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Alternatives to Trichoderma reesei in biofuel production
Trends in Biotechnology, 2011Mutant strains of Trichoderma reesei are considered indisputable champions in cellulase production among biomass-degrading fungi. So, it is not surprising that most R&D projects on bioethanol production from lignocellulosics have been based on using T. reesei cellulases. The present review focuses on whether any serious alternatives to T.
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Cellulases of Trichoderma reesei
1988Publisher Summary Trichoderma reesei QM6a and a mutant from this strain produce very effective cellulases. The protein exocellobiohydrolas (CBH 1) is the most abundant of the enzymes from Trichoderma reesei . Trichoderma reesei also produces cellobiase activity.
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In Vivo Footprinting Analysis in Trichoderma reesei
2020The in vivo footprinting method identifies protein-targeted DNA regions under different conditions such as carbon sources. Dimethyl sulfate (DMS) generates methylated purine bases at DNA sites which are not bound by proteins or transcription factors.
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Electrofusion of Trichoderma reesei protoplasts
Letters in Applied Microbiology, 1987Protoplasts of Trichoderma reesei were fused according to the method of Zimmermann. For optimizing the fusion parameters the central composite design was used. Genetic evidence for fusion has been obtained by segregation of the auxotrophic markers in the haploid conidia. The parameters which were optimized were: pulse voltage, pulse duration and number
M. MURKOVIC, W. STEINER, H. ESTERBAUER
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Cellulase production by Trichoderma reesei
European Journal of Applied Microbiology and Biotechnology, 1983A model is proposed for the enzyme production by Trichoderma reesei (QM 9414), which assumes control of the active enzyme transport through the cell membrane as a key parameter for the enzyme activity change in the culture filtrate. In a stirred tank reactor, continuous cultivation of the fungus was carried out in the dilution rate range of D=0.01–0 ...
Anton Ross +2 more
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Promoters and Synthetic Promoters in Trichoderma reesei
Trichoderma reesei holds immense promise for large-scale protein production, rendering it an excellent subject for deeper exploration using genetic engineering methods to achieve a comprehensive grasp of its cellular physiology. Understanding the genetic factors governing its intrinsic regulatory network is crucial, as lacking this knowledge could ...Muhammad, Adnan, Gang, Liu
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Kinetics of protein secretion in Trichoderma reesei
1996In order to obtain knowledge of the protein secretion process in filamentous fungi, and to study the rate-limiting steps in protein production, we have developed methodology for measuring the kinetics of protein production and secretion by metabolic labelling in Trichoderma.
Pakula, Tiina +5 more
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A Putative Lignin Copper Oxidase from Trichoderma reesei
Journal of Fungi (Basel, Switzerland), 2021Emmanuel Bertrand +2 more
exaly
Proteomics studies in Trichoderma reesei
2002Proteome analysis is the simultaneous examination of a large subset of the protein species in a given cell. Traditionally proteomics is performed by two-dimensional gel electrophoresis combined with protein identification by mass spectrometry. We have set up proteomics methodology for the analysis of both extracellular and intracellular proteins of ...
Pakula, Tiina +2 more
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