Results 11 to 20 of about 68,477 (364)
Biochemical analysis of nucleosome targeting by Tn5 transposase [PDF]
Open Biology, 2019 Tn5 transposase is a bacterial enzyme that integrates a DNA fragment into genomic DNA, and is used as a tool for detecting nucleosome-free regions of genomic DNA in eukaryotes.
Shoko Sato +2 more
exaly +3 more sources
Tn5 DNA Transposase in Multi-Omics Research
Methods and Protocols, 2023 Tn5 transposase use in biotechnology has substantially advanced the sequencing applications of genome-wide analysis of cells. This is mainly due to the ability of Tn5 transposase to efficiently transpose DNA essentially randomly into any target DNA ...
Ekaterina Zubkova +2 more
exaly +4 more sources
Molecular Cell, 2021 SUMMARY Helitrons are widespread eukaryotic DNA transposons that have significantly contributed to genome variability and evolution, in part because of their distinctive, replicative rolling-circle mechanism, which often mobilizes adjacent genes ...
Dalibor Košek +2 more
exaly +4 more sources
Tn5 Transposase Applied in Genomics Research [PDF]
International Journal of Molecular Sciences, 2020 The development of high-throughput sequencing (next-generation sequencing technology (NGS)) and the continuous increase in experimental throughput require the upstream sample processing steps of NGS to be as simple as possible to improve the efficiency ...
Kairang Jin +5 more
core +4 more sources
A Hyperactive Transposase Promotes Persistent Gene Transfer of a piggyBac DNA Transposon
Molecular Therapy - Nucleic Acids, 2012 Nonviral vector systems are used increasingly in gene targeting and gene transfer applications. The piggyBac transposon represents an alternative integrating vector for in vivo gene transfer. We hypothesized that this system could achieve persistent gene
Janice M Staber +2 more
exaly +4 more sources
A hyperactive piggyBac transposase for mammalian applications [PDF]
Proceedings of the National Academy of Sciences, 2011 DNA transposons have been widely used for transgenesis and insertional mutagenesis in various organisms. Among the transposons active in mammalian cells, the moth-derived transposon piggyBac is most promising with its
Meng Amy Li +4 more
core +4 more sources
Epigenetic Application of ATAC-Seq Based on Tn5 Transposase Purification Technology [PDF]
Genetics Research, 2022 Background. Assays of transposase accessible chromatin sequencing (ATAC-seq) is an efficient assay to investigate chromatin accessibility, which depends on the activity of a robust Tn5 transposase to fragment the genome while cutting in the sequencing ...
Wangchun Li +8 more
doaj +2 more sources
Active recombinant Tol2 transposase for gene transfer and gene discovery applications [PDF]
Mobile DNA, 2016 The revolutionary concept of “jumping genes” was conceived by McClintock in the late 1940s while studying the Activator/Dissociation (Ac/Ds) system in maize.
Jun Ni +6 more
openalex +2 more sources
Evidence of horizontal gene transfer by transposase gene analyses in Fervidobacterium species. [PDF]
PLoS ONE, 2017 Horizontal Gene Transfer (HGT) plays an important role in the physiology and evolution of microorganisms above all thermophilic prokaryotes. Some members of the Phylum Thermotogae (i.e., Thermotoga spp.) have been reported to present genomes constituted ...
Alba Cuecas +2 more
doaj +2 more sources
, 2023 <p><a href="https://www.creative-enzymes.com/product/tn5-transposase_8479.html">Tn5 Transposase</a> is an enzyme that binds to the end of a transposon and catalyzes its movement to another part of the genome by a cut-and-paste mechanism
Lisa Clara
core +2 more sources