Results 111 to 120 of about 16,112 (276)

Transposase injection mix protocol v1 [PDF]

, 2019
This protocol details the steps necessary to generate injection mixes (comprised of plasmid DNA for integration and transposase mRNA) for transposon-mediated integration into Aedes aegypti and other insects. It has been empirically tested in Ae. aegypti embryos with piggyBac transposase.
openaire   +1 more source

Cancer‐Associated Fibroblasts Promote Tumor Immunosuppression in Hepatocellular Carcinoma via the NNMT‐ANGPTL4 Axis

Advanced Science, EarlyView.
ABSTRACT Background & Aims: Cancer‐associated fibroblasts (CAFs) drive immunosuppression in hepatocellular carcinoma (HCC). However, their metabolic regulation remains poorly defined. We investigated the role of nicotinamide N‐methyltransferase (NNMT) in CAFs. Approach & Results: High NNMT expression in CAF tissues was confirmed by western blotting and
Shounan Lu, Shanjia Ke, Hongjun Yu, Zhanzhi Meng, Miaoyu Bai, Yanan Xu, Hui Zhu, Jinwen Yang, Baolin Qian, Bing Yin, Chaoqun Wang, Zhigang Feng, Zhongyu Li, Yongzhi Zhou, Zihao Li, Xinglong Li, Yongliang Hua, Yao Fu, Wei Tang, Yaohua Wu, Yong Ma   +20 more
wiley   +1 more source

Germline transgenesis and insertional mutagenesis in the ascidian Ciona intestinalis [PDF]

, 2007
Stable transgenesis is a splendid technique that is applicable to the creation of useful marker lines,enhancer/gene traps, and insertional mutagenesis.
Sasakura Yasunori, 笹倉 靖徳
core   +1 more source

Dose‐Dependent Reprogramming of Chromatin Accessibility by SOX4 Drives the Transcriptional Response to Iron Overload

Advanced Science, EarlyView.
This study demonstrates that iron overload triggers widespread chromatin compaction and transcriptional repression in human granulosa cells, recapitulating features of endometriosis. The epigenetic reprogramming is orchestrated by a TFEB‐SOX4‐SWI/SNF axis, with SOX4 acting as a central, dosage‐sensitive regulator.
Feifei Li, Yaoqiu Wu, Guangyu Yang, Jingyi Lai, Xiaoyue Sun, Liyan Wang, Xiaoli Li, Jing Zhang, Qingxue Zhang, Hui Chen, Haiyan Lin, Bingxiang Xu, Junfeng Zhang, Hailong Wang, Anming Meng, Chunwei Cao   +15 more
wiley   +1 more source

Lactobacillus delbrueckii ssp. lactis and ssp. bulgaricus: a chronicle of evolution in action [PDF]

, 2014
Lactobacillus delbrueckii ssp. lactis and ssp. bulgaricus are lactic acid producing bacteria that are largely used in dairy industries, notably in cheese-making and yogurt production.
Benoît Quinquis, Bouziane Moumen, Emmanuelle Maguin, Hela El Kafsi, Jean-Michel Batto, Johan Binesse, Julien Buratti, Maarten van de Guchte, Nathalie Galleron, Rozenn Dervyn, Samira Boudebbouze, Sean Kennedy, Valentin Loux   +12 more
core   +4 more sources

Leveraging Artificial Intelligence and Large Language Models for Cancer Immunotherapy

Advanced Science, EarlyView.
Cancer immunotherapy faces challenges in predicting treatment responses and understanding resistance mechanisms. Artificial intelligence (AI) and machine learning (ML) offer powerful solutions for cancer immunotherapy in patient stratification, biomarker discovery, treatment strategy optimization, and foundation model development.
Xinchao Wu, Mengtao Sun, Lusheng Li, Jieqiong Wang, Shibiao Wan   +4 more
wiley   +1 more source

Global transcriptomic analyses of Salmonella enterica in Iron-depleted and Iron-rich growth conditions

BMC Genomics, 2019
Background Salmonella enterica possess several iron acquisition systems, encoded on the chromosome and plasmids. Recently, we demonstrated that incompatibility group (Inc) FIB plasmid-encoded iron acquisition systems (Sit and aerobactin) likely play an ...
Bijay K. Khajanchi, Joshua Xu, Christopher J. Grim, Andrea R. Ottesen, Padmini Ramachandran, Steven L. Foley   +5 more
doaj   +1 more source

Tame affairs: domesticated transposase and domestic pigs [PDF]

EMBO reports, 2010
Long thought to be genomic junk, domesticated transposons have turned out to be an incredible source of new genes. Two studies—one this issue of EMBO reports—show that a domesticated hAT transposase is a transcriptional repressor of IGF2 that regulates muscle growth and has over 1,200 additional putative targets in the mammalian genome.
openaire   +2 more sources

A Circuit of Mechanically Regulated Transcription Factors Balances Regenerative and Fibrotic Memory of Mesenchymal Stromal Cells

Advanced Science, EarlyView.
Producing MSCs on rigid culture substrates induces a scar‐making phenotype, jeapordizing therapeutic success. ‘Tissue‐soft’ surfaces prevent MSC fibrogenesis and preserve regenerative traits. An epigenetic network, driven by HOXA11 and SALL1, maintains ‘soft memory’ by keeping chromatin open in relaxed MSCs, promoting anti‐fibrotic programs.
Fereshteh Sadat Younesi, Andrew E. Miller, Li Diao, Xinying Guo, Natalie Andonian, Elham Karimizadeh, Thomas H. Barker, Boris Hinz   +7 more
wiley   +1 more source

Transposition of Insertion Sequences was Triggered by Oxidative Stress in Radiation-Resistant Bacterium Deinococcus geothermalis

Microorganisms, 2019
During an oxidative stress-response assay on a putative Dps-like gene-disrupted Δdgeo_0257 mutant strain of radiation-resistant bacterium Deinococcus geothermalis, a non-pigmented colony was observed among the normal reddish color colonies. This non-
Chanjae Lee, Nakjun Choi, Min K. Bae, Kyungsil Choo, Sung-Jae Lee   +4 more
doaj   +1 more source