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Alternatives to Trichoderma reesei in biofuel production

Trends in Biotechnology, 2011
Mutant strains of Trichoderma reesei are considered indisputable champions in cellulase production among biomass-degrading fungi. So, it is not surprising that most R&D projects on bioethanol production from lignocellulosics have been based on using T. reesei cellulases. The present review focuses on whether any serious alternatives to T.
Alexander V Gusakov
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Trichoderma reesei

Trends in Microbiology, 2022
The filamentous fungus Trichoderma reesei (teleomorph: Hypocrea jecorina) grows on rotting plant material in its natural habitat. It is among the most prolific producers of plant cell-wall-degrading enzymes and is frequently used in industry for production of those and other performance proteins.
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Transcriptomics in Trichoderma reesei

2020
Transcriptomics is a powerful technique to study gene expression. The main purpose of transcriptome studies in the filamentous fungus Trichoderma reesei is the analysis of differentially expressed genes as a transcriptional response of the genome to different environmental stimuli or physiological conditions such as sugar availability, nitrogen ...
Amanda C C, Antonieto, Roberto N, Silva
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Molecular Identification of Trichoderma reesei

2020
Fungi comprise one of the most diverse groups of eukaryotes with many cryptic species that are difficult to identify. In this chapter, we detail a protocol for the molecular identification of the most industrially relevant species of Trichoderma-T. reesei.
Mohammad J, Rahimi   +4 more
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On the safety of Trichoderma reesei

Journal of Biotechnology, 1994
Trichoderma reesei has a long history of safe use in industrial-scale enzyme production. Applications of cellulases and xylanases produced by this fungus are found in food, animal feed, pharmaceutical, textile and pulp and paper industries. T. reesei is non-pathogenic for man and it has been shown not to produce fungal toxins or antibiotics under ...
H, Nevalainen, P, Suominen, K, Taimisto
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Electrofusion of Trichoderma reesei protoplasts

Letters in Applied Microbiology, 1987
Protoplasts of Trichoderma reesei were fused according to the method of Zimmermann. For optimizing the fusion parameters the central composite design was used. Genetic evidence for fusion has been obtained by segregation of the auxotrophic markers in the haploid conidia. The parameters which were optimized were: pulse voltage, pulse duration and number
M. MURKOVIC, W. STEINER, H. ESTERBAUER
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Cellulase production by Trichoderma reesei

European Journal of Applied Microbiology and Biotechnology, 1983
A model is proposed for the enzyme production by Trichoderma reesei (QM 9414), which assumes control of the active enzyme transport through the cell membrane as a key parameter for the enzyme activity change in the culture filtrate. In a stirred tank reactor, continuous cultivation of the fungus was carried out in the dilution rate range of D=0.01–0 ...
Anton Ross   +2 more
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Trichoderma reesei cellulases

Trends in Biotechnology, 1983
Abstract Recent advances in the delineation of the biochemical mechanisms of cellulose hydrolysis, strain improvement, molecular cloning and process engineering are bringing T. reesei cellulases closer to being a commercially viable route to cellulose hydrolysis.
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Batch Cultivation of Trichoderma reesei

2020
This chapter explains how to perform a batch cultivation of Trichoderma reesei in bench top bioreactors, exemplarily using wheat straw as sole carbon source, and a selection of recommended, frequently used analyses to monitor the cultivation (intra- and extracellular as well), which are microscopic analysis, sodium hydroxide soluble protein, Bradford ...
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