Results 61 to 70 of about 68,477 (364)
Regulation of mariner transposition: the peculiar case of Mos1. [PDF]
PLoS ONE, 2012 BACKGROUND: Mariner elements represent the most successful family of autonomous DNA transposons, being present in various plant and animal genomes, including humans.
Jérôme Jaillet +4 more
doaj +1 more source
Subunit Interactions in the mariner Transposase [PDF]
Genetics, 1996 Abstract We have studied the Mos1 transposase encoded by the transposable element mariner. This transposase is a member of the “D,D(35)E” superfamily of proteins exhibiting the motif D,D(34)D. It is not known whether this transposase, or other eukaryote transposases manifesting the D,D(35)E domain, functions in a multimeric form ...
A R, Lohe, D T, Sullivan, D L, Hartl
openaire +2 more sources
DDE transposases: Structural similarity and diversity [PDF]
Advanced Drug Delivery Reviews, 2010 DNA transposons are mobile DNA elements that can move from one DNA molecule to another and thereby deliver genetic information into human chromosomes in order to confer a new function or replace a defective gene. This process requires a transposase enzyme. During transposition DD[E/D]-transposases undergo a series of conformational changes.
Irina V, Nesmelova, Perry B, Hackett
openaire +2 more sources
Molecular Therapy: Nucleic Acids, 2016 The Sleeping Beauty (SB) transposon system has been shown to enable long-term gene expression by integrating new sequences into host cell chromosomes. We found that the recently reported SB100x hyperactive transposase conferred a surprisingly high level ...
Kelly M Podetz-Pedersen +4 more
doaj +1 more source
The folding of the specific DNA recognition subdomain of the sleeping beauty transposase is temperature-dependent and is required for its binding to the transposon DNA. [PDF]
PLoS ONE, 2014 The reaction of DNA transposition begins when the transposase enzyme binds to the transposon DNA. Sleeping Beauty is a member of the mariner family of DNA transposons.
Gage O Leighton +5 more
doaj +1 more source
Microorganisms, 2019 During an oxidative stress-response assay on a putative Dps-like gene-disrupted Δdgeo_0257 mutant strain of radiation-resistant bacterium Deinococcus geothermalis, a non-pigmented colony was observed among the normal reddish color colonies. This non-
Chanjae Lee +4 more
doaj +1 more source
Structural insight into Tn3 family transposition mechanism
Nature Communications, 2022 Here the structure of transposase from Tn3 family is reported in apo form and bound to the transposon ends. The activation of the transposase induces metamorphic refolding of the catalytic domain suggesting the family-specific regulation mechanism.
Alexander V. Shkumatov +5 more
doaj +1 more source
A highly soluble Sleeping Beauty transposase improves control of gene insertion
Nature Biotechnology, 2019 The Sleeping Beauty (SB) transposon system is an efficient non-viral gene transfer tool in mammalian cells, but its broad use has been hampered by uncontrolled transposase gene activity from DNA vectors, posing a risk of genome instability, and by the ...
Irma Querques +11 more
semanticscholar +1 more source
Tn5 transposase and tagmentation procedures for massively scaled sequencing projects
Genome Research, 2014 Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming.
S. Picelli +5 more
semanticscholar +1 more source
The Role of Vertical and Horizontal Transfer in the Evolutionary Dynamics of PIF-Like Transposable Elements in Triticeae. [PDF]
PLoS ONE, 2015 PIF-like transposable elements are members of the PIF/Harbinger superfamily of DNA transposons found in the genomes of many plants, animals, and fungi.
Dragomira N Markova +1 more
doaj +1 more source