were investigated using hyperpolarized [U-13C,U-2H]glucose as an acceptor and o-nitrophenyl β-galactopyranoside as a donor.
Ardenkjær-Larsen, Jan Henrik, Kjeldsen, Christian, Duus, Jens Øllgaard   +2 more
core   +1 more source

Schematic representation of one-pot transglycosylation reactions.

, 2018
(A) Scheme proposed by Li et al. Wild-type Endo-M efficiently hydrolyzes SGP. Hydrolyzed sialoglycan is subsequently converted to SG-oxazoline in situ to provide the active donor substrate for transglycosylation catalyzed by the Endo-S D233Q mutant.
Yoshirou Kawaguchi (4885447), Yukiko Sekiguchi (4885450), Mitsuhiro Iwamoto (2680564), Takeshi Honda (150171), Kensuke Nakamura (2989272), Jun Hasegawa (159270)   +5 more
core   +1 more source

Transglycosylation reactions catalyzed by 19 mutants.

, 2015
(a) Lactose self-condensation. Lane 1, standard glucose plus galactose; lane 2, galactose plus lactose; lane 3, lactose. (b) Transglycosylation toward hydroquinone using lactose as donor.
Yuchuan Guo (710628), Guofeng Gu (710631), Lijuan Xu (266140), Lili Lu (408133), Tingting Qi (710630), Dayu Zhang (710629), Min Xiao (320004), Lan Jin (464006), Li Xu (108702)   +8 more
core   +1 more source

THE TRANSGLYCOSYLATION OF KANAMYGIN A

The Journal of Antibiotics, 1972
T, Endo, D, Perlman
openaire   +3 more sources