Results 111 to 120 of about 68,477 (364)

High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from ‘Candidatus Liberibacter asiaticus’

Journal of Integrative Agriculture, 2015
Two miniature inverted-repeat transposable elements (MITEs), MCLas-A and MCLas-B, were recently identified from ‘Candidatus Liberibacter asiaticus’ known to be associated with citrus Huanglongbing (HLB, yellow shoot disease).
Xue-feng WANG, Jiao-yue CHEN, Jin TAN, Suo DUAN, Xiao-ling DENG, Jian-chi CHEN, Chang-yong ZHOU   +6 more
doaj   +1 more source

A Transposon and Transposase System for Human Application

Molecular Therapy, 2010
The stable introduction of therapeutic transgenes into human cells can be accomplished using viral and nonviral approaches. Transduction with clinical-grade recombinant viruses offers the potential of efficient gene transfer into primary cells and has a record of therapeutic successes.
Hackett, Perry B, Largaespada, David A, Cooper, Laurence JN   +2 more
openaire   +2 more sources

3D Chromatin Architecture Provides Insights Into Leaf Trait Variation Among Pear Species

Advanced Science, EarlyView.
ABSTRACT Three‐dimensional (3D) chromatin architecture plays a fundamental role in eukaryotic gene regulations, its functional significance in perennial fruit trees remains poorly characterized despite extensive applications in crop genomics. Here, we developed high‐resolution (∼5 kb) Hi‐C maps of Pyrus and compared 3D genomic architecture of three ...
Yueyuan Liu, Chenhui Han, Cheng Xue, Manyi Sun, Bobo Song, Yongsong Xue, Guangyan Yang, Shaozhuo Xu, Wei Wei, Jiaming Li, Runze Wang, Jun Wu   +11 more
wiley   +1 more source

IS21 family transposase cleaved donor complex traps two right-handed superhelical crossings

Nature Communications, 2023
Transposases are ubiquitous enzymes that catalyze DNA rearrangement events with broad impacts on gene expression, genome evolution, and the spread of drug-resistance in bacteria.
Mercedes Spínola-Amilibia, Lidia Araújo-Bazán, Álvaro de la Gándara, James M. Berger, Ernesto Arias-Palomo   +4 more
doaj   +1 more source

T Cell Exhaustion in Cancer Immunotherapy: Heterogeneity, Mechanisms, and Therapeutic Opportunities

Advanced Science, EarlyView.
T cell exhaustion limits immunotherapy efficacy. This article delineates its progression from stem‐like to terminally exhausted states, governed by persistent antigen, transcription factors, epigenetics, and metabolism. It maps the exhaustion landscape in the TME and proposes integrated reversal strategies, providing a translational roadmap to overcome
Yang Yu, Xiaoyu Yao, Qingyang Wang, Mengrui Yang, Runze Li, Jiangjiang Qin, Jing Zhuang, Changgang Sun   +7 more
wiley   +1 more source

DNA binding activities of the Herves transposase from the mosquito Anopheles gambiae

Mobile DNA, 2011
Background Determining the mechanisms by which transposable elements move within a genome increases our understanding of how they can shape genome evolution.
Kahlon Amandeep S, Hice Robert H, O'Brochta David A, Atkinson Peter W   +3 more
doaj   +1 more source

Amino-Terminal Sequence of the Tn3 Transposase Protein [PDF]

, 1982
The amino-terminal sequence of the Tn transposase protein was determined to be Pro-Val-Asp-Phe-Leu-Thr-Thr-Glu-Gln-Val-Glu-Ser.... This was determined both from an active transposase protein purified from a transposase overproducing mutant strain and ...
Fennewald, Michael A., Cohen, Stanley N., Chou, Joany, Hood, Leroy E., Ditto, Mary D., Gerrard, Stephen P., Hunkapiller, Michael W., Casadaban, Malcolm J,   +7 more
core  

Transposase-mediated integration events of ‘solo’ substrates.

, 2014
HeLa cells were co-transfected with the ‘solo’ transposon constructs in the present of either mPB or SB100X transposases, while a catalytically inactive SB transposase, D3 was used as a control.
Anatharam Devaraj (535104), Jichang Wang (466681), Zsuzsanna Izsvák (207897), Zoltán Ivics (196129), Matthias Selbach (317683), Ana Jimenez Orgaz (535105), Manvendra Singh (116654), Yongming Wang (406450), Jia-Xuan Chen (535106)   +8 more
core   +1 more source

Transactivation of Ds by Ac-transposase gene fusions in tobacco [PDF]

, 1992
To study regulation of the (Ds) transposition process in heterologous plant species, the transposase gene of Ac was fused to several promoters that are active late during plant development.
Rommens, Caius M.T.,, Hille, Jacques,, Tunen, Arjen J. van, Mol, Joseph N.M.,, Rommens, Caius M.T., Nijkamp, H. John J.,, Hille, Jacques, Buchel, Annemarie S., Haaren, Mark J.J. van, Tunen, Arjen J. van,, Nijkamp, H. John J., Mol, Joseph N.M., Haaren, Mark J.J. van,, Buchel, Annemarie S.,   +13 more
core   +1 more source

Cis‐ and Trans‐Regulatory Factors Independently Shape Phenotypic Heterogeneity of Retinitis Pigmentosa

Advanced Science, EarlyView.
A zebrafish model carrying an identical human RHO S334X allele reveals two independent genetic layers shaping retinitis pigmentosa (RP) severity: a protective 3‐bp cis‐regulatory insertion that attenuates transgene expression, and a dominant trans‐acting modifier that restores a severe phenotype.
Cong Cui, Kotone Nakagawa, Takumi Tateno, Ayaka Dan, Dexin Meng, Yoshihiro Omori, Soma Tomihara, Suzuri Okamoto, Shigeru Sato, Motokazu Tsujikawa   +9 more
wiley   +1 more source