Results 41 to 50 of about 68,477 (364)
Targeted genetic screening in bacteria with a Cas12k-guided transposase.
Cell Reports, 2021 Microbes employ sophisticated cellular networks encoded by complex genomes to rapidly adapt to changing environments. High-throughput genome engineering methods are valuable tools for functionally profiling genotype-phenotype relationships and ...
Weizhong Chen +11 more
semanticscholar +1 more source
Emerging Infectious Diseases, 2023 Incidence of Streptococcus dysgalactiae subspecies equisimilis (SDSE) bacteremia is increasing in the Kyoto-Shiga region of Japan. We retrospectively analyzed clinical features of SDSE bacteremia and conducted comparative genomic analyses of isolates ...
Koh Shinohara +8 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2022 In recent years, mammalian cells have become the primary host cells for the production of recombinant therapeutic proteins (RTPs). Despite that the expression of RTPs in mammalian cells can be improved by directly optimizing or engineering the expression
Mian Wei +4 more
doaj +1 more source
Recurrent evolution of vertebrate transcription factors by transposase capture
Science, 2020 A recipe for new genes Most lineages contain evolutionarily novel genes, but their origin is not always clear. Cosby et al. investigated the origin of families of lineage-specific vertebrate genes (see the Perspective by Wacholder and Carvunis).
Rachel L. Cosby +6 more
semanticscholar +1 more source
Structural basis of seamless excision and specific targeting by piggyBac transposase
Nature Communications, 2020 The piggyBac DNA transposon is used widely in genome engineering applications. Unlike other transposons, its excision site can be precisely repaired without leaving footprints and it integrates specifically at TTAA tetranucleotides.
Qiujia Chen +5 more
semanticscholar +1 more source
Gene, 2022 Transposable elements are widespread in all living organisms. In addition to self-reproduction, they are a major source of genetic variation that drives genome evolution but our knowledge of the functions of human genes derived from transposases is limited.
Kolacsek, Orsolya +10 more
openaire +3 more sources
Bacterial transposases and retroviral integrases [PDF]
Molecular Microbiology, 1995 SummaryTransposable genetic elements have adopted two major strategies for their displacement from one site to another within and between genomes. One involves passage through an RNA intermediate prior to synthesis of a DNA copy while the other is limited uniquely to DNA intermediates.
P, Polard, M, Chandler
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Insertion Specificity of the hATx-6 Transposase of Hydra magnipapillata
Frontiers in Molecular Biosciences, 2021 Transposable elements (TE) are mobile genetic elements, present in all domains of life. They commonly encode a single transposase enzyme, that performs the excision and reintegration reactions, and these enzymes have been used in mutagenesis and creation
Paul Riggs +5 more
doaj +1 more source
Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
bioRxiv, 2020 Tn5-mediated transposition of double-strand DNA has been widely utilized in various high-throughput sequencing applications. Here, we report that the Tn5 transposase is also capable of direct tagmentation of RNA/DNA hybrids in vitro.
Bo Lu +6 more
semanticscholar +1 more source
Isolation and characterization of Tn7 transposase gain-of-function mutants: a model for transposase activation [PDF]
The EMBO Journal, 2000 Tn7 transposition has been hypothesized to require a heteromeric transposase formed by two Tn7-encoded proteins, TnsA and TnsB, and accessory proteins that activate the transposase when they are associated with an appropriate target DNA. This study investigates the mechanism of Tn7 transposase activation by isolation and analysis of transposase gain-of-
F, Lu, N L, Craig
openaire +2 more sources