Results 51 to 60 of about 111,207 (280)

Avaliação de diferentes métodos para extração de RNA total de folhas e raízes de braquiária. [PDF]

, 2012
A extração de RNA a partir de tecidos específicos consiste no primeiro passo para estudos de expressão gênica e caracterização de transcritos. Plantas, em geral, contêm grande quantidade de compostos fenólicos e/ou polissacarídeos em seus tecidos, o que ...
BITENCOURT, G. de A., CHIARI, L., LAURA, V. A., MORO, J. R., VALLE, C. B. do   +4 more
core  

The anticancer effect of the HDAC inhibitor belinostat is enhanced by inhibitors of Bcl‐xL or Mcl‐1 in ovarian cancer

Molecular Oncology, EarlyView.
The pan‐HDAC inhibitor belinostat increases the expression of the pro‐apoptotic proteins Bim, Puma, and Noxa and induces apoptosis in ovarian cancer cell lines and patient‐derived tumor organoids when used at high concentrations. Moreover, inhibiting the anti‐apoptotic proteins Bcl‐xL or Mcl‐1 sensitizes these preclinical models to the cytotoxic effect
Cécilia Thomine, Sterenn Guillemot, Louis‐Bastien Weiswald, Romane Florent, Edwige Abeilard, Florence Giffard, Emilie Brotin, Mélanie Briand, Enora Dolivet, Laurent Poulain, Marie Villedieu   +10 more
wiley   +1 more source

Recessive mutation in tetraspanin CD151 causes Kindler syndrome-like epidermolysis bullosa with multi-systemic manifestations including nephropathy [PDF]

, 2018
Epidermolysis bullosa (EB) is caused by mutations in as many as 19 distinct genes. We have developed a next-generation sequencing (NGS) panel targeting genes known to be mutated in skin fragility disorders, including tetraspanin CD151 expressed in ...
Abiri, Maryam, Aristodemou, Sophia, Ertel, Adam, Fortina, Paolo, Kajbafzadeh, Abdol-Mohammad, Kariminejad, Ariana, Liu, Lu, Londin, Eric, Mahmoudi, Hamidreza, McGrath, John A., Saeidian, Amir Hossein, Touati, Andrew, Uitto, Jouni, Vahidnezhad, Hassan, Youssefian, Leila, Zeinali, Sirous   +15 more
core   +1 more source

Comparison of Four Methods of RNA Extraction and cDNA Synthesis from The Venom of Peruvian Snakes of the Genus Bothrops of Clinical Importance

International Journal of Molecular Sciences, 2023
RNA purification and cDNA synthesis represents the starting point for molecular analyses of snake venom proteins-enzymes. Usually, the sacrifice of snakes is necessary for venom gland extraction to identify protein-coding transcripts; however, the venom ...
D. Torrejón, J. Cárdenas, Diana Juárez, Jordano Espinoza, Álex Proleón, Andres Agurto-Arteaga, F. Lazo, Mariana Leguía, F. Urra, E. Sanchez, C. Chávez-Olórtegui, D. Vivas-Ruiz, A. Yarlequé   +12 more
semanticscholar   +1 more source

Loss of primary cilia promotes EphA2‐mediated endothelial‐to‐mesenchymal transition in the ovarian tumor microenvironment

Molecular Oncology, EarlyView.
Loss of primary cilia in endothelial cells promotes EndMT and vascular abnormalities in the ovarian tumor microenvironment through EphA2 activation. Using human samples, in vitro models, and endothelial‐specific Kif3a‐knockout mice, we show that primary cilia loss drives the acquisition of cancer‐associated fibroblast‐like phenotypes, thereby ...
Jin Gu Cho, Yubin Hah, Eunsik Yun, Hye In Ka, Aram Lee, Sora Han, Dawn Lee, Sung Wook Kim, Jong Hoon Park, Byung Su Kwon, Young Yang, Jongmin Kim   +11 more
wiley   +1 more source

Evaluation of TGF-Beta 2 and VEGFα Gene Expression Levels in Epiretinal Membranes and Internal Limiting Membranes in the Course of Retinal Detachments, Proliferative Diabetic Retinopathy, Macular Holes, and Idiopathic Epiretinal Membranes

Journal of Ophthalmology, 2018
Purpose. To evaluate the expression profiles of the VEGFα and TGFβ in the ERMs and ILMs in retinal disorders. Methods. In this nonrandomized prospective study, 75 patients (34 females and 41 males) referred to pars plana vitrectomy (PPV) due to different
Joanna Stafiej, Karolina Kaźmierczak, Katarzyna Linkowska, Paweł Żuchowski, Tomasz Grzybowski, Grażyna Malukiewicz   +5 more
doaj   +1 more source

Comparison of different methods for preparation and characterization of total RNA from cartilage samples to uncover osteoarthritis in vivo

BMC Research Notes, 2010
Background The isolation of intact RNA can be very difficult when tissues are used that contain many RNAses or that are hard to homogenize, e.g. cartilage samples.
Neumann Steffi, Ruettger Anke, Wiederanders Bernd, Huber René   +3 more
doaj   +1 more source

TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis

Reproductive Biology and Endocrinology, 2010
Background According to mRNA microarray, proteomics and other studies, biological abnormalities of eutopic endometrium (EM) are involved in the pathogenesis of endometriosis, but the relationship between mRNA and protein expression in EM is not clear. We
A. Fassbender, P. Simsa, C. Kyama, E. Waelkens, A. Mihályi, C. Meuleman, O. Gevaert, Raf Van de Plas, B. De Moor, T. D’Hooghe   +9 more
semanticscholar   +1 more source

Integrative miRNOMe profiling reveals the miR‐195‐5p–CHEK1 axis and its impact on luminal breast cancer outcomes

Molecular Oncology, EarlyView.
In luminal (ER+) breast carcinoma (BC), miRNA profiling identified miR‐195‐5p as a key regulator of proliferation that targets CHEK1, CDC25A, and CCNE1. High CHEK1 expression correlates with worse relapse‐free survival after chemotherapy, especially in patients with luminal A subtype.
Veronika Boušková, Marie Ehrlichová, Alžběta Spálenková, Ivona Krus, Simona Šůsová, Viktor Hlaváč, Vlasta Němcová, Renata Koževnikovová, Markéta Trnková, David Vrána, Jiří Gatěk, Kateřina Kopečková, Marcela Mrhalová, Soňa Měšťáková, Pavel Souček   +14 more
wiley   +1 more source

新たな転写因子の検索法についての研究　BRAK遺伝子の発現上昇に関与する転写因子の検索 [PDF]

, 2016
抗腫瘍性ケモカインBRAK/CXCL14遺伝子の発現上昇に関与する転写因子の検索として、ナラプロテクノロジーのデータマイニングを使用する方法を試みた。マイクロアレイのチップとしてヒトの約1万遺伝子を1枚のスライドグラスに搭載したものを使用し、高密度培養でBRAKと共に発現が上昇したものは1883個、低下が2148個存在した。これらを群分けして発現制御に関わるシグナル伝達経路をKyoto Encyclopedia Genes and Genomes PATHWAY等を参考に検討し、SP1、BACH2 ...
吉田 羊子
core   +1 more source