Results 41 to 50 of about 116,911 (276)
Revista Colombiana de Biotecnología, 2013 Título en ingles: Comparison of RNA isolation methods for Potato yellow vein virus (PYVV) detection by RT-PCR in different organs of Solanum tuberosum Group Phureja Título corto: comparación de métodos de extracción de RNA Resumen Potato yellow vein ...
Anngie Katherine Hernández Guzmán +1 more
doaj +2 more sources
PLoS ONE, 2019 Peripheral Blood gene expression is widely used in the discovery of biomarkers and development of therapeutics. Recently, a spate of commercial blood collection and preservation systems have been introduced with proprietary variations that may ...
Duncan E Donohue +8 more
doaj +1 more source
Canine distemper virus detection in asymptomatic and non vaccinated dogs
Pesquisa Veterinária Brasileira, 2010 A quantitative real time polymerase chain reaction (PCR) revealed canine distemper virus presence in peripheral blood samples from asymptomatic and non vaccinated dogs.
Helen L. Del Puerto +5 more
doaj +1 more source
The atypical chemokine receptor ACKR2 is protective against sepsis [PDF]
, 2018 Sepsis is a systemic inflammatory response as a result of uncontrolled infections. Neutrophils are the first cells to reach the primary sites of infection and chemokines play a key role in recruiting neutrophils.
Alves-Filho, José Carlos +15 more
core +1 more source
Applied BiosciencesDeformed wing virus (DWV) is a major contributor to honey bee colony losses, making effective monitoring essential for apiary management. Traditional DWV detection relies on hazardous RNA extraction followed by RT-qPCR, creating barriers for widespread ...
Krisztina Christmon +3 more
doaj +1 more source
Journal of Limnology, 2007 A cDNA library was constructed from the glacier-dwelling eutardigrade Hypsibius klebelsbergi from more than 2000 individuals collected in the Austrian Central Alps. RNA, DNA and proteins were successively isolated by the Trizol®-method.
Hartmut GREVEN +3 more
doaj +1 more source
Avaliação de diferentes métodos para extração de RNA total de folhas e raízes de braquiária. [PDF]
, 2012 A extração de RNA a partir de tecidos específicos consiste no primeiro passo para estudos de expressão gênica e caracterização de transcritos. Plantas, em geral, contêm grande quantidade de compostos fenólicos e/ou polissacarídeos em seus tecidos, o que ...
BITENCOURT, G. de A. +4 more
core
A Newly Discovered Bordetella Species Carries a Transcriptionally Active CRISPR-Cas with a Small Cas9 Endonuclease [PDF]
, 2015 Background Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria.
Dudley, Edward G. +7 more
core +2 more sources
Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10
FEBS Letters, EarlyView.Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang +8 more
wiley +1 more source
Automated RNA Purification directly from TRIzol® on the Hamilton Microlab® STAR™
BioTechniques, 2018 The TRIzol method for RNA extraction has been the gold standard. The powerful protein denaturant effectively stabilizes RNA and inactivates RNases and infectious agents.
doaj +1 more source