Results 111 to 120 of about 116,911 (276)

Systematic optimization of human pluripotent stem cells media using Design of Experiments. [PDF]

, 2015
Human pluripotent stem cells (hPSC) are used to study the early stages of human development in vitro and, increasingly due to somatic cell reprogramming, cellular and molecular mechanisms of disease.
Chailangkarn, Thanathom, Marinho, Paulo A, Muotri, Alysson R   +2 more
core   +1 more source

Injectable Dual‐Network Hydrogel System for Osteochondral Repair Combining Immunomodulation, Mechanical Adaptability, and Enhanced Tissue Integration

Advanced Functional Materials, EarlyView.
A UV‐triggered injectable dual‐network hydrogel is reported as the first application of bletilla striata polysaccharide (BSP) in osteochondral repair. By integrating methacrylamide‐modified BSP and nitrobenzaldehyde‐functionalized hyaluronic acid, the system achieves immunomodulation, mechanical reinforcement, and dynamic tissue adhesion, thereby ...
Jiaming Cui, Feifei Zhou, Yuan Luo, Tao Ouyang, Huiwu Zhang, Songmiao Cheng, Qiang Li, Xu Jiang, Yingying Yu, Yicheng Wang, Hong Ming   +10 more
wiley   +1 more source

Padronização da metodologia do RT-PCR utilizado para identificação do mRNA da a-amilase em sementes de milho. [PDF]

, 2019
Objetivou-se padronizar a metodologia do RT-PCR paraidentificar o mRNA do gene de ?-amilase em sementes de milho. Após três dias de germinaçãodas cultivares Saracura-BRS 4154 e CATI-AL34, extraiu-se o RNA total pelo método do tiocianatode guanidina-fenol-
ARAGÃO, C. A., ARAÚJO JÚNIOR, J. P., CAVARIANI, C., DANTAS, B. F., NAKAGAWA, J., RODRIGUES, J. D.   +5 more
core  

Transcriptome analysis of Taenia solium cysticerci using Open reading Frame ESTS (ORESTES) [PDF]

, 2009
Background Human infection by the pork tapeworm Taenia solium affects more than 50 million people worldwide, particularly in underdeveloped and developing countries. Cysticercosis which arises from larval encystation can be life threatening and difficult
Almeida, Carolina R., Bayer-Santos, Ethel, Davila, Alberto M. R., Dias-Neto, Emmanuel, Ferreira, Henrique B., Grisard, Edmundo C., Maia, Antônio A., Ojopi, Elida P. B., Rodrigues, Juliana B., Rotava, Gianinna, Sincero, Thaís C. M., Sperandio, Maísa M., Stoco, Patricia H., Tyler, Kevin M., Wagner, Glauber, Zaha, Arnaldo   +15 more
core   +3 more sources

Self‐Feeding of Engineered Tissues via Controlled Glucose Release Facilitates Survival and Vascularization of Living Implants

Advanced Functional Materials, EarlyView.
ABSTRACT Engineering living matter has great clinical potential to deliver functional replacement organs. However, clinical translation remains hampered by the current inability to maintain viability of clinically relevant‐sized constructs. During the pre‐vascular phase, implants rely on nutrient diffusion for survival, which is insufficient at ...
Melvin Gurian, Iris E. Allijn, Laura Veenendaal, Nicole Bassous, Su Ryon Shin, Jeroen Leijten   +5 more
wiley   +1 more source

Comparación de dos kits de RT-PCR en la detección de ARNm de dos genes endógenos de papa (Solanum tuberosum spp. Andígena)

Revista Colombiana de Biotecnología, 2008
RT-PCR es una técnica en la que usando ARN mensajero como molde, se obtiene complementario o cADN por transcripción inversa, y luego se amplifica uno de los cADN por PCR, mediante el uso de primers específicos.
Milet Zabaleta Vanegas, Mary Luz Yaya-Lancheros, Alejandro Chaparro-Giraldo   +2 more
doaj   +2 more sources

Avaliação da quantidade e qualidade de RNA extraído para diagnóstico de vírus em camarão marinho. [PDF]

, 2011
ENBRAPOA
LEGAT, A. P., OLIVEIRA, J. A., PEREIRA, A. M. L.   +2 more
core  

Tissue Engineered Human Elastic Cartilage From Primary Auricular Chondrocytes for Ear Reconstruction

Advanced Functional Materials, EarlyView.
Despite over three decades of research, no tissue‐engineered solution for auricular reconstruction in microtia patients has reached clinical translation. The key challenge lies in generating functional elastic cartilage ex vivo. Here, we integrate synergistic cell‐biomaterial strategies to engineer auricular grafts with mechanical and histological ...
Philipp Fisch, Sandra Kessler, Simone Ponta, Anna Puiggalí‐Jou, Guoliang Lyu, Killian Flégeau, Anastasiya Martyts, Florian Roth, David Fercher, Filippo M. Rijli, Daniel Simmen, Eva Novoa Olivares, Thomas Linder, Marcy Zenobi‐Wong   +13 more
wiley   +1 more source

Chromatin status and transcription factor binding to gonadotropin promoters in gonadotrope cell lines. [PDF]

, 2017
BackgroundProper expression of key reproductive hormones from gonadotrope cells of the pituitary is required for pubertal onset and reproduction. To further our understanding of the molecular events taking place during embryonic development, leading to ...
Brayman, Melissa J, Hoffmann, Hanne M, Iyer, Anita K, Mellon, Pamela L, Ngo, Cindy, Sunshine, Mary Jean, Xie, Huimin   +6 more
core   +3 more sources

Synergistic All‐in‐One Electroceutical Platform Utilizing a Plasma–Photodynamic Hybrid Approach for Enhanced Wound Healing

Advanced Functional Materials, EarlyView.
A flexible hybrid patch integrating a robust cold atmospheric plasma (CAP) system with a high‐power, ultrathin bio‐OLED was developed to enable synergistic wound healing. This multimodal therapy, combining plasma and photodynamic treatment, enhanced angiogenesis, cell proliferation, and collagen deposition, demonstrating superior in vivo efficacy and ...
Jun‐Yeop Song, Hyun Cheol Bang, Young Hyun Son, Dae‐In Ha, Eun‐Ju Hong, Sun‐Woo Lee, Jeongsook Kim, Doyeong Kim, Sang‐Min Park, Kyu Pil Lee, Hyo‐Jung Kwon, Jeong Hyun Kwon   +11 more
wiley   +1 more source