Functional characterization of the Bari1 transposition system. [PDF]
The transposons of the Bari family are mobile genetic elements widespread in the Drosophila genus. However, despite a broad diffusion, virtually no information is available on the mechanisms underlying their mobility.
Antonio Palazzo +6 more
doaj +1 more source
piggyBac transposase tools for genome engineering [PDF]
Xianghong Li +12 more
openalex +2 more sources
DNA-binding activity and subunit interaction of the mariner transposase [PDF]
Mos1 is a member of the mariner/Tc1 family of transposable elements originally identified in Drosophila mauritiana. It has 28 bp terminal inverted repeats and like other elements of this type it transposes by a cut and paste mechanism, inserts at TA ...
Finnegan, D J, Dawson, A, Zhang, Lei
core +1 more source
Identification and characterization of domesticated bacterial transposases [PDF]
ABSTRACT Selfish genetic elements (SGEs), such as insertion sequences (ISs) and transposons are found in most genomes. Transposons are usually identifiable by their high copy number within genomes. In contrast REP associated tyrosine transposases (RAYTs), a recently described class of bacterial transposase, are ...
Bertels, Frederic +2 more
openaire +3 more sources
Precise targeted integration by a chimaeric transposase zinc-finger fusion protein [PDF]
Transposons of the Tc1/mariner family have been used to integrate foreign DNA stably into the genome of a large variety of different cell types and organisms.
Amy L. Bednarz +5 more
core +1 more source
Optimization of the piggyBac transposon using mRNA and insulators: toward a more reliable gene delivery system. [PDF]
Integrating and expressing stably a transgene into the cellular genome remain major challenges for gene-based therapies and for bioproduction purposes.
Solenne Bire +5 more
doaj +1 more source
RNA interference is responsible for reduction of transgene expression after Sleeping Beauty transposase mediated somatic integration. [PDF]
Integrating non-viral vectors based on transposable elements are widely used for genetically engineering mammalian cells in functional genomics and therapeutic gene transfer.
Anja Ehrhardt +7 more
core +1 more source
Beetle luciferase, a mono-oxygenase within the AMP-binding superfamily, is synthesized by bioluminescent beetles in concentrated levels within specialised cells clustering in the abdominal light organs.
John C. DAY
doaj +1 more source
Crystallization of a Mos1 transposase-inverted-repeat DNA complex: biochemical and preliminary crystallographic analyses [PDF]
A complex formed between Mos1 transposase and its inverted-repeat DNA has been crystallized. The crystals diffract to 3.25 angstrom resolution and exhibit monoclinic (P2(1)) symmetry, with unit-cell parameters a = 120.8, b = 85.1, c = 131.6 angstrom ...
Richardson, Julia M.; id_orcid +2 more
core +1 more source
Marine sediments harbor a vast amount of Earth’s microbial biomass, yet little is understood regarding how cells subsist in this low-energy, presumably slow-growth environment.
Ian M. Rambo +2 more
doaj +1 more source

