Results 51 to 60 of about 113,418 (280)

Modified CTAB and TRIzol protocols improve RNA extraction from chemically complex Embryophyta

Applications in Plant Sciences, 2015
Premise of the study: Here we present a series of protocols for RNA extraction across a diverse array of plants; we focus on woody, aromatic, aquatic, and other chemically complex taxa.
Ingrid E Jordon-Thaden, Andre S. Chanderbali, Matthew A. Gitzendanner, D. Soltis   +3 more
semanticscholar   +1 more source

Insight of PBZ mediated drought amelioration in crop plants

Frontiers in Plant Science, 2022
Water scarcity is a significant environmental limitation to plant productivity as drought-induced crop output losses are likely to outnumber losses from all other factors.
Chirag Maheshwari, N. Garg, Muzaffar Hasan, P. V, N. Meena, Ashutosh Kumar Singh, A. Tyagi   +6 more
semanticscholar   +1 more source

Avaliação de diferentes métodos para extração de RNA total de folhas e raízes de braquiária. [PDF]

, 2012
A extração de RNA a partir de tecidos específicos consiste no primeiro passo para estudos de expressão gênica e caracterização de transcritos. Plantas, em geral, contêm grande quantidade de compostos fenólicos e/ou polissacarídeos em seus tecidos, o que ...
BITENCOURT, G. de A., CHIARI, L., LAURA, V. A., MORO, J. R., VALLE, C. B. do   +4 more
core  

Modulation of iron responsive gene expression and enzymatic activities in response to changes of the iron nutritional status in _Cucumis sativus_ L. [PDF]

, 2010
Regulation exerted by the iron status of the plant on the iron deficiency responses was investigated in cucumber roots (_Cucumis sativus_ L.) both at the biochemical and molecular level. Absence of iron induced the expression of the CsFRO1, CsIRT1, CsHA1
Gianpiero Vigani, Graziano Zocchi, Patrizia De Nisi   +2 more
core   +2 more sources

Cancer cell death induced by the NAD antimetabolite Vacor discloses the antitumor potential of SARM1

FEBS Letters, EarlyView.
Vacor, a compound converted into the toxic metabolite Vacor adenine dinucleotide (VAD) by the nicotinamide salvage pathway enzymes NAMPT and NMNAT2, exhibits antitumor activity by inducing rapid and complete NAD depletion. We report that Vacor toxicity is limited to cell lines expressing high levels of SARM1, a NAD glycohydrolase.
Giuseppe Ranieri, Andrea Lapucci, Giuseppe Orsomando, Nadia Raffaelli, Alberto Chiarugi, Daniela Buonvicino   +5 more
wiley   +1 more source

TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton

Scientific Reports, 2015
Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton ...
J. Preston Campbell, P. Mulcrone, S. Masood, M. Karolak, A. Merkel, K. Hebron, A. Zijlstra, J. Sterling, Florent Elefteriou   +8 more
semanticscholar   +1 more source

Comparison of Four Methods of RNA Extraction and cDNA Synthesis from The Venom of Peruvian Snakes of the Genus Bothrops of Clinical Importance

International Journal of Molecular Sciences, 2023
RNA purification and cDNA synthesis represents the starting point for molecular analyses of snake venom proteins-enzymes. Usually, the sacrifice of snakes is necessary for venom gland extraction to identify protein-coding transcripts; however, the venom ...
D. Torrejón, J. Cárdenas, Diana Juárez, Jordano Espinoza, Álex Proleón, Andres Agurto-Arteaga, F. Lazo, Mariana Leguía, F. Urra, E. Sanchez, C. Chávez-Olórtegui, D. Vivas-Ruiz, A. Yarlequé   +12 more
semanticscholar   +1 more source

Targeting EZH2 reverses thyroid cell dedifferentiation and enhances iodide uptake in anaplastic thyroid cancer

FEBS Letters, EarlyView.
Anaplastic thyroid cancer (ATC) lacks iodide uptake ability due to MAPK activation increasing the expression of the histone methyltransferase EZH2, which represses thyroid differentiation genes (TDGs) such as the sodium iodide symporter (NIS). Dual inhibition of MAPK (U0126) and EZH2 (EPZ6438/Tazemetostat) reverses this mechanism, thus restoring TDG ...
Diego Claro de Mello, Marcella Maringolo Cristovão, Guilherme Henrique, Vinicius Gonçalves Rodrigues, Caroline Serrano‐Nascimento, Edna Teruko Kimura, Cesar Seigi Fuziwara   +6 more
wiley   +1 more source

Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis.

Biochemistry and cell biology = Biochimie et biologie cellulaire, 2023
Increasing evidence of sperm RNA's role in fertilization and embryonic development has provided impetus for its isolation and thorough characterization. Sperm are considered tough-to-lyse cells, due to compact condensed DNA in sperm heads.
Saurabh Tiwari, Abdallah M Shahat, Jonathan G. Kastelic, N. Thakor, Jacob Thundathil   +4 more
semanticscholar   +1 more source

Comparative study of gp130 cytokine effects on corticotroph AtT-20 cells - Redundancy or specificity of neuroimmunoendocrine modulators? [PDF]

, 2004
Objective: This comparative in vitro study examined the effects of all known gp130 cytokines on murine corticotroph AtT-20 cell function. Methods: Cytokines were tested at equimolar concentrations from 0.078 to 10 nM.
Auernhammer, Christoph J., Cengic, N., Dorn, F., Engelhardt, D., Isele, N. B., Kopp, F. B., Senaldi, G., Spottl, G., Vlotides, G., Weber, M. M.   +9 more
core   +1 more source