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Tn5 DNA Transposase in Multi-Omics Research
Methods and Protocols, 2023 Tn5 transposase use in biotechnology has substantially advanced the sequencing applications of genome-wide analysis of cells. This is mainly due to the ability of Tn5 transposase to efficiently transpose DNA essentially randomly into any target DNA ...
Dmitry Penkov +2 more
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Frontiers in Plant Science, 2022 Mariner-like elements (MLEs) are promising tools for gene cloning, gene expression, and gene tagging. We have characterized two MLE transposons from moso bamboo, Ppmar1 and Ppmar2. Ppmar2, is smaller in size and has higher natural activities, thus making
Xiaohong Zhou +5 more
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The first discovery of Tc1 transposons in yeast
Frontiers in Microbiology, 2023 BackgroundIdentification of transposons without close homologs is still a difficult task. IS630/Tc1/mariner transposons, classified into a superfamily, are probably the most widespread DNA transposons in nature.
Jia Chang +10 more
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Emerging Infectious Diseases, 2023 Incidence of Streptococcus dysgalactiae subspecies equisimilis (SDSE) bacteremia is increasing in the Kyoto-Shiga region of Japan. We retrospectively analyzed clinical features of SDSE bacteremia and conducted comparative genomic analyses of isolates ...
Koh Shinohara +8 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2022 In recent years, mammalian cells have become the primary host cells for the production of recombinant therapeutic proteins (RTPs). Despite that the expression of RTPs in mammalian cells can be improved by directly optimizing or engineering the expression
Mian Wei +4 more
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Insertion Specificity of the hATx-6 Transposase of Hydra magnipapillata
Frontiers in Molecular Biosciences, 2021 Transposable elements (TE) are mobile genetic elements, present in all domains of life. They commonly encode a single transposase enzyme, that performs the excision and reintegration reactions, and these enzymes have been used in mutagenesis and creation
Paul Riggs +5 more
doaj +1 more source
Biochemical analysis of nucleosome targeting by Tn5 transposase [PDF]
Open Biology, 2019 Tn5 transposase is a bacterial enzyme that integrates a DNA fragment into genomic DNA, and is used as a tool for detecting nucleosome-free regions of genomic DNA in eukaryotes.
Shoko Sato +7 more
doaj +1 more source
Functional characterization of the Bari1 transposition system. [PDF]
PLoS ONE, 2013 The transposons of the Bari family are mobile genetic elements widespread in the Drosophila genus. However, despite a broad diffusion, virtually no information is available on the mechanisms underlying their mobility.
Antonio Palazzo +6 more
doaj +1 more source
Transcription activator like effector (TALE)-directed piggyBac transposition in human cells. [PDF]
, 2013 Insertional therapies have shown great potential for combating genetic disease and safer methods would undoubtedly broaden the variety of possible illness that can be treated. A major challenge that remains is reducing the risk of insertional mutagenesis
Bhakta, Mital S +6 more
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Tn6188 - A Novel Transposon in Listeria monocytogenes Responsible for Tolerance to Benzalkonium Chloride [PDF]
, 2013 peer-reviewedControlling the food-borne pathogen Listeria (L.) monocytogenes is of great importance from a food safety perspective, and thus for human health.
Cotter, Paul D. +8 more
core +9 more sources