Results 11 to 20 of about 77,105 (268)
Functional indications for transposase domestications – Characterization of the human piggyBac transposase derived (PGBD) activities [PDF]
Gene, 2022 Transposable elements are widespread in all living organisms. In addition to self-reproduction, they are a major source of genetic variation that drives genome evolution but our knowledge of the functions of human genes derived from transposases is limited.
Kolacsek, Orsolya +10 more
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Bacterial transposases and retroviral integrases [PDF]
Molecular Microbiology, 1995 SummaryTransposable genetic elements have adopted two major strategies for their displacement from one site to another within and between genomes. One involves passage through an RNA intermediate prior to synthesis of a DNA copy while the other is limited uniquely to DNA intermediates.
P, Polard, M, Chandler
openaire +2 more sources
Tn5 Transposase Applied in Genomics Research [PDF]
International Journal of Molecular Sciences, 2020 The development of high-throughput sequencing (next-generation sequencing technology (NGS)) and the continuous increase in experimental throughput require the upstream sample processing steps of NGS to be as simple as possible to improve the efficiency of the entire NGS process.
Li, Niannian +5 more
openaire +2 more sources
Tn 5 Transposase Active Site Mutants [PDF]
Journal of Biological Chemistry, 2002 Tn5 transposase (Tnp) is a 53.3-kDa protein that is encoded by and facilitates movement of transposon Tn5. Tnp monomers contain a single active site that is responsible for catalyzing a series of four DNA breaking/joining reactions at one transposon end.
Todd A, Naumann, William S, Reznikoff
openaire +2 more sources
Escherichia coli EHEC Germany outbreak preliminary functional annotation using BG7 system [PDF]
, 2011 We have annotated the European outbreak E. coli EHEC genome sequenced by BGI (6-2-2011) and assembled with MIRA by Nick Loman (6-2-2011 ). Our system BG7, Bacterial Genome annotation of Era7 Bioinformatics, predicts ORFs and annotates them based on ...
Eduardo Pareja +4 more
core +2 more sources
Optimization of the piggyBac transposon using mRNA and insulators: toward a more reliable gene delivery system. [PDF]
PLoS ONE, 2013 Integrating and expressing stably a transgene into the cellular genome remain major challenges for gene-based therapies and for bioproduction purposes.
Solenne Bire +5 more
doaj +1 more source
Open-source genomic analysis of Shiga-toxin–producing E. coli O104:H4 [PDF]
, 2011 An outbreak caused by Shiga-toxin–producing Escherichia coli O104:H4 occurred in Germany in May and June of 2011, with more than 3000 persons infected. Here, we report a cluster of cases associated with a single family and describe an open-source genomic
Antoine Danchin +39 more
core +1 more source
European Journal of Entomology, 2005 Beetle luciferase, a mono-oxygenase within the AMP-binding superfamily, is synthesized by bioluminescent beetles in concentrated levels within specialised cells clustering in the abdominal light organs.
John C. DAY
doaj +1 more source
Chimeric piggyBac transposases for genomic targeting in human cells. [PDF]
, 2012 Integrating vectors such as viruses and transposons insert transgenes semi-randomly and can potentially disrupt or deregulate genes. For these techniques to be of therapeutic value, a method for controlling the precise location of insertion is required ...
Belcaid, Mahdi +9 more
core +5 more sources
Frontiers in Microbiology, 2019 Marine sediments harbor a vast amount of Earth’s microbial biomass, yet little is understood regarding how cells subsist in this low-energy, presumably slow-growth environment.
Ian M. Rambo +2 more
doaj +1 more source