Results 11 to 20 of about 121,903 (271)

Transposon-facilitated DNA sequencing. [PDF]

open access: yesProceedings of the National Academy of Sciences, 1991
We describe here a transposon-based DNA sequencing strategy that allows the introduction of sequencing priming sites throughout a target sequence by bacterial mating. A miniplasmid was designed to select against transposon insertions into the vector.
Strathmann, Michael   +5 more
openaire   +3 more sources

Self-synthesizing DNA transposons in eukaryotes [PDF]

open access: yesProceedings of the National Academy of Sciences, 2006
Eukaryotes contain numerous transposable or mobile elements capable of parasite-like proliferation in the host genome. All known transposable elements in eukaryotes belong to two types: retrotransposons and DNA transposons. Here we report a previously uncharacterized class of DNA transposons called Polintons that ...
Vladimir V, Kapitonov, Jerzy, Jurka
openaire   +2 more sources

DNA transposons in vertebrate functional genomics [PDF]

open access: yesCellular and Molecular Life Sciences, 2005
Genome sequences of many model organisms of developmental or agricultural importance are becoming available. The tremendous amount of sequence data is fuelling the next phases of challenging research: annotating all genes with functional information, and devising new ways for the experimental manipulation of vertebrate genomes.
C, Miskey   +3 more
openaire   +3 more sources

Drosophila parasitoid wasps bears a distinct DNA transposon profile. [PDF]

open access: yesMob DNA, 2018
The majority of Eukaryotic genomes are composed of a small portion of stable (non-mobile) genes and a large fraction of parasitic mobile elements such as transposable elements and endogenous viruses: the Mobilome. Such important component of many genomes are normally underscored in genomic analysis and detailed characterized mobilomes only exists for ...
da Silva AF   +3 more
europepmc   +5 more sources

The epigenetic regulation of HsMar1, a human DNA transposon. [PDF]

open access: yesBMC Genet, 2019
Both classes of transposable elements (DNA and RNA) are tightly regulated at the transcriptional level leading to the inactivation of transposition via epigenetic mechanisms. Due to the high copies number of these elements, the hypothesis has emerged that their regulation can coordinate a regulatory network of genes.
Renault S   +6 more
europepmc   +6 more sources

DNA methylation of fly genes and transposons [PDF]

open access: yesCellular and Molecular Life Sciences, 2006
The use of anti-5-methylcytosine antibodies in affinity columns allowed the identification of methylated sequences in the genome of Drosophila melanogaster adults. In view of the presence of transposable elements amongst the identified sequences, it has been suggested that DNA methylation is involved in transposon control in the fly genome.
MANDRIOLI, Mauro, BORSATTI, Federica
openaire   +2 more sources

Transposon facilitated DNA sequencing [PDF]

open access: yes, 1990
The purpose of this research is to investigate and develop methods that exploit the power of bacterial transposable elements for large scale DNA sequencing: Our premise is that the use of transposons to put primer binding sites randomly in target DNAs should provide access to all portions of large DNA fragments, without the inefficiencies of methods ...
Berg, D. E., Berg, C. M., Huang, H. V.
openaire   +2 more sources

Transposon display for active DNA transposons in rice

open access: yesGenes & Genetic Systems, 2007
Transposon display (TD) is a powerful technique to identify the integration site of transposons in gene tagging as a functional genomic tool for elucidating gene function. Although active endogenous DNA transposons have been used extensively for gene tagging in maize, only two active endogenous DNA transposons in rice have been identified, the 0.43-kb ...
Kyoko, Takagi   +4 more
openaire   +3 more sources

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