Results 51 to 60 of about 116,911 (276)

Comparative study of gp130 cytokine effects on corticotroph AtT-20 cells - Redundancy or specificity of neuroimmunoendocrine modulators? [PDF]

, 2004
Objective: This comparative in vitro study examined the effects of all known gp130 cytokines on murine corticotroph AtT-20 cell function. Methods: Cytokines were tested at equimolar concentrations from 0.078 to 10 nM.
Auernhammer, Christoph J., Cengic, N., Dorn, F., Engelhardt, D., Isele, N. B., Kopp, F. B., Senaldi, G., Spottl, G., Vlotides, G., Weber, M. M.   +9 more
core   +1 more source

Modified CTAB and TRIzol protocols improve RNA extraction from chemically complex Embryophyta

Applications in Plant Sciences, 2015
Premise of the study: Here we present a series of protocols for RNA extraction across a diverse array of plants; we focus on woody, aromatic, aquatic, and other chemically complex taxa.
Ingrid E Jordon-Thaden, Andre S. Chanderbali, Matthew A. Gitzendanner, D. Soltis   +3 more
semanticscholar   +1 more source

Isolation of RNA from Grains of Medicinal Rice “Njavara” using Improved TRIzol Method

, 2020
Njavara rice, indigenous to Kerala, is a unique grain plant in the Oryza genus. It is used in the traditional medicine for the treatment of various diseases related to circulatory, respiratory and digestive ailments (Reshmi and Nandini, 2013).
Y. S. Wagh, M. Viji, K. Soni, P. Jadhav, S. C. Ekatpure, R. Manju, R. Stephen, R. Beena   +7 more
semanticscholar   +1 more source

PARP inhibitors elicit distinct transcriptional programs in homologous recombination competent castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
PARP inhibitors are used to treat a small subset of prostate cancer patients. These studies reveal that PARP1 activity and expression are different between European American and African American prostate cancer tissue samples. Additionally, different PARP inhibitors cause unique and overlapping transcriptional changes, notably, p53 pathway upregulation.
Moriah L. Cunningham, Jasibel Vasquez‐Gonzalez, Samantha M. Barnada, Salome Tchotorlishvili, Latese Jones, Ryan Maguire, Genevieve Lewis, Kinza Rizwan, Jenny Deng, Salma Koachar, Drithi Patel, Hailey Shankle, Tessa Mulders, Namra Ajmal, Charalambos Solomides, Emad S. Alnemri, Teresa F. Alnemri, Ayesha A. Shafi, Leonard G. Gomella, Wm Kevin Kelly, Steven B. McMahon, Matthew J. Schiewer   +21 more
wiley   +1 more source

新たな転写因子の検索法についての研究　BRAK遺伝子の発現上昇に関与する転写因子の検索 [PDF]

, 2016
抗腫瘍性ケモカインBRAK/CXCL14遺伝子の発現上昇に関与する転写因子の検索として、ナラプロテクノロジーのデータマイニングを使用する方法を試みた。マイクロアレイのチップとしてヒトの約1万遺伝子を1枚のスライドグラスに搭載したものを使用し、高密度培養でBRAKと共に発現が上昇したものは1883個、低下が2148個存在した。これらを群分けして発現制御に関わるシグナル伝達経路をKyoto Encyclopedia Genes and Genomes PATHWAY等を参考に検討し、SP1、BACH2 ...
吉田 羊子
core   +1 more source

LINC01116, a hypoxia‐lncRNA marker of pathological lymphangiogenesis and poor prognosis in lung adenocarcinoma

Molecular Oncology, EarlyView.
The LINC01116 long noncoding RNA is induced by hypoxia and associated with poor prognosis and high recurrence rates in two cohorts of lung adenocarcinoma patients. Here, we demonstrate that besides its expression in cancer cells, LINC01116 is markedly expressed in lymphatic endothelial cells of the tumor stroma in which it participates in hypoxia ...
Marine Gautier‐Isola, Rafael Lopes Goncalves, Marin Truchi, Caroline Lacoux, Célia Scribe, Hugo Cadis, Laetitia Guardini, Sophie Bekisz, Marius Ilié, Paul Hofman, Georges Vassaux, Bernard Mari, Roger Rezzonico   +12 more
wiley   +1 more source

Evaluation of TGF-Beta 2 and VEGFα Gene Expression Levels in Epiretinal Membranes and Internal Limiting Membranes in the Course of Retinal Detachments, Proliferative Diabetic Retinopathy, Macular Holes, and Idiopathic Epiretinal Membranes

Journal of Ophthalmology, 2018
Purpose. To evaluate the expression profiles of the VEGFα and TGFβ in the ERMs and ILMs in retinal disorders. Methods. In this nonrandomized prospective study, 75 patients (34 females and 41 males) referred to pars plana vitrectomy (PPV) due to different
Joanna Stafiej, Karolina Kaźmierczak, Katarzyna Linkowska, Paweł Żuchowski, Tomasz Grzybowski, Grażyna Malukiewicz   +5 more
doaj   +1 more source

Comparison of different methods for preparation and characterization of total RNA from cartilage samples to uncover osteoarthritis in vivo

BMC Research Notes, 2010
Background The isolation of intact RNA can be very difficult when tissues are used that contain many RNAses or that are hard to homogenize, e.g. cartilage samples.
Neumann Steffi, Ruettger Anke, Wiederanders Bernd, Huber René   +3 more
doaj   +1 more source

TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis

Reproductive Biology and Endocrinology, 2010
BackgroundAccording to mRNA microarray, proteomics and other studies, biological abnormalities of eutopic endometrium (EM) are involved in the pathogenesis of endometriosis, but the relationship between mRNA and protein expression in EM is not clear.
A. Fassbender, P. Simsa, C. Kyama, E. Waelkens, A. Mihályi, C. Meuleman, O. Gevaert, R. Van de Plas, B. De Moor, T. D’Hooghe   +9 more
semanticscholar   +1 more source

ATF4‐mediated stress response as a therapeutic vulnerability in chordoma

Molecular Oncology, EarlyView.
We screened 5 chordoma cell lines against 100+ inhibitors of epigenetic and metabolic pathways and kinases and identified halofuginone, a tRNA synthetase inhibitor. Mechanistically halofuginone induces an integrated stress response, with eIF2alpha phosphorylation, activation of ATF4 and its target genes CHOP, ASNS, INHBE leading to cell death ...
Lucia Cottone, James Dunford, Eleanor Calcutt, Vicki Gamble, Filiz Senbabaoglu Aksu, Lorena Ligammari, Georgina Wherry, Giorgia Gaeta, John C. Christianson, Adrienne M. Flanagan, Udo Oppermann, Adam P. Cribbs   +11 more
wiley   +1 more source