Identifying parameters to improve the reproducibility of transient gene expression in High Five cells. [PDF]
PLoS One, 2019 Bleckmann M +7 more
europepmc +1 more source
Advanced Functional Materials, EarlyView.A chiral photodetector capable of selectively distinguishing left‐ and right‐handed circularly polarized light is experimentally demonstrated. The device, which features a nanopatterned electrode inverse‐designed by a genetic algorithm within a metal–dielectric–metal nanocavity that incorporates a vacuum‐deposited small‐molecule multilayer, exhibits ...
Kyung Ryoul Park +3 more
wiley +1 more source
An Efficient and Economical Protocol for Isolating, Purifying and PEG-Mediated Transient Gene Expression of Chinese Kale Hypocotyl Protoplasts. [PDF]
Plants (Basel), 2019 Sun B +12 more
europepmc +1 more source
Cardiac‐Derived ECM Microspheres for Enhanced hiPSC‐CMs Maturation
Advanced Functional Materials, EarlyView.Cardiac extracellular matrix microspheres derived from decellularized porcine heart provide a biomimetic 3D microenvironment for human induced pluripotent stem cell–derived cardiomyocytes (hiPSC‐CMs). This platform supports short‐ and long‐term culture, enhances structural organization, and promotes electrophysiological and functional maturation of ...
Jiazhu Xu +9 more
wiley +1 more source
Regeneration and transient gene expression in protoplasts of Draparnaldia (chlorophytes), an emerging model for comparative analyses with basal streptophytes. [PDF]
Plant Methods, 2019 Caisová L, Caisová L, Jobe TO.
europepmc +1 more source
Advanced Functional Materials, EarlyView.This study introduces VIVID (Vesicle In Vivo Identification using DNA), a qPCR‐based platform that tracks PCR‐amplifiable DNA tags loaded in the EVs for accurate and quantifiable EV biodistribution in vivo. ABSTRACT Extracellular vesicles (EVs) represent promising carriers for nucleic acid therapeutics, offering advantages over synthetic nanoparticles ...
Oscar Boyadjian +5 more
wiley +1 more source
Agrobacterium-Mediated Transient Gene Expression in Developing Ricinus communis Seeds: A First Step in Making the Castor Oil Plant a Chemical Biofactory. [PDF]
Front Plant Sci, 2019 Sánchez-Álvarez A +5 more
europepmc +1 more source
Advanced Functional Materials, EarlyView.We have developed DEX/GM, an all‐natural, personalizable hybrid vaccine designed by coating dendritic cell‐derived exosomes (DEX) onto tumor cell membranes (GM) for sustained prophylaxis against glioblastoma (GBM). ABSTRACT Glioblastoma (GBM), one of the most aggressive and lethal brain tumors, remains incurable with a poor clinical prognosis.
Shanshan Li +6 more
wiley +1 more source
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses. [PDF]
J Vis Exp, 2018 Wu F, Hanzawa Y.
europepmc +1 more source
Advanced Functional Materials, EarlyView.Micro‐injection laser‐assisted bioprinting enables ultrafast and precise patterning of small endothelial cell spheroids by injecting a highly concentrated single‐cell suspension into GelMA/ColMA hydrogels. In co‐culture with fibroblasts, controlled pre‐vasculogenic network formation is obtained at microscale resolution.
Charles Handschin +9 more
wiley +1 more source