Results 101 to 110 of about 68,477 (364)

Paternal Circadian Disruption Impairs Offspring Cognition via Sperm microRNAs

Advanced Science, EarlyView.
Paternal circadian disruption remodels the sperm small RNA payload, elevating miR‐92a‐3p/miR‐25‐3p levels and perturbing early embryonic gene regulatory programs. Microinjection experiments and single‐embryo transcriptomics reveal sex‐specific developmental vulnerabilities, ultimately impairing offspring hippocampal synaptic plasticity and cognition ...
Kexin Zou, Sisi Luo, Binliang Tang, Zhiqiang Liu, Yuchuan Zhou, Yicong Meng, Zhengmu Wu, Zheng Sun, Weihui Shi, Jianzhong Sheng, Chuanjin Yu, Zhenhua Li, Siyi Wei, Mo Zhang, Yujie Luo, Jing Yan, Xueyun Qin, Jiahang Mo, Chengliang Zhou, Jinsong Li, Hefeng Huang, Yifu Ding, Guolian Ding   +22 more
wiley   +1 more source

CAR T cell engineering impacts antigen-independent activation and co-inhibition

Molecular Therapy: Methods & Clinical Development
Viral vectors have successfully modified T cells to express chimeric antigen receptors (CARs), leading to clinical approvals. However, their high cost and regulatory challenges hinder rapid clinical translation. Here, we demonstrate that our lentivirally
Christoph Schultheiß, Simon Stücheli, Brenda Besemer, Maja Kadel, Paul Schmidt-Barbo, Andreas Zingg, Natascha Franz, Sarah Adamo, Frank Stenner, Claudia Fischer, Heinz Läubli, Mascha Binder   +11 more
doaj   +1 more source

Temporal self-regulation of transposition through host-independent transposase rodlet formation

Nucleic Acids Research, 2016
Transposons are highly abundant in eukaryotic genomes, but their mobilization must be finely tuned to maintain host organism fitness and allow for transposon propagation.
Lauren E. Woodard, Laura M. Downes, Yi-Chien Lee, A. Kaja, Eyuel Terefe, Matthew H. Wilson   +5 more
semanticscholar   +1 more source

Functional characterization of an active Rag-like transposase [PDF]

Nature Structural & Molecular Biology, 2012
The formation of diverse immunoglobulin genes results in part from Rag protein-mediated DNA double-strand breaks at the edges of immunoglobulin gene segments, followed by combinatorial reassembly of these segments. We report that a Transib transposase from the insect Helicoverpa zea is active in vitro and that its breakage and joining activities mimic ...
Hencken, Cary G., Li, Xianghong, Craig, Nancy L.   +2 more
openaire   +2 more sources

Understanding and Overcoming Antibody‐Drug Conjugate Resistance: Biological Mechanisms and Emerging Analytical Frameworks in Breast Cancer

Advanced Science, EarlyView.
Antibody–drug conjugates (ADCs) transform breast cancer therapy, yet resistance limits their durability. Emerging evidence reveals that ADC failure is not solely tumor‐intrinsic but shaped by dynamic tumor–microenvironment interactions that alter drug delivery, processing, and response.
Minji Seo, Jangsoon Lee, Naoto T. Ueno
wiley   +1 more source

Hybrid adeno-associated viral vectors utilizing transposase-mediated somatic integration for stable transgene expression in human cells.

PLoS ONE, 2013
Recombinant adeno-associated viral (AAV) vectors have been shown to be one of the most promising vectors for therapeutic gene delivery because they can induce efficient and long-term transduction in non-dividing cells with negligible side-effects ...
Wenli Zhang, Manish Solanki, Nadine Müther, Melanie Ebel, Jichang Wang, Chuanbo Sun, Zsuzsanna Izsvak, Anja Ehrhardt   +7 more
doaj   +1 more source

Self‐Assembling Hybrid Hydrogel Reprograms the Stromal Vascular Fraction to Treat Osteoarthritis

Advanced Science, EarlyView.
This study presents a bioinspired injectable hydrogel that enhances the therapeutic potential of stem cell‐rich stromal vascular fraction for treating osteoarthritis. By reprogramming cell behavior through epigenetic modulation, the hydrogel promotes cartilage regeneration and reduces joint damage in a rat model, offering a promising new approach for ...
Waifang Hou, Yongfeng Chen, Longyou Xiao, Wei Qi, Tianshu Du, Qiang Sun, Borui Xue, Qinghe Zhao, Pengfei Xie, Zi Ye, Fei Han, Lingli Guo, Yang Jiao, Ranran Zhang, Hong Jia, Xiao Huang, Jianwei Guo, Peng Wang, Huayi Wang, Yuanrui Wang, Wenjing Chen, Haining Wu, Liumin He, Dawei Zhang   +23 more
wiley   +1 more source

Structure, Activity, and Function of SETMAR Protein Lysine Methyltransferase

Life, 2021
SETMAR is a protein lysine methyltransferase that is involved in several DNA processes, including DNA repair via the non-homologous end joining (NHEJ) pathway, regulation of gene expression, illegitimate DNA integration, and DNA decatenation.
Michael Tellier
doaj   +1 more source

Tn 5 Transposase Active Site Mutants [PDF]

Journal of Biological Chemistry, 2002
Tn5 transposase (Tnp) is a 53.3-kDa protein that is encoded by and facilitates movement of transposon Tn5. Tnp monomers contain a single active site that is responsible for catalyzing a series of four DNA breaking/joining reactions at one transposon end.
Todd A, Naumann, William S, Reznikoff
openaire   +2 more sources

Organ‐Specific and Conserved Regulatory Logic Orchestrates Gene Expression in the Embryonic Mesothelium

Advanced Science, EarlyView.
Integrated multi‐omic profiling maps the gene‐regulatory landscape of the coelomic mesothelium across heart, lung, and pancreas. A cardiac‐restricted regulatory program is uncovered in which TBX20 activates heart mesothelial (epicardial) cis‐regulatory elements, while MAF emerges as a conserved regulator of mesothelial identity.
Quang Minh Dang, Nicola Smart, Andia Nicole Redpath, Joaquim Miguel Vieira   +3 more
wiley   +1 more source