Wellcome Open Research, 2021 Background Genetic systems have been developed for Chlamydia but the extremely low transformation frequency remains a significant bottleneck. Our goal is to develop a self-replicating transposon delivery vector for C.
Rachel J. Skilton +4 more
doaj +1 more source
Mechanism and regulation of P element transposition [PDF]
Open Biology, 2020 P elements were first discovered in the fruit fly Drosophila melanogaster as the causative agents of a syndrome of aberrant genetic traits called hybrid dysgenesis.
George E. Ghanim +2 more
doaj +1 more source
Molecular Therapy: Nucleic AcidsThe Sleeping Beauty (SB) transposon system is a useful tool for genetic applications, including gene therapy. We discovered a hyperactive variant of the SB100X transposase, called SB200X.
Matthias Thomas Ochmann +5 more
doaj +1 more source
Transcriptionally regulated and nontoxic delivery of the hyperactive Sleeping Beauty Transposase
Molecular Therapy: Methods & Clinical Development, 2016 The Sleeping Beauty (SB) transposase and, in particular, its hyperactive variant SB100X raises increasing interest for gene therapy application, including genome modification and, more recently, induced pluripotent stem cells (iPS) reprogramming.
Fabienne Cocchiarella +6 more
doaj +1 more source
ALS mutant FUS proteins are recruited into stress granules in induced pluripotent stem cells- derived motoneurons [PDF]
, 2015 Patient-derived induced Pluripotent Stem Cells (iPSCs) provide an opportunity to study human diseases mainly in those cases where no suitable model systems are available.
Bozzoni, Irene +9 more
core +4 more sources
Increasing transposase abundance with ocean depth correlates with a particle-associated lifestyle
mSystemsTransposases are mobile genetic elements that move within and between genomes, promoting genomic plasticity in microorganisms. In marine microbial communities, the abundance of transposases increases with depth, but the reasons behind this trend remain ...
Juntao Zhong +5 more
doaj +1 more source
Recurrent co-domestication of PIF/Harbinger transposable element proteins in insects
Mobile DNA, 2022 Background Transposable elements (TEs) are selfish DNA sequences capable of moving and amplifying at the expense of host cells. Despite this, an increasing number of studies have revealed that TE proteins are important contributors to the emergence of ...
Dragomira N. Markova +4 more
doaj +1 more source
Structure and evolution of chlorate reduction composite transposons. [PDF]
, 2013 UnlabelledThe genes for chlorate reduction in six bacterial strains were analyzed in order to gain insight into the metabolism. A newly isolated chlorate-reducing bacterium (Shewanella algae ACDC) and three previously isolated strains (Ideonella ...
Clark, Iain C +3 more
core +2 more sources
Subunit Interactions in the mariner Transposase [PDF]
Genetics, 1996 Abstract We have studied the Mos1 transposase encoded by the transposable element mariner. This transposase is a member of the “D,D(35)E” superfamily of proteins exhibiting the motif D,D(34)D. It is not known whether this transposase, or other eukaryote transposases manifesting the D,D(35)E domain, functions in a multimeric form ...
A R, Lohe, D T, Sullivan, D L, Hartl
openaire +2 more sources
Insights into a Novel blaKPC-2-Encoding IncP-6 Plasmid Reveal Carbapenem-Resistance Circulation in Several Enterobacteriaceae Species from Wastewater and a Hospital Source in Spain [PDF]
, 2017 Untreated wastewater, particularly from hospitals and other healthcare facilities, is considered to be a reservoir for multidrug-resistant bacteria.
Cantón, Rafael +8 more
core +1 more source