Results 51 to 60 of about 77,105 (268)

Tn5 transposase‐assisted transformation of indica rice [PDF]

The Plant Journal, 2011
SummaryHere, we describe experiments on Tn5 transposase‐assisted transformation of indica rice. Transposomes were formed in vitro as a result of hyperactive Tn5 transposase complexing with a transposon that contained a 19‐bp tetracycline operator (tetO) sequence. To form modified projectiles for transformation, the Tn10‐derived prokaryotic tetracycline
Jun, Wu, Hongwei, Du, Xuewei, Liao, Yu, Zhao, Liguan, Li, Liuyan, Yang   +5 more
openaire   +2 more sources

An abundant evolutionarily conserved CSB-PiggyBac fusion protein expressed in Cockayne syndrome. [PDF]

PLoS Genetics, 2008
Cockayne syndrome (CS) is a devastating progeria most often caused by mutations in the CSB gene encoding a SWI/SNF family chromatin remodeling protein. Although all CSB mutations that cause CS are recessive, the complete absence of CSB protein does not ...
John C Newman, Arnold D Bailey, Hua-Ying Fan, Thomas Pavelitz, Alan M Weiner   +4 more
doaj   +1 more source

Transposase-mediated gene modulation in the placenta [PDF]

Placenta, 2017
The placenta is an organ vital to fetal development as well as the maintenance of a healthy pregnancy and plays a crucial role in developmental programming of the fetus. The mechanisms that link intrauterine milieu, fetal health and disease development later in life are poorly understood. Placenta-specific gene modulation, both by generating transgenic
Marlee Elston, Johann Urschitz
openaire   +2 more sources

Phylogenomic analysis of lactobacillus curvatus reveals two lineages distinguished by genes for fermenting plant-derived carbohydrates [PDF]

, 2018
Lactobacillus curvatus is a lactic acid bacterium encountered in many different types of fermented food (meat, seafood, vegetables, and cereals). Although this species plays an important role in the preservation of these foods, few attempts have been ...
Chaillou, Stéphane, Champomier-Vergès, Marie-Christine, Coeuret, Gwendoline, Raya, Raul Ricardo, Teran, Lucrecia Cecilia, Zagorec, Monique   +5 more
core   +3 more sources

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

High-throughput chromatin accessibility profiling at single-cell resolution. [PDF]

, 2018
Here we develop a high-throughput single-cell ATAC-seq (assay for transposition of accessible chromatin) method to measure physical access to DNA in whole cells.
Bostick, Magnolia, Brower, Kara, Farmer, Andrew, Fordyce, Polly, Greenleaf, William, Klemm, Sandy, Linnarsson, Sten, Mann, Ishminder, Mezger, Anja, Mir, Alain   +9 more
core   +3 more sources

Improved genome sequencing using an engineered transposase [PDF]

BMC Biotechnology, 2017
Next-generation sequencing (NGS) has transformed genomic research by reducing turnaround time and cost. However, no major breakthrough has been made in the upstream library preparation methods until the transposase-based Nextera method was invented. Nextera combines DNA fragmentation and barcoding in a single tube reaction and therefore enables a very ...
Kia, Amirali, Gloeckner, Christian, Osothprarop, Trina, Gormley, Niall, Bomati, Erin, Stephenson, Michelle, Goryshin, Igor, He, Molly Min   +7 more
openaire   +2 more sources

Poly(lactic‐co‐glycolic acid) Nanoparticles for IL‐12 Self‐Amplifying RNA Delivery in Glioblastoma Models

Advanced Functional Materials, EarlyView.
xxxx. ABSTRACT Glioblastoma (GBM) remains one of the most lethal brain cancers, with median survival rarely exceeding 15 months after diagnosis. Interleukin‐12 (IL‐12) is a potent immunostimulatory cytokine capable of reshaping the tumor microenvironment (TME), yet its clinical translation is hindered by systemic toxicity and short half‐life. RNA‐based
Fatima Hameedat, Johan Sebastian Lopez‐Parra, Willem M. H. Hoogaars, Emma Dijkstra, Cynthia Huang, Irafasha C. Casmil, Anna K. Blakney, Peter Olinga, Barbara Rothen‐Rutishauser, Hélder A. Santos, Alke Petri‐Fink, Flávia Sousa   +11 more
wiley   +1 more source

piggyBac transposase tools for genome engineering [PDF]

Proceedings of the National Academy of Sciences, 2013
Significance DNA transposons that translocate by excision from a donor site and insertion into a target site are often used for genome engineering by insertional mutagenesis and transgenesis. The piggyBac element is especially useful because it can excise precisely from an insertion site, restoring the ...
Xianghong, Li, Erin R, Burnight, Ashley L, Cooney, Nirav, Malani, Troy, Brady, Jeffry D, Sander, Janice, Staber, Sarah J, Wheelan, J Keith, Joung, Paul B, McCray, Frederic D, Bushman, Patrick L, Sinn, Nancy L, Craig   +12 more
openaire   +2 more sources

ETV2 Mediated Differentiation of Human Pluripotent Stem Cells Results in Functional Endothelial Cells for Engineering Advanced Vascularized Microphysiological Models

Advanced Healthcare Materials, EarlyView.
A robust method to generate functional human iPSC‐derived endothelial cells using inducible ETV2 expression. These cells self‐organize into stable, lumenized microvascular networks within microfluidic chips, surpassing conventional differentiation methods.
Shun Zhang, Zhengpeng Wan, Lei Wang, Caihong Wu, Junkai Zhang, Sarah Spitz, Xun Wang, Marie A. Floryan, Mark F. Coughlin, Francesca M. Pramotton, Liling Xu, Ron Weiss, Roger D. Kamm   +12 more
wiley   +1 more source