Results 31 to 40 of about 121,903 (271)
Molecular Therapy: Nucleic Acids, 2016 The Sleeping Beauty (SB) transposon system has been shown to enable long-term gene expression by integrating new sequences into host cell chromosomes. We found that the recently reported SB100x hyperactive transposase conferred a surprisingly high level ...
Kelly M Podetz-Pedersen +4 more
doaj +1 more source
Germ granule dysfunction is a hallmark and mirror of Piwi mutant sterility
Nature Communications, 2021 Piwi deficiency results in sterility and is associated with transposon expression and genomic instability. Here the authors show that sterility of C. elegans Piwi prg-1 mutant is not associated with transposon-induced DNA damage but is associated with ...
Maya Spichal +5 more
doaj +1 more source
Molecular Plant-Microbe Interactions, 2001 A transposon mutagenesis system for Clavibacter michiganensis subsp. michiganensis was developed based on antibiotic resistance transposons that were derived from the insertion element IS1409 from Arthrobacter sp. strain TM1 NCIB12013. As a prerequisite,
Oliver Kirchner +4 more
doaj +1 more source
Insight into the molecular mechanism of the transposon-encoded type I-F CRISPR-Cas system
Journal of Genetic Engineering and Biotechnology, 2023 CRISPR-Cas9 is a popular gene-editing tool that allows researchers to introduce double-strand breaks to edit parts of the genome. CRISPR-Cas9 system is used more than other gene-editing tools because it is simple and easy to customize.
Amnah Alalmaie +2 more
doaj +1 more source
Introduction of Plant Transposon Annotation for Beginners
Biology, 2023 Transposons are mobile DNA sequences that contribute large fractions of many plant genomes. They provide exclusive resources for tracking gene and genome evolution and for developing molecular tools for basic and applied research.
Dongying Gao
doaj +1 more source
Gene transfer in the GI tract and oral cavity [PDF]
, 2000 Gene transfer is important in spreading antibiotic resistance and other traits such as virulence factors. In this review the molecular mechanisms of gene transfer are outlined and the biological consequences of bacterial gene transfer in the GI tract and
Mullany, P
core +1 more source
Life Science Alliance, 2018 Suppression of transposons by the Piwi-interacting RNA biogenesis factor Vasa in the supporting nurse cells is essential to prevent their accumulation in the developing oocyte, ensuring proper Drosophila embryonic development.
Zeljko Durdevic +2 more
doaj +1 more source
mSystems, 2023 The massive sequencing of transposon insertion mutant libraries (Tn-Seq) represents a commonly used method to determine essential genes in bacteria. Using a hypersaturated transposon mutant library consisting of 400,096 unique Tn insertions, 523 genes ...
Donghui Choe +7 more
doaj +1 more source
Transposon variants and their effects on gene expression in arabidopsis [PDF]
, 2013 Transposable elements (TEs) make up the majority of many plant genomes. Their transcription and transposition is controlled through siRNAs and epigenetic marks including DNA methylation.
Smith, L.M., Wang, X., Weigel, D.
core +3 more sources
Construction and Screening of a Transposon Insertion Library of Yersinia enterocolitica (YeO3-R1)
Bio-Protocol, 2012 The Mu-transposon system is one of the best characterized transposition systems. Under minimal in vitro set-up, Mu transposition requires only a simple reaction buffer, MuA transposase protein, mini-Mu transposon DNA (donor) and target DNA.
Maria Pajunen +2 more
doaj +1 more source