Results 251 to 260 of about 1,253,450 (306)

[18F]Fluorodeprenyl‐D2 PET as a Tool to Monitor Disease Activity in GAD65‐Ab Autoimmune Encephalitis

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To evaluate [18F]fluorodeprenyl‐D2 ([18F]F‐DED) positron‐emission tomography (PET) imaging as a biomarker of disease activity in autoimmune encephalitis (AIE) associated with glutamic acid decarboxylase 65 (GAD65) antibodies. Methods [18F]F‐DED PET was performed in 25 GAD65‐AIE patients and 8 controls using dynamic (0–60 min) and ...
Julia S. Dorneich, Jonathan A. Gernert, Hanna Zimmermann, Lisa Tagnin, Marianthi Zeinaki, Laura Sanzo, Letizia Vogler, Elisabeth Kaufmann, Justina Dargvainiene, Frank Leypoldt, Gérard N. Bischof, Robert Perneczky, Boris‐Stephan Rauchmann, Simon Lindner, Günter U. Höglinger, Rudolf A. Werner, Martin Kerschensteiner, Tania Kümpfel, Matthias Brendel, Franziska S. Thaler   +19 more
wiley   +1 more source

GAD65 Antibody ELISA With Extended Reportable Range: Validation and Guidance for Neurological Practice

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To (1) validate GAD65‐ELISA detection and quantification for type 1 diabetes mellitus and autoimmune neurological diagnoses, (2) correlate ELISA results (reference range < 5 IU/mL) with established radioimmunoprecipitation assay (RIA; ≤ 0.02 nmol/L), and (3) define ELISA clinical utility and pitfalls.
Andrew McKeon, Dana Olofson, Diana Anissian, Divyanshu Dubey, Eoin P. Flanagan, Max C. Herman, Sarosh R. Irani, Daniel H. Lachance, Maria A. Willrich, Sean Pittock, John R. Mills, Anastasia Zekeridou   +11 more
wiley   +1 more source

High‐Resolution MRI Revealed Different Etiology‐Specific Associations With Cerebral Infarction in Adult Moyamoya Vasculopathy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective High‐resolution MRI enables detailed assessment of intracranial vessel wall pathology in moyamoya vasculopathy. We aimed to classify adult moyamoya vasculopathy etiologies using high‐resolution MRI and to examine subtype‐specific associations between high‐resolution MRI features and ischemic infarction.
Guangsong Han, Jiachun Pan, Yuehui Hong, Xiaoyuan Fan, Ming Yao, Lixin Zhou, Yicheng Zhu, Feng Feng, Jun Ni   +8 more
wiley   +1 more source

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Transient expression of recombinant proteins in plants

2021
Transient protein expression in plant cells is less time consuming than the production of whole transgenic plants. For transient expression, agroinfiltration is a simple and effective method to deliver transgenes into plant cells. After an Agrobacterium infection, recombinant proteins can be produced in plant cells from 3 to 10days. To increase protein
Shohei, Nosaki, Kenji, Miura
openaire   +2 more sources

Transient High Altitude Expressive Aphasia

High Altitude Medicine & Biology, 2000
Transient focal neurological deficits have been described in sojourners to high altitude. We present two cases of transient expressive aphasia in well-acclimatized high altitude climbers. We speculate that this type of transient focal neurological impairment may represent migraine aura, and we discuss other reports of transient focal neurological ...
T E, Dietz, V H, McKiel
openaire   +2 more sources

Scalable Transient Protein Expression

2013
Transient transfection is a well-established method to rapidly express recombinant proteins from mammalian cells. Accelerating activity in biotherapeutic drug development, demand for protein-based reagents, vaccine research, and large initiatives in structural and functional studies of proteins have propelled the need to generate moderate to high ...
Henry C, Chiou, Sanjay, Vasu, Chao Yan, Liu, Isabel, Cisneros, Meredith B, Jones, Jonathan F, Zmuda   +5 more
openaire   +2 more sources

Transient gene expression in untransformed lymphocytes

European Journal of Immunology, 1987
AbstractConditions for transfection of normal, freshly isolated and mitogen‐stimulated spleen cells were established. A sharp optimum of plasmid at 10 μg per 1.5 × 107 transfected spleen cells per transfection was observed. The Ig promoter and the IgH enhancer activity was found to be restricted to cells of the B lineage also in normal, freshly ...
I L, Mårtensson, T, Leanderson
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Transient Expression in Protoplasts

Cold Spring Harbor Protocols, 2007
INTRODUCTIONThe study of transient gene expression provides a useful complement to the study of stably transformed plants. Transient assays offer a quick method of testing the effects of genes, using either phenotypic, molecular, or biochemical readouts.
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Transient Expression Assay by Agroinfiltration of Leaves

2006
An Agrobacterium-mediated transient expression assay has been described for in vivo analysis of constitutive or inducible gene expression in Arabidopsis plants. By simple infiltration of Agrobacterium cells carrying appropriate gene constructs into Arabidopsis leaves, transient expression assays can be performed within 3 d without using expensive ...
Min Woo, Lee, Yinong, Yang
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Transient Gene Expression Levels from Multigene Expression Vectors

Biotechnology Progress, 2007
AbstractMultigene expression vectors are commonly utilized whereby the (over)expression of two or more genes is desired simultaneously and often at supposedly equivalent levels. We have characterized dual‐gene and pEE14.4 RlucMFluc expression plasmids in which the second hCMV‐MIE promoter is replaced with a c‐myc IRES to enable one‐step coordinated ...
Underhill, MF, Smales, CM, Naylor, LH, Birch, JR, James, DC   +4 more
openaire   +4 more sources