Results 71 to 80 of about 68,477 (364)
Table3_Insertion Specificity of the hATx-6 Transposase of Hydra magnipapillata.DOCX
, 2021 Transposable elements (TE) are mobile genetic elements, present in all domains of life. They commonly encode a single transposase enzyme, that performs the excision and reintegration reactions, and these enzymes have been used in mutagenesis and creation
Laurence Ettwiller (15718) +5 more
core +1 more source
bioRxiv, 2019 Next-generation sequencing of single-stranded DNA (ssDNA) enables transgene characterization of gene-therapy vectors such as adeno-associated virus (AAV), but current library generation uses complicated and potentially biased second-strand synthesis.
M. Radukic +4 more
semanticscholar +1 more source
Simplified Epigenome Profiling Using Antibody-tethered Tagmentation
Bio-Protocol, 2021 We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples and ...
Steven Henikoff +2 more
doaj +1 more source
Image1_Insertion Specificity of the hATx-6 Transposase of Hydra magnipapillata.TIF
, 2021 Transposable elements (TE) are mobile genetic elements, present in all domains of life. They commonly encode a single transposase enzyme, that performs the excision and reintegration reactions, and these enzymes have been used in mutagenesis and creation
Laurence Ettwiller (15718) +5 more
core +1 more source
bioRxiv, 2018 Simultaneous measurement of cell lineage and cell fates is a longstanding goal in biomedicine. Here we describe EMBLEM, a strategy to track cell lineage using endogenous mitochondrial DNA variants in ATAC-seq data.
Jin Xu +6 more
semanticscholar +1 more source
Comparative sequence analysis of IS50/Tn5 transposase [PDF]
, 2004 Author Posting. © American Society for Microbiology, 2004. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution.
Apodaca, Jennifer +5 more
core +1 more source
Structures of a RAG-like transposase during cut-and-paste transposition
Nature, 2019 Transposons have had a pivotal role in genome evolution1 and are believed to be the evolutionary progenitors of the RAG1–RAG2 recombinase2, an essential component of the adaptive immune system in jawed vertebrates3.
Chang Liu, Yang Yang, D. Schatz
semanticscholar +1 more source
Wellcome Open Research, 2021 Background Genetic systems have been developed for Chlamydia but the extremely low transformation frequency remains a significant bottleneck. Our goal is to develop a self-replicating transposon delivery vector for C.
Rachel J. Skilton +4 more
doaj +1 more source
Mechanism and regulation of P element transposition [PDF]
Open Biology, 2020 P elements were first discovered in the fruit fly Drosophila melanogaster as the causative agents of a syndrome of aberrant genetic traits called hybrid dysgenesis.
George E. Ghanim +2 more
doaj +1 more source
Structure of a P element transposase–DNA complex reveals unusual DNA structures and GTP-DNA contacts
Nature Structural & Molecular Biology, 2019 P element transposase catalyzes the mobility of P element DNA transposons within the Drosophila genome. P element transposase exhibits several unique properties, including the requirement for a guanosine triphosphate cofactor and the generation of long ...
George E. Ghanim +3 more
semanticscholar +1 more source