Results 31 to 40 of about 68,477 (364)

Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering [PDF]

Nature Communications, 2016
Sleeping Beauty (SB) is a prominent Tc1/mariner superfamily DNA transposon that provides a popular genome engineering tool in a broad range of organisms.
Ivics, Z., Sebe, A., Izsvák, Zsuzsanna, Ivics, Zoltán, Voigt, Franka, Sebe, Attila, Mátés, Lajos, Barabas, Orsolya, Wiedemann, Lisa, Voigt, F., Querques, I., Mátés, L., Querques, Irma, Izsvák, Z., Wiedemann, L., Zuliani, C., Zuliani, Cecilia, Barabas, O.   +17 more
core   +2 more sources

Structural determinants of Sleeping Beauty transposase activity [PDF]

Molecular Therapy, 2016
Transposases are important tools in genome engineering, and there is considerable interest in engineering more efficient ones. Here, we seek to understand the factors determining their activity using the Sleeping Beauty transposase. Recent work suggests
Ivics, Z., Marsh, Joseph A., Abrusán, G., Izsvák, Zsuzsanna, Ivics, Zoltán, Yant, S. R., Abrusan, Gyorgy, Szilágyi, András, Mátés, Lajos, Barabás, O., Yant, Stephen, Marsh, Joseph; id_orcid, Yant, S.R., Mátés, L., Barabás, Orsolya, Szilágyi, A., Marsh, J.A., Izsvák, Z., Yant, Stephen R., Marsh, J. A.   +19 more
core   +5 more sources

Mutator Transposase Is Widespread in the Grasses [PDF]

Plant Physiology, 2001
Abstract Although the Mutator (Mu) system is well characterized in maize (Zea mays), very little is known about this highly mutagenic system of transposons in other grasses. Mutator is regulated by the MuDRclass of elements, which encodes two genes, one of which,mudrA, has similarity to a number of bacterial transposases.
Damon Lisch, Michael Freeling, Richard J. Langham, Ming Y. Choy   +3 more
openalex   +4 more sources

First Mariner Mos1 Transposase Inhibitors

Mini reviews in medicinal chemistry, 2009
International audienceWe described chemical inhibitors of Mos1 transposition. Some were already known to affect a related prokaryotic transposase (Tn5) or HIV-1 integrase, whereas the other were new compounds in this field.
Viaud-Massuard, Maire-Claude, Bouchet, Nicolas, Bigot, Yves, Auge-Gouillou, Corinne, Delelis, Olivier, Dubernet, Mathieu, Guillard, Jerome, Ryabinin, Vladimir, Bischerour, Julien, Germon, Stephanie   +9 more
core   +3 more sources

Metaproteomics Reveals Abundant Transposase Expression in Mutualistic Endosymbionts

mBio, 2013
Transposases, enzymes that catalyze the movement of mobile genetic elements, are the most abundant genes in nature. While many bacteria encode an abundance of transposases in their genomes, the current paradigm is that the expression of transposase genes
Manuel Kleiner, Jacque C. Young, Manesh Shah, Nathan C. VerBerkmoes, Nicole Dubilier   +4 more
doaj   +4 more sources

Structure of the TnsB transposase-DNA complex of type V-K CRISPR-associated transposon

bioRxiv, 2022
The cryo-EM structure of the type VK CRISPR-associated TnsB transposase sheds light onto RNA-guided transposition, providing new possibilities to redesign CRISPR-associated transposon systems.
Francisco Tenjo-Castaño, N. Sofos, B. López-Méndez, Luisa S. Stutzke, A. Fuglsang, S. Stella, G. Montoya   +6 more
semanticscholar   +1 more source

Artificial optimization of bamboo Ppmar2 transposase and host factors effects on Ppmar2 transposition in yeast

Frontiers in Plant Science, 2022
Mariner-like elements (MLEs) are promising tools for gene cloning, gene expression, and gene tagging. We have characterized two MLE transposons from moso bamboo, Ppmar1 and Ppmar2. Ppmar2, is smaller in size and has higher natural activities, thus making
Xiaohong Zhou, Jiamin Xie, Chao Xu, Xiuling Cao, Long-Hai Zou, Mingbing Zhou   +5 more
doaj   +1 more source

Investigating the Trans Effects of IS<italic>1</italic> Transposases on Intragenomic DNA Movements in <italic>Escherichia coli</italic>. [PDF]

Microb Physiol
Ord JW, Zhang Z, Wu J, Smith S, Feng Z, Ancheta K, Garrido LF, Yao G, Gupta S, Lee ET, Saier MH.   +10 more
europepmc   +3 more sources

Tn5 transposase-based epigenomic profiling methods are prone to open chromatin bias

bioRxiv, 2021
Epigenetic studies of rare biological samples like mammalian oocytes and preimplantation embryos require low input or even single cell epigenomic profiling methods. To reduce sample loss and avoid inefficient immunoprecipitation, several chromatin immuno-
Meng Wang, Yi Zhang
semanticscholar   +1 more source

The first discovery of Tc1 transposons in yeast

Frontiers in Microbiology, 2023
BackgroundIdentification of transposons without close homologs is still a difficult task. IS630/Tc1/mariner transposons, classified into a superfamily, are probably the most widespread DNA transposons in nature.
Jia Chang, Guangyou Duan, Wenjing Li, Wenjing Li, Tung On Yau, Chang Liu, Jianlin Cui, Huaijun Xue, Wenjun Bu, Yanping Hu, Shan Gao   +10 more
doaj   +1 more source