Results 81 to 90 of about 1,706,590 (302)

Attosecond x-ray transient absorption spectroscopy in graphene

Physical Review Research, 2021
Real-time observations of the coherent electron dynamics induced by ultrashort light pulses in condensed-matter systems have remained elusive due to limitations in existing attosecond devices.
Giovanni Cistaro, Luis Plaja, Fernando Martín, Antonio Picón   +3 more
doaj   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Time-Resolved Spectroscopic Study of N,N-Di(4-bromo)nitrenium Ions in Selected Solutions

Molecules, 2018
Nitrenium ions are important reactive intermediates in chemistry and biology. In this work, femtosecond and nanosecond transient absorption (fs-TA and ns-TA) along with nanosecond time-resolved resonance Raman (ns-TR3) experiments were employed to ...
Lili Du, Xin Lan, Zhiping Yan, Ruixue Zhu, David Lee Phillips   +4 more
doaj   +1 more source

Probing Ionization Dynamics with Attosecond Transient Absorption Spectroscopy [PDF]

, 2016
Attosecond transient absorption experiments are performed to study strong-field ionization dynamics in xenon and methyl bromide. Results in Xe depict half-cycle ionization steps that exhibit an apparent time delay between spin-orbit ionization ...
Stephen R. Leone, Santra, Robin, Mazyar Sabbar, Yi-Jen Chen, Daniel M. Neumark, Timmers, Henry, Chen, Yi-Jen, Leone, Stephen R, Neumark, Daniel M, Henry Timmers, Robin Santra, Sabbar, Mazyar   +11 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Attosecond transient absorption spooktroscopy: a ghost imaging approach to ultrafast absorption spectroscopy

Physical Chemistry Chemical Physics, 2020
Recently demonstrated isolated attosecond XFEL pulses should allow the probing of ultrafast electron dynamics at X-ray wavelengths. The authors use ghost imaging to enable high-resolution transient absorption spectroscopy at fluctuating XFEL sources.
Taran Driver, Siqi Li, Elio G. Champenois, Joseph Duris, Daniel Ratner, Thomas J. Lane, Philipp Rosenberger, Andre Al-Haddad, Vitali Averbukh, Toby Barnard, Nora Berrah, Christoph Bostedt, Philip H. Bucksbaum, Ryan Coffee, Louis F. DiMauro, Li Fang, Douglas Garratt, Averell Gatton, Zhaoheng Guo, Gregor Hartmann, Daniel Haxton, Wolfram Helml, Zhirong Huang, Aaron LaForge, Andrei Kamalov, Matthias F. Kling, Jonas Knurr, Ming-Fu Lin, Alberto A. Lutman, James P. MacArthur, Jon P. Marangos, Megan Nantel, Adi Natan, Razib Obaid, Jordan T. O'Neal, Niranjan H. Shivaram, Aviad Schori, Peter Walter, Anna Li Wang, Thomas J. A. Wolf, Agostino Marinelli, James P. Cryan   +41 more
openaire   +8 more sources

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Characterizing ultrashort pulses with photon energies above 1.12 eV based on transient absorption in silicon thin films

JPhys Photonics
Frequency-resolved optical switching (FROSt) is a phase-matching-free characterization technique for ultrashort pulses based on transient absorption in semiconductors.
Mayank Kumar, Saadat Mokhtari, Tristan Guay, Adrien Leblanc, Kosta Oubrerie, Sohail A Jalil, Elissa Haddad, Gaëtan Jargot, Philippe Lassonde, Heide Ibrahim, Giulio Vampa, François Légaré   +11 more
doaj   +1 more source

Characterization of the key membrane protein cytochrome Cyc2 from Ghiorsea bivora

FEBS Open Bio, EarlyView.
This study reports the first detailed biochemical analysis of Cyc2TAG1_1, a cytochrome protein involved in microbial iron oxidation. We produced and purified this enzyme in E. coli, then measured its iron‐oxidizing efficiency, and characterized its electrical and structural properties.
Josiane Azizi, Alain Dolla, Frauke Baymann, Celine Rommevaux, Latifa Elantak, Helene Gaussier, Matthieu Nouailler   +6 more
wiley   +1 more source

Observation of Hole Transfer through DNA by Monitoring the Transient Absorption of Pyrene Radical Cation

, 2016
Observation of Hole Transfer through DNA by Monitoring the Transient Absorption of Pyrene Radical ...
Sachiko Tojo (1262253), Kiyohiko Kawai (2103679), Tetsuro Majima (1262259), Tadao Takada (1625299), Nobuyuki Ichinose (2214910)   +4 more
core   +2 more sources