Results 261 to 270 of about 1,253,450 (306)

Transient expression in tobacco leaves

Trends in Plant Science, 2000
Chimeric heavy and light chain genes of tumor-specific antibodies have been constructed and cloned into two independent plant expression vectors. In vivo assembly of full-size antibodies has been achieved by the simultaneous expression of light and heavy chains after the vacuum infiltration of tobacco leaves with two populations of recombinant ...
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Transient Expression Assays in Tobacco Protoplasts

2013
The sequence information generated through genome and transcriptome analysis from plant tissues has reached unprecedented sizes. Sequence homology-based annotations may provide hints for the possible function and roles of particular plant genes, but the functional annotation remains nonexistent or incomplete for many of them. To discover gene functions,
Robin, Vanden Bossche, Brecht, Demedts, Rudy, Vanderhaeghen, Alain, Goossens   +3 more
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Transient Protein Expression by Agroinfiltration in Lettuce

2016
Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant ...
Qiang, Chen, Matthew, Dent, Jonathan, Hurtado, Jake, Stahnke, Alyssa, McNulty, Kahlin, Leuzinger, Huafang, Lai   +6 more
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TauG-guidance of transients in expressive musical performance

Experimental Brain Research, 2008
The sounds in expressive musical performance, and the movements that produce them, offer insight into temporal patterns in the brain that generate expression. To gain understanding of these brain patterns, we analyzed two types of transient sounds, and the movements that produced them, during a vocal duet and a bass solo.
Schogler, Benjaman, Pepping, Gert-Jan, Lee, David N.   +2 more
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Development of a spermine lipid for transient antibody expression

Bioorganic & Medicinal Chemistry, 2023
Transient expression is the only way to quickly obtain a small scale of antibodies for biomedical research and therapeutic evaluation. The agents for transfecting the suspension cells, e.g. PEI or commercial agents, either lack efficiency or excessively expensive.
Shaowei Sui, Hao Wang, Jiajie Song, Wanyi Tai   +3 more
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Large scale transient expression with COS cells

Cytotechnology, 1996
We demonstrate here that transient expression with COS cells can be performed at the one litre scale for a period of more than 10 days. Cells grown in T225 flasks were transfected by electroporation, transferred into spinners, and then grown either in suspension or on microcarriers.
H D, Blasey, J P, Aubry, G J, Mazzei, A R, Bernard   +3 more
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Transient gene expression in electroporated Solanum protoplasts

Plant Molecular Biology, 1989
Electroporation was used to evaluate parameters important in transient gene expression in potato protoplasts. The protoplasts were from leaves of wild potato Solanum brevidens, and from leaves, tubers and suspension cells of cultivated Solanum tuberosum cv. Désirée. Reporter enzyme activity, chloramphenicol acetyl transferase (CAT) under the control of
Jones, H., Ooms, G., Jones, M.G.K.
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Transient expression of doublecortin during adult neurogenesis

Journal of Comparative Neurology, 2003
AbstractDuring development of the central nervous system, expression of the microtubule binding protein doublecortin (DCX) is associated with migration of neuroblasts. In addition to this developmental role, expression of DCX remains high within certain areas of the adult mammalian brain.
Jason P, Brown, Sébastien, Couillard-Després, Christiana M, Cooper-Kuhn, Jürgen, Winkler, Ludwig, Aigner, H Georg, Kuhn   +5 more
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In Planta Transient Expression Analysis of Monoterpene Synthases

2012
In vitro-based analyses of monoterpene synthase (mono-TPS) enzymes have led to a wealth of knowledge regarding their catalytic behavior, the mechanistic principles governing their product specificity, and the molecular basis for their evolution.
Sol A, Green, Xiuyin, Chen, Adam J, Matich   +2 more
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Variations in expression and treatment of transient neurocardiogenic instability

The American Journal of Cardiology, 1992
Whereas previous reports describe the head-up tilt test for the diagnosis of neurocardiogenic syncope, this study focuses on the events leading up to syncope. These events are part of a spectrum of neurocardiogenic instability; syncope is the extreme end point.
O G, Thilenius, K J, Ryd, J, Husayni
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