Identification and characterization of domesticated bacterial transposases [PDF]
Genome Biology and Evolution, 2017 ABSTRACTSelfish genetic elements (SGEs), such as insertion sequences (ISs) and transposons are found in most genomes. Transposons are usually identifiable by their high copy number within genomes. In contrast REP associated tyrosine transposases (RAYTs), a recently described class of bacterial transposase, are typically present at just one ...
Bertels, Frederic +2 more
openaire +3 more sources
RAG-1 Mutations Associated with B-Cell-Negative SCID Dissociate the Nicking and Transesterification Steps of V(D)J Recombination [PDF]
, 2001 Some patients with B-cell-negative severe combined immune deficiency (SCID) carry mutations in RAG-1 or RAG-2 that impair V(D)J recombination. Two recessive RAG-1 mutations responsible for B-cell-negative SCID, R621H and E719K, impair V(D)J recombination
Chang, Fu-Chung +2 more
core +2 more sources
Structural Determinants of Sleeping Beauty Transposase Activity [PDF]
Molecular Therapy, 2016 Transposases are important tools in genome engineering, and there is considerable interest in engineering more efficient ones. Here, we seek to understand the factors determining their activity using the Sleeping Beauty transposase. Recent work suggests that protein coevolutionary information can be used to classify groups of physically connected ...
Abrusán, György +7 more
openaire +4 more sources
A Hyperactive Transposase Promotes Persistent Gene Transfer of a piggyBac DNA Transposon
Molecular Therapy: Nucleic Acids, 2012 Nonviral vector systems are used increasingly in gene targeting and gene transfer applications. The piggyBac transposon represents an alternative integrating vector for in vivo gene transfer. We hypothesized that this system could achieve persistent gene
Erin R Burnight +7 more
doaj +1 more source
Isolation and characterization of Tn7 transposase gain-of-function mutants: a model for transposase activation [PDF]
The EMBO Journal, 2000 Tn7 transposition has been hypothesized to require a heteromeric transposase formed by two Tn7-encoded proteins, TnsA and TnsB, and accessory proteins that activate the transposase when they are associated with an appropriate target DNA. This study investigates the mechanism of Tn7 transposase activation by isolation and analysis of transposase gain-of-
F, Lu, N L, Craig
openaire +2 more sources
The Role of Vertical and Horizontal Transfer in the Evolutionary Dynamics of PIF-Like Transposable Elements in Triticeae. [PDF]
PLoS ONE, 2015 PIF-like transposable elements are members of the PIF/Harbinger superfamily of DNA transposons found in the genomes of many plants, animals, and fungi.
Dragomira N Markova +1 more
doaj +1 more source
Pattern Formation in Semiconductors [PDF]
, 1999 In semiconductors, nonlinear generation and recombination processes of free carriers and nonlinear charge transport can give rise to non-equilibrium phase transitions.
BK Ridley +11 more
core +1 more source
Simplified Epigenome Profiling Using Antibody-tethered Tagmentation
Bio-Protocol, 2021 We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples and ...
Steven Henikoff +2 more
doaj +1 more source
Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development [PDF]
, 2014 Peer reviewedPublisher ...
Adams JC +67 more
core +1 more source
Evidence of horizontal gene transfer by transposase gene analyses in Fervidobacterium species. [PDF]
PLoS ONE, 2017 Horizontal Gene Transfer (HGT) plays an important role in the physiology and evolution of microorganisms above all thermophilic prokaryotes. Some members of the Phylum Thermotogae (i.e., Thermotoga spp.) have been reported to present genomes constituted ...
Alba Cuecas +2 more
doaj +1 more source