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Transient Gene Expression in Medicago truncatula Leaves via Agroinfiltration
Methods in Molecular Biology, 2013 Transient expression is a powerful method for the functional characterization of genes. In this chapter, we outline a protocol for the transient expression of constructs in Medicago truncatula leaves using Agrobacterium tumefaciens infiltration.
Kelsey L. Picard +2 more
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Transient High Altitude Expressive Aphasia
High Altitude Medicine & Biology, 2000Transient focal neurological deficits have been described in sojourners to high altitude. We present two cases of transient expressive aphasia in well-acclimatized high altitude climbers. We speculate that this type of transient focal neurological impairment may represent migraine aura, and we discuss other reports of transient focal neurological ...
T E, Dietz, V H, McKiel
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Scalable Transient Protein Expression
2013Transient transfection is a well-established method to rapidly express recombinant proteins from mammalian cells. Accelerating activity in biotherapeutic drug development, demand for protein-based reagents, vaccine research, and large initiatives in structural and functional studies of proteins have propelled the need to generate moderate to high ...
Henry C, Chiou +5 more
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Transient Expression in Protoplasts
Cold Spring Harbor Protocols, 2007INTRODUCTIONThe study of transient gene expression provides a useful complement to the study of stably transformed plants. Transient assays offer a quick method of testing the effects of genes, using either phenotypic, molecular, or biochemical readouts.
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Transient gene expression in untransformed lymphocytes
European Journal of Immunology, 1987AbstractConditions for transfection of normal, freshly isolated and mitogen‐stimulated spleen cells were established. A sharp optimum of plasmid at 10 μg per 1.5 × 107 transfected spleen cells per transfection was observed. The Ig promoter and the IgH enhancer activity was found to be restricted to cells of the B lineage also in normal, freshly ...
I L, Mårtensson, T, Leanderson
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Transient expression in tobacco leaves
Trends in Plant Science, 2000Chimeric heavy and light chain genes of tumor-specific antibodies have been constructed and cloned into two independent plant expression vectors. In vivo assembly of full-size antibodies has been achieved by the simultaneous expression of light and heavy chains after the vacuum infiltration of tobacco leaves with two populations of recombinant ...
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Transient Gene Expression Levels from Multigene Expression Vectors
Biotechnology Progress, 2007AbstractMultigene expression vectors are commonly utilized whereby the (over)expression of two or more genes is desired simultaneously and often at supposedly equivalent levels. We have characterized dual‐gene and pEE14.4 RlucMFluc expression plasmids in which the second hCMV‐MIE promoter is replaced with a c‐myc IRES to enable one‐step coordinated ...
Underhill, MF +4 more
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Transient Expression Assays in Tobacco Protoplasts
2013The sequence information generated through genome and transcriptome analysis from plant tissues has reached unprecedented sizes. Sequence homology-based annotations may provide hints for the possible function and roles of particular plant genes, but the functional annotation remains nonexistent or incomplete for many of them. To discover gene functions,
Robin, Vanden Bossche +3 more
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Transient Protein Expression by Agroinfiltration in Lettuce
2016Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant ...
Qiang, Chen +6 more
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Large scale transient expression with COS cells
Cytotechnology, 1996We demonstrate here that transient expression with COS cells can be performed at the one litre scale for a period of more than 10 days. Cells grown in T225 flasks were transfected by electroporation, transferred into spinners, and then grown either in suspension or on microcarriers.
H D, Blasey +3 more
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