Results 41 to 50 of about 111,214 (295)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Monitoring of hepatitis E virus infection and replication by functional tagging of the ORF2 protein

JHEP Reports
Background and Aims: Hepatitis E virus (HEV) infection is a leading cause of acute hepatitis worldwide. Understanding of the mechanisms underlying productive HEV infection remains incomplete and would benefit from technological advances improving current
Maliki Ankavay, Nathalie Da Silva, Angela Pollán, Noémie Oechslin, Katja Dinkelborg, Patrick Behrendt, Darius Moradpour, Jérôme Gouttenoire   +7 more
doaj   +1 more source

Advanced Materials for Biologics Delivery to Brain Tumors

Advanced Materials, EarlyView.
Material innovation is central to unlocking the therapeutic potential of biologics against many central nervous system diseases, including brain cancer. By engineering carriers with controlled transport, targeting, and release properties, advanced materials can overcome the blood–brain barrier and tumor microenvironment, improving the delivery of ...
Yuran Feng, Yuxue Cao, Maria Kavallaris, Taskeen Janjua, Amirali Popat   +4 more
wiley   +1 more source

Embryonic piRNAs target horizontally transferred vertebrate transposons in assassin bugs

Frontiers in Cell and Developmental Biology
IntroductionPiwi proteins and the associated Piwi-interacting RNAs (piRNAs) coordinate a surveillance system that protects the animal genome from DNA damage induced by transposable element (TE) mobilization. While the pathway has been described in detail
Tarcísio Fontenele de Brito, Maira Arruda Cardoso, Nazerke Atinbayeva, Nazerke Atinbayeva, Ingrid Alexandre de Abreu Brito, Lucas Amaro da Costa, Nicola Iovino, Attilio Pane, Attilio Pane   +8 more
doaj   +1 more source

Improved transposon-based library preparation for the Ion Torrent platform

BioTechniques, 2015
A transposon-based approach for the construction of sequencing libraries is an efficient way of preparing samples for processing on both Illumina and Ion Torrent platforms.
Tatyana Gorbacheva, Wilber Quispe-Tintaya, Vasily N. Popov, Jan Vijg, Alexander Y. Maslov   +4 more
doaj   +1 more source

A hybrid lentivirus-transposon vector for safer gene therapy [PDF]

, 2010
Gene therapy vectors based on the HIV-1 lentivirus are an attractive option for clinical applications because they enter a broad range of target cells efficiently and deliver stable gene expression through integration into host chromosomes.
Vink, C.A.
core  

Transposon insertion mutants.

, 2022
(A) Schematic depicting HESA-selected transposon insertion within lipopolysaccharide clusters LPSI, LPSII, the quorum sensing cluster and the YeRER. Vertical arrows denote transposon insertion points.
Campbell R. Sheen (10325439), Mark R. H. Hurst (11999109), Travis R. Glare (6887828), Maureen O’Callaghan (3440684), Marion Schoof (11999106)   +4 more
core   +1 more source

Engineering Approaches to Modify Immunomodulatory Functions of Mesenchymal Stromal Cells (MSCs): Tissue Regeneration and Clinical Application

Advanced Science, EarlyView.
Mesenchymal stromal cells (MSCs) show promise for treating immune‐related disorders through immunomodulation and tissue regeneration. This review gives a brief overview of current clinical approval of MSC therapies. It also discussed how bioengineering, including genetic modification, biomaterial delivery, extracellular vesicles, and iPSC‐derived MSCs,
Sichen Yang, Kejia Li, Ning Wang, Zhen Li, Zhiyong Zhang, Rocky S. Tuan, Yangzi Jiang   +6 more
wiley   +1 more source

First Report in the Americas of S. enterica Var. Enteritidis Carrying bla_NDM-1 in a Putatively New Sub-Lineage of IncC2 Plasmids

Antibiotics
Background: Infections caused by carbapenem-resistant Enterobacterales have steadily multiplied over time, becoming a major threat to healthcare systems due to limited therapeutic options and high case-fatality rates.
Nicolás F. Cordeiro, Romina Papa-Ezdra, Germán Traglia, Inés Bado, Virginia García-Fulgueiras, María N. Cortinas, Leticia Caiata, Mariana López-Vega, Ana Otero, Martín López, Patricia Hitateguy, Cristina Mogdasy, Rafael Vignoli   +12 more
doaj   +1 more source

RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops

Advanced Science, EarlyView.
This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Xiong Zhang, Yupo Wu, Jiachang Hou, Lixia Gao, Longqi Pan, Cuicui Shen, Chuanji Zhao, Feng Gao, Yi Zhang, Li Ren, Xiaoping Fang, Xiaoling Dun, Shengyi Liu, Lijiang Liu   +13 more
wiley   +1 more source