Results 91 to 100 of about 4,431,538 (308)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

DhSip1, an NRPS-independent siderophore biosynthetic gene, regulates adhesive knob formation and pathogenicity in Dactylellina haptotyla through mediating iron acquisition

Applied and Environmental Microbiology
Iron acquisition plays a crucial role in fungal pathogenicity. Nematode-trapping fungi (NTFs) serve as important biocontrol agents that can develop traps to capture and kill nematodes.
Hong-Mei Lei, Shi-Mei Shen, Ping Xu, Guo-Hong Li, Pei-Ji Zhao   +4 more
doaj   +1 more source

Neutrophil extracellular traps (NETs) - formation and implications.

Acta Biochimica Polonica, 2013
Neutrophils are cells of the immune system which freely circulate in blood vessels and are recruited to the inflammation sites when the human organism responds to microbial infections. One of the mechanisms of neutrophil action is the formation of neutrophil extracellular traps (NETs) The process of NET generation, called netosis, is a specific type of
Zawrotniak, Marcin, Rąpała-Kozik, Maria   +1 more
openaire   +4 more sources

Trap-Seeking or Trap-Digging? Photoinjection of Hydrated Electrons into Aqueous NaCl Solutions

, 2022
It is well-known that when excess electrons are injected into an aqueous solution, they localize and solvate in ∼1 ps. Still debated is whether localization occurs via “trap-digging”, in which the electron carves out a suitable localization site, or by ...
Benjamin J. Schwartz (1235970), Mariah Gomez (13805747), Sanghyun J. Park (12530088), Eric C. Wu (1986352), Wilberth A. Narvaez (11331606)   +4 more
core   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

A mechanosensitive ion channel is required for nematode predation in nematode-trapping fungi

mBio
Mechanosensitive ion channels of small conductance-like channels (MSL) are conserved in cell-walled organisms and enable cellular responses to mechanical stimuli.
Liao Zhang, Jieying Zhu, Jinyuan Kang, Chenyu Liu, Ying Deng, Yani Fan, Xingzhong Liu, Shunxian Wang   +7 more
doaj   +1 more source

Neutrophil Extracellular Trap Formation: Physiology, Pathology, and Pharmacology

Biomolecules, 2019
Neutrophil extracellular traps (NETs), a unique DNA framework decorated with antimicrobial peptides, have been in the scientific limelight for their role in a variety of pathologies ranging from cystic fibrosis to cancer.
Mithunan Ravindran, Meraj A. Khan, Nades Palaniyar   +2 more
doaj   +1 more source

Tomahawk Economy Trap (Model 68)

, 2001
Economy Raccoon/ Feral Cat Transfer Trap (32 x 10 x 12) with 1 x 2 mesh openings (12 gauge) and a rear release door - for less frequent ...
Tomahawk Live Trap Co.
core  

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Protein Tyrosine Phosphatase Regulates Sporulation, Trap Morphogenesis, Stress Responses, and Secondary Metabolism in Arthrobotrys oligospora

Journal of Fungi
Arthrobotrys oligospora is a widely distributed nematode-trapping (NT) fungus that captures nematodes by developing flexible traps. In fungi, protein tyrosine phosphatases (PTPs) are crucial for intracellular signaling, governing processes such as cell ...
Guiqiu Luo, Lirong Zhu, Hui Yuan, Lihua Wei, Yi Chen, Xuemei Li, Jinkui Yang   +6 more
doaj   +1 more source