Transposon mutagenesis in a hyper-invasive clinical isolate of Campylobacter jejuni reveals a number of genes with potential roles in invasion [PDF]
, 2010 Transposon mutagenesis has been applied to a hyper-invasive clinical isolate of Campylobacter jejuni, 01/51. A random transposon mutant library was screened in an in vitro assay of invasion and 26 mutants with a significant reduction in invasion were ...
Bagnall, MC +5 more
core +1 more source
Transcription activator like effector (TALE)-directed piggyBac transposition in human cells. [PDF]
, 2013 Insertional therapies have shown great potential for combating genetic disease and safer methods would undoubtedly broaden the variety of possible illness that can be treated. A major challenge that remains is reducing the risk of insertional mutagenesis
Bhakta, Mital S +6 more
core +3 more sources
Global Spread of Multiple Aminoglycoside Resistance Genes
Emerging Infectious Diseases, 2005 Emergence of the newly identified 16S rRNA methylases RmtA, RmtB, and ArmA in pathogenic gram-negative bacilli has been a growing concern. ArmA, which had been identified exclusively in Europe, was also found in several gram-negative pathogenic bacilli ...
Kunikazu Yamane +4 more
doaj +1 more source
Histone Demethylase AMX-1 Regulates Fertility in a p53/CEP-1 Dependent Manner
Frontiers in Genetics, 2022 Histone methylation shapes the epigenetic configuration and adjusts multiple fundamental nuclear processes, including transcription, cell cycle control and DNA repair.
Xiaojing Ren +4 more
doaj +1 more source
Inactivation of the major hemolysin gene influences expression of the nonribosomal peptide synthetase gene swrA in the insect pathogen Serratia sp. strain SCBI [PDF]
, 2017 Hemolysins are important virulence factors for many bacterial pathogens, including Serratia marcescens. The role of the major hemolysin gene in the insect pathogen Serratia sp.
Bode, Helge +4 more
core +2 more sources
Frontiers in Microbiology, 2023 Microalgae are a large group of organisms that can produce various useful substances through photosynthesis. Microalgae need to be genetically modified at the molecular level to become “Chassis Cells” for food, medicine, energy, and environmental ...
Xiaobing Hu +6 more
doaj +1 more source
In vitro and in vivo screening for novel essential cell-envelope proteins in Pseudomonas aeruginosa [PDF]
, 2015 The Gram-negative bacterium Pseudomonas aeruginosa represents a prototype of multi-drug resistant opportunistic pathogens for which novel therapeutic options are urgently required.
Bragonzi, Alessandra +5 more
core +1 more source
In vitro amplification of whole large plasmids via transposon-mediated oriC insertion
BioTechniques, 2021 DNA amplification is a fundamental technique in molecular biology. The replication cycle reaction is a new method for amplification of large circular DNA having oriC sequences, which is a replication initiation site of the Escherichia coli chromosome. We
Seia Nara, Masayuki Su'etsugu
doaj +1 more source
Chimeric piggyBac transposases for genomic targeting in human cells. [PDF]
, 2012 Integrating vectors such as viruses and transposons insert transgenes semi-randomly and can potentially disrupt or deregulate genes. For these techniques to be of therapeutic value, a method for controlling the precise location of insertion is required ...
Belcaid, Mahdi +9 more
core +5 more sources
Microbiology Spectrum, 2022 Mobile genetic elements (MGEs) are often associated with antimicrobial resistance genes (ARGs). They are responsible for intracellular transposition between different replicons and intercellular conjugation and are therefore important agents of ARG ...
Supathep Tansirichaiya +6 more
doaj +1 more source