Results 151 to 160 of about 3,061,365 (279)
The impact of leukocyte‐associated RNA retention on platelet RNA‐based cancer detection
Molecular Oncology, EarlyView.Platelets carry RNA signals that may help detect cancer, but platelet samples can also retain RNA from white blood cells. We found that this retained white blood cell signal did not significantly affect machine learning cancer classification, suggesting that the cancer‐related RNA signal in platelets is largely independent of leukocyte‐associated RNA ...
Michał Sieczczyński +12 more
wiley +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
UMCCTS Newsletter, September 2024
This is the September 2024 issue of the UMass Center for Clinical and Translational Science Newsletter containing news and events of interest.Supported by the National Center for Advancing Translational Sciences, National Institutes of Health, through ...
UMass Center for Clinical and Translational Science
core +1 more source
FEBS Open Bio, EarlyView.UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Small RNA pathways in mammalian oocytes
FEBS Open Bio, EarlyView.Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley +1 more source
Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA
FEBS Open Bio, EarlyView.PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro +1 more
wiley +1 more source
Translational Medicine Symposium 2017- Speaker Biographies
, 2017 Biographies for the speakers at the Translational Medicine Symposium ...
L. Bhoite, PhD; K. Kuck, PhD; T. Petelenz, PhD; G. Prestwich, PhD; C. Coppin, MD, PhD; S. Fojtik, MA; J. Hotaling, MD, MS; J. Langell, MD, PhD; J. Langell, MD, PhD; D. Sakata, MD; P. Taylor; P. Triolo, PhD, RAC; R. Looper, PhD; S. Marty, PhD; J. Rutter, PhD; J. Schiffman; S. Sharma, MA, MBA, FACP; J. Skidmore; G. Zentner, PhD; E. Braendle, MD, PhD, CEO/President, ARUP Labs; C. Hensel, PhD; L. Jenkins; N. Kusukawa, PhD; M. Langer, JD; S. Owen, PhD; A. Ward, PhD; T. Andersen, PhD, MSW, MS, LCSW; B. Chortkoff, MD; B. Elieson, MBA; J. Facelli, PhD; J. Hurdle, MD, PhD; C. Wasden, EdD, MBA; K. Wethington, MD; M. Lehmkuhle, PhD; E. Paulsen, PhD; F. Solzbacher, PhD; C. Testa, PhD; C. Testa, PhD; J. Thompson, Director Engineering Team, Technology and Venture Commercialization, University of Utah; A. Welm, PhD; M. Yu, PhD
core
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source