Results 131 to 140 of about 17,057 (320)

Engineering Microbial Particles for Next‐Generation Biomedical Platforms

Advanced Science, EarlyView.
Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li, Yunjie Lu, Ziyan Huang, Jianjiang Chen, Yanbin Liu, Lei Qin, Chao Wang, Yumin Wu   +7 more
wiley   +1 more source

CRISPR in mobile genetic elements: counter-defense, inter-element competition and RNA-guided transposition

BMC Biology
CRISPR are adaptive immunity systems that protect bacteria and archaea from viruses and other mobile genetic elements (MGE) via an RNA-guided interference mechanism.
Eugene V. Koonin, Kira S. Makarova
doaj   +1 more source

Transposition of Insertion Sequences was Triggered by Oxidative Stress in Radiation-Resistant Bacterium Deinococcus geothermalis

Microorganisms, 2019
During an oxidative stress-response assay on a putative Dps-like gene-disrupted Δdgeo_0257 mutant strain of radiation-resistant bacterium Deinococcus geothermalis, a non-pigmented colony was observed among the normal reddish color colonies. This non-
Chanjae Lee, Nakjun Choi, Min K. Bae, Kyungsil Choo, Sung-Jae Lee   +4 more
doaj   +1 more source

ACLY‐Driven Metabolic Reprogramming Promotes Histone Acetylation and Inflammation‐Associated Fibrosis in Chronic Kidney Disease

Advanced Science, EarlyView.
This study reveals a citrate–ACLY–H3K27ac metabolic–epigenetic axis driving inflammatory gene activation and kidney fibrosis, highlighting ACLY inhibition as a potential therapeutic strategy for chronic kidney disease (CKD). ABSTRACT The mechanisms by which metabolic stress drives epigenetic dysregulation and fibrosis in chronic kidney disease (CKD ...
Chunxiu Du, Dhanunjay Mukhi, Lingzhi Li, Chenyu Li, Siyu Pan, Bernhard Dumoulin, Eunji Ha, Lakshmi P Kolligundla, Yanjuan Hou, Jonathan Levinsohn, Chaelin Kang, Konstantin Adrian Klötzer, Junnan Wu, Samer Mohandes, Kathryn E Wellen, Katalin Susztak   +15 more
wiley   +1 more source

A bend, flip and trap mechanism for transposon integration

eLife, 2016
Cut-and-paste DNA transposons of the mariner/Tc1 family are useful tools for genome engineering and are inserted specifically at TA target sites. A crystal structure of the mariner transposase Mos1 (derived from Drosophila mauritiana), in complex with ...
Elizabeth R Morris, Heather Grey, Grant McKenzie, Anita C Jones, Julia M Richardson   +4 more
doaj   +1 more source

A Viscous DES‐AAV‐Foxo1 Delivery System With High Transfection Efficiency for the Treatment of Corneal Endothelial Dysfunction by Restoring Mitochondria‐ER Contacts

Advanced Science, EarlyView.
High glucose triggers corneal endothelial dysfunction by impairing FOXO1‐mediated ITPR1 transcription, leading to disrupted mitochondria‐associated membrane (MAM) integrity and defective ER‐to‐mitochondria Ca2+ transfer. This study develops an innovative viscous DES‐AAV‐Foxo1 delivery system with enhanced transfection efficiency. This non‐invasive gene
Hongran Zhao, Xiaoyu Li, Hongwei Wang, Zongyi Li, Qun Wang, Yangyang Zhang, Xu Jing, Xia Qi, Qingjun Zhou, Shengqian Dou, Lixin Xie   +10 more
wiley   +1 more source

DNA binding activities of the Herves transposase from the mosquito Anopheles gambiae

Mobile DNA, 2011
Background Determining the mechanisms by which transposable elements move within a genome increases our understanding of how they can shape genome evolution.
Kahlon Amandeep S, Hice Robert H, O'Brochta David A, Atkinson Peter W   +3 more
doaj   +1 more source

Genetic Code Expanded T Cell for Controllable Immunotherapy

Advanced Science, EarlyView.
Our GCE‐CAR‐T cells enables tight, dose‐dependent, and function‐preserving control of CAR expression at the translational level through amber codon suppression and genetic incorporation of ncAA. ABSTRACT Chimeric antigen receptor (CAR)‐T cell therapy has demonstrated curative potential against hematologic malignancies, but its clinical application ...
Xue Wang, Yingao Gao, Yeyu Su, Yong Wang, Tao Liu   +4 more
wiley   +1 more source

Eco‐Geography Reverses Dominant AMR Reservoirs in Klebsiella pneumoniae: Integron‐Rich Mobilomes and Cross‐Niche Connectivity

Advanced Science, EarlyView.
Dominant antimicrobial resistance reservoirs in Klebsiella pneumoniae vary across eco‐geographic settings rather than following a universal pattern. Integrated One Health and global genomic analyses show that lineage structure, integron load, and cross‐niche connectivity shape whether AMR burden accumulates primarily in human or nonhuman compartments ...
Hui Lin, Biao Tang, Hao Xu, Jiajie Qian, Feifan Shi, Junhui Zhao, Xiaorui Mao, Xiaohe Hu, Ruishan Liu, Wenhong Liu, Xiawei Jiang, Beiwen Zheng, Guoping Zhao   +12 more
wiley   +1 more source

Genome data cross-contamination versus interdomain recombination: Equus caballus and Mus musculus genetic loci in the insertion sequence-rich genomes of two clonally related methicillin-resistant Staphylococcus aureus strains from China

BMC Microbiology
Background Methicillin-resistant Staphylococcus aureus (MRSA) represents a significant global health threat, responsible for infections in both humans and animals. Determining genetic patterns associated with the genome plasticity of MRSA is critical for
Rusudan Tsiklauri, Saba Kobakhidze, Megi Tsereteli, Lilia Jimsherishvili, Nata Kakabadze, Stylianos Koulouris, Mamuka Kotetishvili   +6 more
doaj   +1 more source