Results 21 to 30 of about 68,477 (364)
, 2013 Transposase is a transposable element-encoded recombinase protein that is responsible for the element’s movement or transposition. The transposase recognizes specific sequences in the transposon and performs a specific recombination reaction called ...
Z. Izsvák +5 more
core +2 more sources
, 2017 Transposase is a transposable element-encoded recombinase protein that is responsible for the element’s movement or transposition. The transposase recognizes specific sequences in the transposon and performs a specific recombination reaction called ...
Z. Izsvák +3 more
core +2 more sources
Partial Sequencing of IS1216V Transposase Gene of Staphylococcus Aureus Isolated from Food Samples
مجله كليه طب الكندي, 2019 Background: Insertion sequence is a short DNA sequence encode for proteins implicated in the transposition activity. Transposase catalyzes the enzymatic reaction allowing the insertion sequence to +9*lo2 move. ;qqa;.
Ibtesam G. Auda
doaj +3 more sources
Transcriptionally regulated and nontoxic delivery of the hyperactive Sleeping Beauty Transposase [PDF]
Molecular Therapy: Methods & Clinical Development, 2016 The Sleeping Beauty (SB) transposase and, in particular, its hyperactive variant SB100X raises increasing interest for gene therapy application, including genome modification and, more recently, induced pluripotent stem cells (iPS) reprogramming.
Fabienne Cocchiarella +6 more
doaj +2 more sources
Molecular Therapy: Nucleic Acids, 2016 For efficient delivery of required genetic elements we utilized high-capacity adenoviral vectors in the past allowing high transgene capacities of up to 36 kb.
Philip Boehme +4 more
doaj +2 more sources
A hyperactive
BMC Research Notes, 2010 Background Transposons are useful molecular tools for transgenesis. The 'sleeping beauty' transposon is a synthetic member of the Tc1/mariner transposon family. Davidson et al. (2003) previously described a vector for zebrafish transgenesis consisting of
Lardelli Michael, Newman Morgan
doaj +2 more sources
Repression of IS200 transposase synthesis by RNA secondary structures [PDF]
Nucleic Acids Research, 1999 The IS200 transposase, a 16 kDa polypeptide encoded by the single open reading frame (ORF) of the insertion element, has been identified using an expression system based on T7 RNA polymerase. In wild-type IS200, two sets of internal inverted repeats that
Carmen R Beuzon +2 more
exaly +2 more sources
Improved genome sequencing using an engineered transposase [PDF]
BMC Biotechnology, 2017 Background Next-generation sequencing (NGS) has transformed genomic research by reducing turnaround time and cost. However, no major breakthrough has been made in the upstream library preparation methods until the transposase-based ...
Stephenson, Michelle +15 more
core +2 more sources
Transposase-Transposase Interactions in MOS1 Complexes: A Biochemical Approach.
Journal of Molecular Biology, 2011 International audienceTransposases are proteins that have assumed the mobility of class II transposable elements. In order to map the interfaces involved in transposase transposase interactions, we have taken advantage of 12 transposase mutants that ...
Auge-Gouillou, Corinne +5 more
core +4 more sources
Molecular Therapy: Nucleic Acids, 2018 Continuous innovation of revolutionizing genome engineering technologies calls for an intensified focus on new delivery technologies that not only match the inventiveness of genome editors but also enable the combination of potent delivery and time ...
Kristian Alsbjerg Skipper +5 more
doaj +2 more sources