Results 41 to 50 of about 113,418 (280)

Comparación de métodos de extracción de RNA para la detección por RT-PCR del Potato yellow vein virus (PYVV) en diferentes órganos de Solanum tuberosum Grupo Phureja

open access: yesRevista Colombiana de Biotecnología, 2013
Título en ingles: Comparison of RNA isolation methods for Potato yellow vein virus (PYVV) detection by RT-PCR in different organs of Solanum tuberosum Group Phureja Título corto: comparación de métodos de extracción de RNA Resumen Potato yellow vein ...
Anngie Katherine Hernández Guzmán   +1 more
doaj   +2 more sources

Gene expression profiling of whole blood: A comparative assessment of RNA-stabilizing collection methods.

open access: yesPLoS ONE, 2019
Peripheral Blood gene expression is widely used in the discovery of biomarkers and development of therapeutics. Recently, a spate of commercial blood collection and preservation systems have been introduced with proprietary variations that may ...
Duncan E Donohue   +8 more
doaj   +1 more source

Canine distemper virus detection in asymptomatic and non vaccinated dogs

open access: yesPesquisa Veterinária Brasileira, 2010
A quantitative real time polymerase chain reaction (PCR) revealed canine distemper virus presence in peripheral blood samples from asymptomatic and non vaccinated dogs.
Helen L. Del Puerto   +5 more
doaj   +1 more source

Differential expression of microRNAs in bovine papillomavirus type 1 transformed equine cells [PDF]

open access: yes, 2016
Bovine papillomavirus (BPV) types 1 and 2 play an important role in the pathogenesis of equine sarcoids (ES), the most common cutaneous tumour affecting horses.
Britton, C.   +4 more
core   +1 more source

Immunocapture RT-qPCR Method for DWV-A Surveillance: Eliminating Hazardous Extraction for Screening Applications

open access: yesApplied Biosciences
Deformed wing virus (DWV) is a major contributor to honey bee colony losses, making effective monitoring essential for apiary management. Traditional DWV detection relies on hazardous RNA extraction followed by RT-qPCR, creating barriers for widespread ...
Krisztina Christmon   +3 more
doaj   +1 more source

Isolation of RNA from Grains of Medicinal Rice “Njavara” using Improved TRIzol Method

open access: yes, 2020
Njavara rice, indigenous to Kerala, is a unique grain plant in the Oryza genus. It is used in the traditional medicine for the treatment of various diseases related to circulatory, respiratory and digestive ailments (Reshmi and Nandini, 2013).
Y. S. Wagh   +7 more
semanticscholar   +1 more source

A Newly Discovered Bordetella Species Carries a Transcriptionally Active CRISPR-Cas with a Small Cas9 Endonuclease [PDF]

open access: yes, 2015
Background Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria.
Dudley, Edward G.   +7 more
core   +2 more sources

A cDNA library of the eutardigrade Hypsibius klebelsbergi Mihelčič, 1959 and analysis of the actin gene

open access: yesJournal of Limnology, 2007
A cDNA library was constructed from the glacier-dwelling eutardigrade Hypsibius klebelsbergi from more than 2000 individuals collected in the Austrian Central Alps. RNA, DNA and proteins were successively isolated by the Trizol®-method.
Hartmut GREVEN   +3 more
doaj   +1 more source

Art of the Kill: Designing and Testing Viral Inactivation Procedures for Highly Pathogenic Negative Sense RNA Viruses

open access: yesPathogens, 2023
The study of highly pathogenic viruses handled under BSL-4 conditions and classified as Select Agents frequently involves the transfer of inactivated materials to lower containment levels for downstream analyses. Adhering to Select Agent and BSL-4 safety
J. Olejnik   +8 more
semanticscholar   +1 more source

Recessive mutation in tetraspanin CD151 causes Kindler syndrome-like epidermolysis bullosa with multi-systemic manifestations including nephropathy [PDF]

open access: yes, 2018
Epidermolysis bullosa (EB) is caused by mutations in as many as 19 distinct genes. We have developed a next-generation sequencing (NGS) panel targeting genes known to be mutated in skin fragility disorders, including tetraspanin CD151 expressed in ...
Abiri, Maryam   +15 more
core   +1 more source

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