Results 111 to 120 of about 113,418 (280)

Padronização da metodologia do RT-PCR utilizado para identificação do mRNA da a-amilase em sementes de milho. [PDF]

, 2019
Objetivou-se padronizar a metodologia do RT-PCR paraidentificar o mRNA do gene de ?-amilase em sementes de milho. Após três dias de germinaçãodas cultivares Saracura-BRS 4154 e CATI-AL34, extraiu-se o RNA total pelo método do tiocianatode guanidina-fenol-
ARAGÃO, C. A., ARAÚJO JÚNIOR, J. P., CAVARIANI, C., DANTAS, B. F., NAKAGAWA, J., RODRIGUES, J. D.   +5 more
core  

Systematic optimization of human pluripotent stem cells media using Design of Experiments. [PDF]

, 2015
Human pluripotent stem cells (hPSC) are used to study the early stages of human development in vitro and, increasingly due to somatic cell reprogramming, cellular and molecular mechanisms of disease.
Chailangkarn, Thanathom, Marinho, Paulo A, Muotri, Alysson R   +2 more
core   +1 more source

Enzyme‐Regulated Extended Swelling of Hydrogels for Dehiscence‐Less Tissue Expansions

Advanced Functional Materials, EarlyView.
An interpenetrating hydrogel network with swelling under regulation by enzymatic degradation (INSURED) is fabricated to avoid dehiscence. INSURED remains structurally intact post‐implantation, while HYAL injection enables control over the onset and rate of swelling.
Byung Ik Park, Dong‐yup Lee, Sungsoo Lim, JangHyuk Kim, Min‐Gyu Lee, Chang Seo Park, Hyeonuk Na, Yong Eun Cho, Yun Hyeok Lee, Junsang Doh, Jeong‐Yun Sun   +10 more
wiley   +1 more source

Molecular characterization and expression analysis of three homoeologous Ta14S genes encoding 14-3-3 proteins in wheat (Triticum aestivum L.)

Crop Journal, 2016
The purpose of this study was to characterize Ta14S homoeologs and assess their functions in wheat seed development. The genomic and cDNA sequences of three Ta14S homoeologous genes encoding 14-3-3 proteins were isolated.
Xinguo Wang , Yanli Wang , Ruixia Xiao , Xin Chen , Jiangping Ren   +4 more
doaj   +1 more source

Transcriptome analysis of Taenia solium cysticerci using Open reading Frame ESTS (ORESTES) [PDF]

, 2009
Background Human infection by the pork tapeworm Taenia solium affects more than 50 million people worldwide, particularly in underdeveloped and developing countries. Cysticercosis which arises from larval encystation can be life threatening and difficult
Almeida, Carolina R., Bayer-Santos, Ethel, Davila, Alberto M. R., Dias-Neto, Emmanuel, Ferreira, Henrique B., Grisard, Edmundo C., Maia, Antônio A., Ojopi, Elida P. B., Rodrigues, Juliana B., Rotava, Gianinna, Sincero, Thaís C. M., Sperandio, Maísa M., Stoco, Patricia H., Tyler, Kevin M., Wagner, Glauber, Zaha, Arnaldo   +15 more
core   +3 more sources

DENTA: A Dual Enzymatic Nanoagent for Self‐Activating Tooth Whitening and Biofilm Disruption

Advanced Functional Materials, EarlyView.
The nanoapatite with dual enzymes (DENTA) accumulates in dentinal tubules, reducing hypersensitivity caused by dental nerve exposure and facilitating continuous ROS generation through salivary glucose for effective, long‐term whitening. The dentin structures remain non‐destructive due to the low concentration of ROS, demonstrating excellent cell ...
Junseok Kim, Dai‐Hwan Kim, Priyannth R. Sundharbaabu, Chae Yeon Lee, Jina Bae, Jiyu Hyun, Young‐Ju Jang, Haeni Kim, Min‐Ho Hong, Juewen Liu, Tobias Fey, Suk Ho Bhang, Jun Hyuk Heo, Jung Heon Lee   +13 more
wiley   +1 more source

Avaliação da quantidade e qualidade de RNA extraído para diagnóstico de vírus em camarão marinho. [PDF]

, 2011
ENBRAPOA
LEGAT, A. P., OLIVEIRA, J. A., PEREIRA, A. M. L.   +2 more
core  

3D Differentiation of Bone‐Marrow Derived Mesenchymal Stromal Cells into the Keratocyte Lineage for Corneal Bioprinting

Advanced Healthcare Materials, EarlyView.
This study introduces a novel differentiation protocol for generating corneal stromal keratocyte‐like cells using drop‐on‐demand bioprinting and a collagen‐based hydrogel. The bioprinted constructs maintained a stable dome shape over 21 days while exhibiting upregulated CSK markers postdifferentiation.
Alexandre Taoum, Ronja Friede, Ole Thaden, Oleksandr Rachynskyi, Andrea Frank, Meng Wang, Friederike Dehli, Matthias Fuest, Daniela Duarte Campos   +8 more
wiley   +1 more source

Comparación de dos kits de RT-PCR en la detección de ARNm de dos genes endógenos de papa (Solanum tuberosum spp. Andígena)

Revista Colombiana de Biotecnología, 2008
RT-PCR es una técnica en la que usando ARN mensajero como molde, se obtiene complementario o cADN por transcripción inversa, y luego se amplifica uno de los cADN por PCR, mediante el uso de primers específicos.
Milet Zabaleta Vanegas, Mary Luz Yaya-Lancheros, Alejandro Chaparro-Giraldo   +2 more
doaj   +2 more sources

Plasma Circulating Terminal Differentiation–Induced Non-Coding RNA Serves as a Biomarker in Breast Cancer

International Journal of Hematology-Oncology and Stem Cell Research
Background: Breast cancer is identified as the most common malignancy and cause of cancer-related death worldwide. Compared with healthy controls, this study evaluated the expression level and diagnostic power of lncRNA plasma TINCR in breast cancer ...
Z. Shaghaghi Torkdari, M. Khalaj-Kondori, M. H. Hosseinpour Feizi   +2 more
semanticscholar   +1 more source