Results 21 to 30 of about 111,214 (295)

Tetracyclines in veterinary medicine and bacterial resistance to them

open access: yesVeterinární Medicína, 2004
Since their discovery in 1945, tetracyclines have been used extensively in the therapy and prophylaxis of infectious diseases and as growth promoters. These wide applications have led to the equally fast spread of tetracycline resistant strains of gram ...
E. Michalova, Pnovotna, J. Schlegelova
doaj   +1 more source

Waxy allele diversity in waxy maize landraces of Yunnan Province, China

open access: yesJournal of Integrative Agriculture, 2022
Waxy maize is one of the main fresh-eating maize types, and a mutation of the waxy gene causes the waxy character of maize grains. China is rich in waxy maize landraces, and Yunnan and its surrounding areas, are the place of origin and genetic diversity ...
Xiao-yang WU   +6 more
doaj   +1 more source

Implementation of transposon mutagenesis in Bifidobacterium [PDF]

open access: yes, 2019
Random transposon mutagenesis allows for relatively rapid, genome-wide surveys to detect genes involved in functional traits, by performing screens of mutant libraries. This approach has been widely applied to identify genes responsible for activities of
van Sinderen, Douwe   +3 more
core   +1 more source

Progress with schistosome transgenesis

open access: yesMemorias do Instituto Oswaldo Cruz, 2011
Genome sequences for Schistosoma japonicum and Schistosoma mansoni are now available. The schistosome genome encodes ~13,000 protein encoding genes for which the function of only a minority is understood.
Yousef Noori Alrefaei   +3 more
doaj   +1 more source

Transposon-Based Reporter Marking Provides Functional Evidence for Intercellular Bridges in the Male Germline of Rabbits. [PDF]

open access: yes, 2016
The Sleeping Beauty transposon system was established as a robust and efficient method for germline transgenesis in different mammalian species. The generation of transgenic mice, rats, rabbits and swine carrying an identical Venus reporter construct ...
Sabine Klein (196104)   +21 more
core   +1 more source

Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Primordial Germ Cells in vitro or in vivo using the PiggyBac Transposon Vector System

open access: yesThe Journal of Poultry Science, 2015
In the present study, chicken primordial germ cells (PGCs) were transfected with GFP gene in vitro or in vivo using the piggyBac transposon vector system.
Mitsuru Naito   +2 more
doaj   +1 more source

RNA interference is responsible for reduction of transgene expression after Sleeping Beauty transposase mediated somatic integration. [PDF]

open access: yes, 2012
Integrating non-viral vectors based on transposable elements are widely used for genetically engineering mammalian cells in functional genomics and therapeutic gene transfer.
Anja Ehrhardt   +7 more
core   +2 more sources

A Broad Range of Dose Optima Achieve High-level, Long-term Gene Expression After Hydrodynamic Delivery of Sleeping Beauty Transposons Using Hyperactive SB100x Transposase

open access: yesMolecular Therapy: Nucleic Acids, 2016
The Sleeping Beauty (SB) transposon system has been shown to enable long-term gene expression by integrating new sequences into host cell chromosomes. We found that the recently reported SB100x hyperactive transposase conferred a surprisingly high level ...
Kelly M Podetz-Pedersen   +4 more
doaj   +1 more source

Transposon display for active DNA transposons in rice

open access: yesGenes & Genetic Systems, 2007
Transposon display (TD) is a powerful technique to identify the integration site of transposons in gene tagging as a functional genomic tool for elucidating gene function. Although active endogenous DNA transposons have been used extensively for gene tagging in maize, only two active endogenous DNA transposons in rice have been identified, the 0.43-kb ...
Takagi, Kyoko   +4 more
openaire   +3 more sources

Tracing Mobile DNAs: From Molecular to Population Scales

open access: yesFrontiers in Plant Science, 2022
Transposable elements (TEs, transposons) are mobile DNAs that are prevalent in most eukaryotic genomes. In plants, their mobility has vastly contributed to genetic diversity which is essential for adaptive changes and evolution of a species.
Wenwen Fan   +11 more
doaj   +1 more source

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