Linker‐mediated domain separation enhances cold adaptation in cellulases
Abstract Cold‐adapted cellulases have the potential to reduce energy demands in industrial processes by enabling efficient cellulose saccharification at lower temperatures. However, the structural basis of cold adaptation in bidomain cellulases remains poorly understood. Our prior studies on bidomain amylases proposed a “linker spacer effect”—a greater
Robbie Ge+3 more
wiley +1 more source
Genomewide and Enzymatic Analysis Reveals Efficient d-Galacturonic Acid Metabolism in the Basidiomycete Yeast Rhodosporidium toruloides. [PDF]
Biorefining of renewable feedstocks is one of the most promising routes to replace fossil-based products. Since many common fermentation hosts, such as Saccharomyces cerevisiae, are naturally unable to convert many component plant cell wall ...
Arkin, Adam P+10 more
core +1 more source
Genomic and proteomic biases inform metabolic engineering strategies for anaerobic fungi. [PDF]
Anaerobic fungi (Neocallimastigomycota) are emerging non-model hosts for biotechnology due to their wealth of biomass-degrading enzymes, yet tools to engineer these fungi have not yet been established.
Albà+92 more
core +1 more source
Dual Role of MtHAC‐1 in Regulating Cellulase and Xylanase Production in Myceliophthora thermophila
Through a combination of gene deletion, overexpression analyses, and electrophoretic mobility shift assays, we demonstrate that MtHac‐1 acts as a dual‐function regulator of cellulase and xylanase production, directly modulating the expression of major cellulase and xylanase genes, as well as the regulatory gene Mtxyr1, through promoter binding ...
Yapeng Lai+4 more
wiley +1 more source
Fibrolytic activity of four Trichoderma strains grown on agro-industrial residues
The aim of this study was to compare the cellulolytic and xylanolytic activities of two strains of Trichoderma viride, one of Trichoderma reesei and one of Trichoderma harzianum grown on four different substrates. Each substrate contained 20% wheat bran
Vicky García+3 more
doaj
The putative β-glucosidase BGL3I regulates cellulase induction in Trichoderma reesei
Background The filamentous fungus Trichoderma reesei (anamorph of Hypocrea jecorina) displays increased cellulase expression while growing on inducers such as lactose or cellulose. However, the mechanism of cellulase induction in T.
Gen Zou+4 more
doaj +1 more source
Novel genetic tools that enable highly pure protein production in Trichoderma reesei
Trichoderma reesei is an established protein production host with high natural capacity to secrete enzymes. The lack of efficient genome engineering approaches and absence of robust constitutive gene expression systems limits exploitation of this ...
Anssi Rantasalo+5 more
semanticscholar +1 more source
Constitutive hyperproduction of sorbicillinoids in Trichoderma reesei ZC121 [PDF]
In addition to its outstanding cellulase production ability, Trichoderma reesei produces a wide variety of valuable secondary metabolites, the production of which has not received much attention to date. Among them, sorbicillinoids, a large group of hexaketide secondary metabolites derived from polyketides, are drawing a growing interest from ...
Li, Chengcheng+6 more
openaire +5 more sources
Exploring the Critical Environmental Optima and Biotechnological Prospects of Fungal Fruiting Bodies
Mechanisms and strategies for fungal fruiting body development (FBD) are reported. The critical optima for the relevant environmental cues regulating FBD were synthesised. Fruiting body biotechnological applications and recent advances in designed approaches are elucidated.
Amechi S. Nwankwegu+3 more
wiley +1 more source
The research was conducted to study the growth characterization of Trichoderma reesei, the degradability of mannan polysaccharides from Palm Kernel Cake (PKC) with different concentrations of fungi and thickness of the PKC medium, as well as the ...
A Jaelani+3 more
doaj