Results 1 to 10 of about 121,903 (271)
The autoregulation of a eukaryotic DNA transposon [PDF]
eLife, 2013 How do DNA transposons live in harmony with their hosts? Bacteria provide the only documented mechanisms for autoregulation, but these are incompatible with eukaryotic cell biology.
Corentin Claeys Bouuaert +4 more
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Activity of the mammalian DNA transposon piggyBat from Myotis lucifugus is restricted by its own transposon ends [PDF]
Nature CommunicationsMembers of the piggyBac superfamily of DNA transposons are widely distributed in host genomes ranging from insects to mammals. The human genome has retained five piggyBac-derived genes as domesticated elements although they are no longer mobile. Here, we
Alison B. Hickman +13 more
doaj +2 more sources
Microbiology Spectrum, 2022 Bacteriophage-mediated transduction of bacterial DNA is a major route of horizontal gene transfer in the human pathogen, Staphylococcus aureus. Transduction involves the packaging of bacterial DNA by viruses and enables the transmission of virulence and ...
Janine Zara Bowring +5 more
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Unbiased profiling of CRISPR RNA-guided transposition products by long-read sequencing
Mobile DNA, 2021 Bacterial transposons propagate through either non-replicative (cut-and-paste) or replicative (copy-and-paste) pathways, depending on how the mobile element is excised from its donor source. In the well-characterized E. coli transposon Tn7, a heteromeric
Phuc Leo H. Vo +3 more
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Campylobacter jejuni genes Cj1492c and Cj1507c are involved in host cell adhesion and invasion [PDF]
, 2020 Background Campylobacter jejuni (C. jejuni) has been assigned as an important food-borne pathogen for human health but many pathogenicity factors of C. jejuni and human host cell responses related to the infection have not yet been adequately clarified.
Alter, Thomas +4 more
core +1 more source
Rapid and Simple Amplification of Genomic DNA Sequences Flanking Transposon
Microbiology Indonesia, 2010 A rapid and simple method to amplify genomic DNA sequences flanking mini-Tn5 transposon insertion was developed. This technique can be used to determine the location and orientation of the transposon insertion within genomic DNA of the bacteria. Based on
ARIS TRI WAHYUDI
doaj +1 more source
Transcription activator like effector (TALE)-directed piggyBac transposition in human cells. [PDF]
, 2013 Insertional therapies have shown great potential for combating genetic disease and safer methods would undoubtedly broaden the variety of possible illness that can be treated. A major challenge that remains is reducing the risk of insertional mutagenesis
Bhakta, Mital S +6 more
core +3 more sources
Efficient Sleeping Beauty DNA Transposition From DNA Minicircles
Molecular Therapy: Nucleic Acids, 2013 DNA transposon-based vectors have emerged as new potential delivery tools in therapeutic gene transfer. Such vectors are now showing promise in hematopoietic stem cells and primary human T cells, and clinical trials with transposon-engineered cells are ...
Nynne Sharma +5 more
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Chimeric piggyBac transposases for genomic targeting in human cells. [PDF]
, 2012 Integrating vectors such as viruses and transposons insert transgenes semi-randomly and can potentially disrupt or deregulate genes. For these techniques to be of therapeutic value, a method for controlling the precise location of insertion is required ...
Belcaid, Mahdi +9 more
core +5 more sources
Mapping transposon insertion sites by touchdown PCR and hybrid degenerate primers
BioTechniques, 2005 A novel mapping method based on touchdown PCR was developed for identifying a transposon insertion site in genomic DNA using a hybrid consensus-degenerate primer in combination with a specific primer that anneals to the transposon.
Julio Levano-Garcia +2 more
doaj +1 more source