The CACTA transposon Bot1 played a major role in Brassica genome divergence and gene proliferation [PDF]
, 2008 We isolated and characterized a Brassica C genome-specific CACTA element, which was designated Bot1 (Brassica oleracea transposon 1). After analysing phylogenetic relationships, copy numbers and sequence similarity of Bot1 and Bot1 analogues in B ...
AGI, Arabidopsis Genome Initiative +80 more
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G3: Genes, Genomes, Genetics, 2016 DNA transposons and retroviruses are important transgenic tools for genome engineering. An important consideration affecting the choice of transgenic vector is their insertion site preferences.
Pavle Vrljicak +7 more
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The folding of the specific DNA recognition subdomain of the sleeping beauty transposase is temperature-dependent and is required for its binding to the transposon DNA. [PDF]
PLoS ONE, 2014 The reaction of DNA transposition begins when the transposase enzyme binds to the transposon DNA. Sleeping Beauty is a member of the mariner family of DNA transposons.
Gage O Leighton +5 more
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Transcriptome Analyses of Tumor-Adjacent Somatic Tissues Reveal Genes Co-Expressed with Transposable Elements [PDF]
, 2019 Background: Despite the long-held assumption that transposons are normally only expressed in the germ-line, recent evidence shows that transcripts of transposable element (TE) sequences are frequently found in the somatic cells.
Alberto, Adrian +9 more
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Domesticated DNA transposon proteins mediate retrotransposon control [PDF]
Cell Research, 2008 The Schizosaccharomyces pombe genome, like those of many eukaryotes, contains a number of retrotransposable repeat sequences. The pombe elements, termed Tf1 (transposon of fission yeast 1) and Tf2 possess long terminal repeats (LTRs) and belong to the gypsy family of retrotransposons [1]. This class of potentially deleterious mobile elements inserts at
Kathryn A, O'Donnell, Jef D, Boeke
openaire +2 more sources
Magic Pools: Parallel Assessment of Transposon Delivery Vectors in Bacteria
mSystems, 2018 Transposon mutagenesis coupled to next-generation sequencing (TnSeq) is a powerful approach for discovering the functions of bacterial genes. However, the development of a suitable TnSeq strategy for a given bacterium can be costly and time-consuming. To
Hualan Liu +8 more
doaj +3 more sources
BMC Biotechnology, 2019 Background DNA transposon-based vectors are effective nonviral tools for gene therapy and genetic engineering of cells. However, promoter DNA methylation and a near-random integration profile, which can result in transgene integration into ...
Kristian Alsbjerg Skipper +3 more
doaj +1 more source
DNA Transposons: Nature and Applications in Genomics [PDF]
Current Genomics, 2010 Repeated DNA makes up a large fraction of a typical mammalian genome, and some repetitive elements are able to move within the genome (transposons and retrotransposons). DNA transposons move from one genomic location to another by a cut-and-paste mechanism.
Muñoz-López, Martín +1 more
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Structural basis for DNA targeting by the Tn7 transposon [PDF]
Nature Structural & Molecular Biology, 2021 AbstractTn7 transposable elements are unique for their highly specific, and sometimes programmable, target-site selection mechanisms and precise insertions. All the elements in the Tn7-family utilize a AAA+ adaptor (TnsC) to coordinates target-site selection with transposase activation and prevent insertions at sites already containing a Tn7 element ...
Yao Shen +5 more
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Beta-Lactamase Repressor BlaI Modulates Staphylococcus aureus Cathelicidin Antimicrobial Peptide Resistance and Virulence. [PDF]
, 2015 BlaI is a repressor of BlaZ, the beta-lactamase responsible for penicillin resistance in Staphylococcus aureus. Through screening a transposon library in S.
Gallo, Richard L +6 more
core +6 more sources