Results 91 to 100 of about 68,477 (364)

Subtype‐specific enhancer RNAs define transcriptional regulators and prognosis in breast cancers

Molecular Oncology, EarlyView.
This study employed machine learning methodologies to perform the subtype‐specific classification of RNA‐seq data sets, which are mapped on enhancers from TCGA‐derived breast cancer patients. Their integration with gene expression (referred to as ProxCReAM eRNAs) and chromatin accessibility profiles has the potential to identify lineage‐specific and ...
Aamena Y. Patel, Peyman Zarrineh, Nathnael T. Tuffa, Jigar H. Sheth, Sumitra Mohan, Mudassar Iqbal, Sankari Nagarajan   +6 more
wiley   +1 more source

A transposase strategy for creating libraries of circularly permuted proteins

Nucleic Acids Research, 2012
A simple approach for creating libraries of circularly permuted proteins is described that is called PERMutation Using Transposase Engineering (PERMUTE).
M. Mehta, Shirley Liu, J. Silberg
semanticscholar   +1 more source

A transposase-independent mechanism gives rise to precise excision of IS256 from insertion sites in Staphylococcus epidermidis.

, 2007
The mobile element IS256 causes phase variation of biofilm formation in Staphylococcus epidermidis by insertion and precise excision from the icaADBC operon.
Ziebuhr, Wilma, Wilma Ziebuhr, Susanne Hennig, A Transposase-independent Mechanism   +3 more
core   +1 more source

Hyperactive self-inactivating piggyBac for transposase-enhanced pronuclear microinjection transgenesis [PDF]

, 2012
We have developed a unique method for mouse transgenesis. The transposase-enhanced pronuclear microinjection (PNI) technique described herein uses the hyperactive piggyBac transposase to insert a large transgene into the mouse genome.
Pelczar, Pawel, Marh, Joel, Stoytchev, Ilko, Yamashiro, Hideaki, Urschitz, Johann, Moisyadi, Stefan, Sugawara, Atsushi, Stoytcheva, Zoia, Owens, Jesse B, Yanagimachi, Ryuzo   +9 more
core   +1 more source

Presence of large number of transposase and transposase helper genes in a bacterial species meant that it is not suited as biotechnology chassis

, 2023
Transposase are “jumping” genes that excise themselves from one location in the genome to move to another. Thus, insertion of transposase genes into a new site in the genome may cause disruptions to gene function and mutability.
Wenfa Ng (9593279)
core   +1 more source

Epigenetic heterogeneity and plasticity in therapy‐induced tumor states through single‐cell multi‐omics

Molecular Oncology, EarlyView.
Single‐cell multi‐omics reveals epigenetic heterogeneity across therapy‐adaptive tumor states, including quiescent/dormant, drug‐tolerant persister, and EMT‐like phenotypes. By linking regulatory features with state‐associated biomarkers, these approaches inform biomarker‐guided therapeutic strategies for evolving tumors.
Hee Jung Kim, Hwiyeong Lee, Jin Hong, Daechan Park   +3 more
wiley   +1 more source

Insertion sequence content reflects genome plasticity in strains of the root nodule actinobacterium Frankia

BMC Genomics, 2009
Background Genome analysis of three Frankia sp. strains has revealed a high number of transposable elements in two of the strains. Twelve out of the 20 major families of bacterial Insertion Sequence (IS) elements are represented in the 148 annotated ...
Tisa Louis S, Normand Philippe, Lapierre Pascal, Gogarten Johann P, Bickhart Derek M, Benson David R   +5 more
doaj   +1 more source

In vivo mutagenesis of bacteriophage Mu transposase [PDF]

Journal of Bacteriology, 1987
We devised a method for isolating mutations in the bacteriophage Mu A gene which encodes the phage transposase. Nine new conditional defective A mutations were isolated. These, as well as eight previously isolated mutations, were mapped with a set of defined deletions which divided the gene into 13 100- to 200-base-pair segments.
Toussaint, Ariane, Desmet, Lucie, Faelen, Michel, Alazard, Robert, Chandler, Mick, Pato, Martin Leon   +5 more
openaire   +3 more sources

Anti‐PD‐1 Nanobody‐Armored MSLN CAR‐T Therapy for Malignant Mesothelioma: Preclinical and Clinical Studies

Advanced Science, EarlyView.
A novel therapy using engineered immune cells (NAC‐T cells) showed promise for refractory malignant mesothelioma. Based on the encouraging preclinical data, the first‐in‐human trial is initiated, demonstrating tolerable safety and promising anti‐tumor activity (ORR 63.6%, DCR 100%, including one CR).
Yan Sun, Haochen Yang, Qing Xu, Xingya Li, Jinxing Lou, Jianchun Duan, Jiachen Xu, Zhuqing Liu, Yong Xia, Zhicai Lin, Linlin Li, Dan Sun, Jiaguo Li, Tao Liu, Jun Guo, Wenfeng Xu, Weimin Zhu, Yi Liu, Boyang Sun, Jia Zhong, Lijie Rong, Qijun Qian, Chenqi Xu, Jie Wang   +23 more
wiley   +1 more source

Random Insertion Reporter Gimmicks Powered by Cut-and-Paste DNA Transposons

Biomedicines
Transposons are mobile genetic elements capable of moving within the genome. Leveraging this property—particularly the cut-and-paste mechanism of DNA transposons—has enabled the development of technologies for inserting exogenous DNA fragments into host ...
Yamato Kasahara, Kentaro Semba, Shinya Watanabe, Kosuke Ishikawa   +3 more
doaj   +1 more source