Results 41 to 50 of about 111,207 (280)

Isolation of RNA from Grains of Medicinal Rice “Njavara” using Improved TRIzol Method

open access: yes, 2020
Njavara rice, indigenous to Kerala, is a unique grain plant in the Oryza genus. It is used in the traditional medicine for the treatment of various diseases related to circulatory, respiratory and digestive ailments (Reshmi and Nandini, 2013).
Y. S. Wagh   +7 more
semanticscholar   +1 more source

Comparación de métodos de extracción de RNA para la detección por RT-PCR del Potato yellow vein virus (PYVV) en diferentes órganos de Solanum tuberosum Grupo Phureja

open access: yesRevista Colombiana de Biotecnología, 2013
Título en ingles: Comparison of RNA isolation methods for Potato yellow vein virus (PYVV) detection by RT-PCR in different organs of Solanum tuberosum Group Phureja Título corto: comparación de métodos de extracción de RNA Resumen Potato yellow vein ...
Anngie Katherine Hernández Guzmán   +1 more
doaj   +2 more sources

A Newly Discovered Bordetella Species Carries a Transcriptionally Active CRISPR-Cas with a Small Cas9 Endonuclease [PDF]

open access: yes, 2015
Background Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria.
Dudley, Edward G.   +7 more
core   +2 more sources

A cDNA library of the eutardigrade Hypsibius klebelsbergi Mihelčič, 1959 and analysis of the actin gene

open access: yesJournal of Limnology, 2007
A cDNA library was constructed from the glacier-dwelling eutardigrade Hypsibius klebelsbergi from more than 2000 individuals collected in the Austrian Central Alps. RNA, DNA and proteins were successively isolated by the Trizol®-method.
Hartmut GREVEN   +3 more
doaj   +1 more source

Modulation of iron responsive gene expression and enzymatic activities in response to changes of the iron nutritional status in _Cucumis sativus_ L. [PDF]

open access: yes, 2010
Regulation exerted by the iron status of the plant on the iron deficiency responses was investigated in cucumber roots (_Cucumis sativus_ L.) both at the biochemical and molecular level. Absence of iron induced the expression of the CsFRO1, CsIRT1, CsHA1
Gianpiero Vigani   +2 more
core   +2 more sources

Modified CTAB and TRIzol protocols improve RNA extraction from chemically complex Embryophyta

open access: yesApplications in Plant Sciences, 2015
Premise of the study: Here we present a series of protocols for RNA extraction across a diverse array of plants; we focus on woody, aromatic, aquatic, and other chemically complex taxa.
Ingrid E Jordon-Thaden   +3 more
semanticscholar   +1 more source

Art of the Kill: Designing and Testing Viral Inactivation Procedures for Highly Pathogenic Negative Sense RNA Viruses

open access: yesPathogens, 2023
The study of highly pathogenic viruses handled under BSL-4 conditions and classified as Select Agents frequently involves the transfer of inactivated materials to lower containment levels for downstream analyses. Adhering to Select Agent and BSL-4 safety
J. Olejnik   +8 more
semanticscholar   +1 more source

Comparative study of gp130 cytokine effects on corticotroph AtT-20 cells - Redundancy or specificity of neuroimmunoendocrine modulators? [PDF]

open access: yes, 2004
Objective: This comparative in vitro study examined the effects of all known gp130 cytokines on murine corticotroph AtT-20 cell function. Methods: Cytokines were tested at equimolar concentrations from 0.078 to 10 nM.
Auernhammer, Christoph J.   +9 more
core   +1 more source

TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton

open access: yesScientific Reports, 2015
Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton ...
J. Preston Campbell   +8 more
semanticscholar   +1 more source

Double blind control trial of vitamin D fortified milk on the expression of lncRNAs and adiponectin for patients with metabolic syndrome

open access: yesDiabetology & Metabolic Syndrome, 2023
Background Metabolic syndrome (Mets) is a common metabolic disorder in which hypoadiponectinemia is one of the consequences for the body caused by inflammation, and vitamin D may help improve inflammatory symptoms.
Mohammad Rashidmayvan   +10 more
semanticscholar   +1 more source

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