Results 61 to 70 of about 77,105 (268)

Random Insertion Reporter Gimmicks Powered by Cut-and-Paste DNA Transposons

Biomedicines
Transposons are mobile genetic elements capable of moving within the genome. Leveraging this property—particularly the cut-and-paste mechanism of DNA transposons—has enabled the development of technologies for inserting exogenous DNA fragments into host ...
Yamato Kasahara, Kentaro Semba, Shinya Watanabe, Kosuke Ishikawa   +3 more
doaj   +1 more source

Insertion sequence content reflects genome plasticity in strains of the root nodule actinobacterium Frankia

BMC Genomics, 2009
Background Genome analysis of three Frankia sp. strains has revealed a high number of transposable elements in two of the strains. Twelve out of the 20 major families of bacterial Insertion Sequence (IS) elements are represented in the 148 annotated ...
Tisa Louis S, Normand Philippe, Lapierre Pascal, Gogarten Johann P, Bickhart Derek M, Benson David R   +5 more
doaj   +1 more source

Comparative genomic analyses reveal a lack of a substantial signature of host adaptation in Rhodococcus equi (‘Prescottella equi’) [PDF]

, 2013
Rhodococcus equi (‘Prescottella equi’) is a pathogenic actinomycete primarily infecting horses but has emerged as an opportunistic human pathogen. We have sequenced the genome of the type strain of this species, R.
Arruda, Aziz, Aziz, Clark, Darling, Giguere, Goodfellow, Grant, Jones, Jones, Jones, Kedlaya, Kämpfer, Lai, Law, Letek, Letek, Lowder, McLeod, Mohn, Pena-Moctezuma, Prescott, Prescott, Resch, Reumerman, Sangal, Sangal, Segata, Sekine, Takai, Takai, Teufel, Vazquez-Boland, Vera-Cabrera, Vernikos, Yamshchikov   +35 more
core   +1 more source

Anti‐PD‐1 Nanobody‐Armored MSLN CAR‐T Therapy for Malignant Mesothelioma: Preclinical and Clinical Studies

Advanced Science, EarlyView.
A novel therapy using engineered immune cells (NAC‐T cells) showed promise for refractory malignant mesothelioma. Based on the encouraging preclinical data, the first‐in‐human trial is initiated, demonstrating tolerable safety and promising anti‐tumor activity (ORR 63.6%, DCR 100%, including one CR).
Yan Sun, Haochen Yang, Qing Xu, Xingya Li, Jinxing Lou, Jianchun Duan, Jiachen Xu, Zhuqing Liu, Yong Xia, Zhicai Lin, Linlin Li, Dan Sun, Jiaguo Li, Tao Liu, Jun Guo, Wenfeng Xu, Weimin Zhu, Yi Liu, Boyang Sun, Jia Zhong, Lijie Rong, Qijun Qian, Chenqi Xu, Jie Wang   +23 more
wiley   +1 more source

CAR T cell engineering impacts antigen-independent activation and co-inhibition

Molecular Therapy: Methods & Clinical Development
Viral vectors have successfully modified T cells to express chimeric antigen receptors (CARs), leading to clinical approvals. However, their high cost and regulatory challenges hinder rapid clinical translation. Here, we demonstrate that our lentivirally
Christoph Schultheiß, Simon Stücheli, Brenda Besemer, Maja Kadel, Paul Schmidt-Barbo, Andreas Zingg, Natascha Franz, Sarah Adamo, Frank Stenner, Claudia Fischer, Heinz Läubli, Mascha Binder   +11 more
doaj   +1 more source

A High-Capacity Adenoviral Hybrid Vector System Utilizing the Hyperactive Sleeping Beauty Transposase SB100X for Enhanced Integration

Molecular Therapy: Nucleic Acids, 2016
For efficient delivery of required genetic elements we utilized high-capacity adenoviral vectors in the past allowing high transgene capacities of up to 36 kb.
Philip Boehme, Wenli Zhang, Manish Solanki, Eric Ehrke-Schulz, Anja Ehrhardt   +4 more
doaj   +1 more source

Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion [PDF]

, 2018
In a recent past, transposable elements (TEs) were referred to as selfish genetic components only capable of copying themselves with the aim of increasing the odds of being inherited.
A Bolotin, A Burt, A Hammond, AP Lorenzetti, B McClintock, BA Castilho, C Feschotte, C Feschotte, C Feschotte, C Kettlun, C Lu, C Ye, CN Hancock, DBT Cox, DJ Garfinkel, F Palazzoli, FA Ran, FJM Mojica, G Feng, G Mai, G Yang, H Kuang, H Mao, HT Dong, IK Jordan, J Hou, J Li, J Paszkowski, JA Doudna, JB Owens, JD Hollister, JE DiCarlo, JI Qüesta, JK Baillie, JM Casacuberta, K Kikuchi, K Naito, K Naito, K Shirasawa, KD Kim, KM Esvelt, KS Makarova, L Bortesi, L Cong, LC Li, LM Vaschetto, Luis María Vaschetto, M Cowley, M Jinek, M Momose, M Patel, M Szabo, M Turner, N Buchon, N Jiang, N Jiang, N Jiang, Q Zhang, R Barrangou, R Jansen, RM Walsh, SD Molyneux, SR Wessler, SR Yant, T Mourier, T Sakuma, T Singer, VM Gantz, W Matsunaga, WC Lai, X Li, X Li, X Lin, X Shan, Y Ishino, Y Yan, ZN Adelman   +76 more
core   +1 more source

Paternal Circadian Disruption Impairs Offspring Cognition via Sperm microRNAs

Advanced Science, EarlyView.
Paternal circadian disruption remodels the sperm small RNA payload, elevating miR‐92a‐3p/miR‐25‐3p levels and perturbing early embryonic gene regulatory programs. Microinjection experiments and single‐embryo transcriptomics reveal sex‐specific developmental vulnerabilities, ultimately impairing offspring hippocampal synaptic plasticity and cognition ...
Kexin Zou, Sisi Luo, Binliang Tang, Zhiqiang Liu, Yuchuan Zhou, Yicong Meng, Zhengmu Wu, Zheng Sun, Weihui Shi, Jianzhong Sheng, Chuanjin Yu, Zhenhua Li, Siyi Wei, Mo Zhang, Yujie Luo, Jing Yan, Xueyun Qin, Jiahang Mo, Chengliang Zhou, Jinsong Li, Hefeng Huang, Yifu Ding, Guolian Ding   +22 more
wiley   +1 more source

Hybrid adeno-associated viral vectors utilizing transposase-mediated somatic integration for stable transgene expression in human cells.

PLoS ONE, 2013
Recombinant adeno-associated viral (AAV) vectors have been shown to be one of the most promising vectors for therapeutic gene delivery because they can induce efficient and long-term transduction in non-dividing cells with negligible side-effects ...
Wenli Zhang, Manish Solanki, Nadine Müther, Melanie Ebel, Jichang Wang, Chuanbo Sun, Zsuzsanna Izsvak, Anja Ehrhardt   +7 more
doaj   +1 more source

Structure, Activity, and Function of SETMAR Protein Lysine Methyltransferase

Life, 2021
SETMAR is a protein lysine methyltransferase that is involved in several DNA processes, including DNA repair via the non-homologous end joining (NHEJ) pathway, regulation of gene expression, illegitimate DNA integration, and DNA decatenation.
Michael Tellier
doaj   +1 more source