Results 191 to 200 of about 1,253,450 (306)

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Efficient production of gene-edited onion (Allium cepa) plants using biolistic delivery of cas9 RNPs and transient expression constructs. [PDF]

Plant Cell Rep
De La Mora CL, Havey MJ, Krysan PJ.
europepmc   +1 more source

Transient expression of antinuclear RNP-A antibodies in patients with acute COVID-19 infection. [PDF]

J Transl Autoimmun, 2022
Zhou S, Kaul R, Lynch KL, Wu AHB, Walker RP.   +4 more
europepmc   +1 more source

The skills required for transition to university and study in biological sciences: A student perspective

FEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

Harnessing Transient Expression Systems with Plant Viral Vectors for the Production of Biopharmaceuticals in <i>Nicotiana benthamiana</i>. [PDF]

Int J Mol Sci
Akher SA, Wang KY, Hall K, Hunpatin OS, Shan M, Zhang Z, Guo Y.   +6 more
europepmc   +1 more source

Isolation of 5' regulatory region of COLD1 gene and its functional characterization through transient expression analysis in tobacco and sugarcane. [PDF]

3 Biotech, 2023
Mohanan MV, Pushpanathan A, Jayanarayanan AN, Selvarajan D, Ramalingam S, Govind H, Chinnaswamy A.   +6 more
europepmc   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Transient Expression of Zika NS2B Protein Antigen in Nicotiana benthamiana and Use for Arboviruses Diagnosis. [PDF]

ACS Omega
Herazo MAM, Dantas DRS, Silva BB, Costa HPS, Santos ENFN, Moura LFWG, Neto JXS, Van Tilburg MF, Florean EOPT, Moura AA, Guedes MIF.   +10 more
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Transient Expression of Hen Egg White Lysozyme (EWL) in <i>Nicotiana benthamiana</i> Influences Plant Pathogen Infection. [PDF]

Life (Basel)
Meng Z, Wang H, Jia C, Chen G, Zhao M.
europepmc   +1 more source