Results 181 to 190 of about 21,091 (214)
Some of the next articles are maybe not open access.
Crystallization of α-Galactosidase from Trichoderma reesei
Journal of Molecular Biology, 1993An extracellular alpha-galactosidase was isolated from fungus Trichoderma reesei. The purified enzyme had a molecular weight of 54,000 Da and an isoelectric point of 5.25. Crystals of alpha-galactosidase were obtained from a polyethylene glycol 4000 solution by the hanging-drop method. Seeding was used for enlargement of the crystal size.
A M, Golubev, K N, Neustroev
openaire +2 more sources
Sphaeroplast formation and regeneration in Trichoderma reesei
European Journal of Applied Microbiology and Biotechnology, 1981Sphaeroplasts from several genetically marked strains of Trichoderma reesei were readily prepared through enzymatic hydrolysis of mycelial suspensions utilizing Driselase, a commercially available lytic enzyme preparation. Sphaeroplasts were released from the apical tips of hyphae after 90 min exposure to the enzyme and, with longer treatments, from ...
S. K. Picataggio +3 more
openaire +1 more source
Cellulases of Trichoderma reesei
1988Publisher Summary Trichoderma reesei QM6a and a mutant from this strain produce very effective cellulases. The protein exocellobiohydrolas (CBH 1) is the most abundant of the enzymes from Trichoderma reesei . Trichoderma reesei also produces cellobiase activity.
openaire +1 more source
Natural products isolation from Trichoderma reesei
2022The focus of the present work is to develop Trichoderma reesei as a heterologous host for the production of natural products and to explore the biosynthetic potential of this fungus by expressing one of its cryptic PKS-NRPS gene clusters. Developing T.
openaire +1 more source
In Vivo Footprinting Analysis in Trichoderma reesei
2020The in vivo footprinting method identifies protein-targeted DNA regions under different conditions such as carbon sources. Dimethyl sulfate (DMS) generates methylated purine bases at DNA sites which are not bound by proteins or transcription factors.
openaire +2 more sources
การผลิตไซแลนเนสจาก Trichoderma reesei โดยใช้วัสดุเหลือใช้จากการเกษตร
เมื่อนำ Trichoderma reesei Rut C-30 เลี้ยงในอาหารสูตร production medium ที่ pH 5 อุณหภูมิ 30 องศาเซลเซียส โดยใช้วัสดุเหลือใช้จากการเกษตร 4 ชนิด คือรำข้าวสาลี รำข้าวเจ้า เปลือกถั่วลิสง และชานอ้อย เป็นแหล่งคาร์บอน พบว่ารำข้าวสาลีให้แอคติวิตีของไซแลนเนสสูงสุด (23.931 U/ml) และรำข้าวเจ้ามีแอคติวิตีของเซลลูเลสสูงสุด (0.656 U/ml) จากนั้นนำวัสดุเหลือใช ...openaire +1 more source
Proteomics studies in Trichoderma reesei
2002Proteome analysis is the simultaneous examination of a large subset of the protein species in a given cell. Traditionally proteomics is performed by two-dimensional gel electrophoresis combined with protein identification by mass spectrometry. We have set up proteomics methodology for the analysis of both extracellular and intracellular proteins of ...
Pakula, Tiina +2 more
openaire +2 more sources
Promoters and Synthetic Promoters in Trichoderma reesei
Trichoderma reesei holds immense promise for large-scale protein production, rendering it an excellent subject for deeper exploration using genetic engineering methods to achieve a comprehensive grasp of its cellular physiology. Understanding the genetic factors governing its intrinsic regulatory network is crucial, as lacking this knowledge could ...Muhammad, Adnan, Gang, Liu
openaire +2 more sources
Proteome analysis in Trichoderma reesei
2004We have set up methods for intracellular and extracellular proteome analysis in Trichoderma. These include analysis of steady state total protein pattern using 2D gel electrophoresis as well as detection of actively synthesised proteins using metabolic labelling.
Pakula, Tiina +8 more
openaire +1 more source