Results 61 to 70 of about 27,067 (198)

Characterization of Contractile Proteins from Skeletal Muscle Using Gel-Based Top-Down Proteomics

Proteomes, 2019
The mass spectrometric analysis of skeletal muscle proteins has used both peptide-centric and protein-focused approaches. The term ‘top-down proteomics’ is often used in relation to studying purified proteoforms and their post-translational ...
Paul Dowling, Margit Zweyer, Dieter Swandulla, Kay Ohlendieck   +3 more
doaj   +1 more source

Leiomodin creates a leaky cap at the pointed end of actin-thin filaments.

PLoS Biology, 2020
Improper lengths of actin-thin filaments are associated with altered contractile activity and lethal myopathies. Leiomodin, a member of the tropomodulin family of proteins, is critical in thin filament assembly and maintenance; however, its role is under
Dmitri Tolkatchev, Garry E Smith, Lauren E Schultz, Mert Colpan, Gregory L Helms, John R Cort, Carol C Gregorio, Alla S Kostyukova   +7 more
doaj   +1 more source

A truncation mutant of adenomatous polyposis coli impairs apical cell extrusion through elevated epithelial tissue tension

Cytoskeleton, EarlyView.
Abstract Tissue tension encompasses the mechanical forces exerted on solid tissues within animal bodies, originating from various sources such as cellular contractility, interactions with neighboring cells and the extracellular matrix. Emerging evidence indicates that an imbalance in such forces can influence structural organization, homeostasis, and ...
Wan J. Gan, Rabina Giri, Jakob Begun, Helen E. Abud, Edna C. Hardeman, Peter W. Gunning, Alpha S. Yap, Ivar Noordstra   +7 more
wiley   +1 more source

Structural Effects of Disease-Related Mutations in Actin-Binding Period 3 of Tropomyosin

Molecules, 2021
Tropomyosin (Tpm) is an actin-binding coiled-coil protein. In muscle, it regulates contractions in a troponin/Ca2+-dependent manner and controls the thin filament lengths at the pointed end.
Balaganesh Kuruba, Marta Kaczmarek, Małgorzata Kęsik-Brodacka, Magdalena Fojutowska, Małgorzata Śliwinska, Alla S. Kostyukova, Joanna Moraczewska   +6 more
doaj   +1 more source

The Effect of Tropomyosin Mutations on Actin-Tropomyosin Binding: In Search of Lost Time [PDF]

Biophysical Journal, 2019
The initial binding of tropomyosin onto actin filaments and then its polymerization into continuous cables on the filament surface must be precisely tuned to overall thin-filament structure, function, and performance. Low-affinity interaction of tropomyosin with actin has to be sufficiently strong to localize the tropomyosin on actin, yet not so tight ...
William Lehman, Stuart G. Campbell, Michael J. Rynkiewicz, Jeffrey R. Moore   +3 more
openaire   +3 more sources

Actin Filament Pointed Ends: Assays for Regulation of Assembly and Disassembly by Tropomodulin and Tropomyosin

Cytoskeleton, EarlyView.
ABSTRACT Actin filaments are dynamic polymers whose length depends on regulated monomer association and dissociation at their ends. Actin barbed‐end dynamics are relatively better understood, primarily due to the approximately tenfold faster subunit on/off rates at barbed versus pointed ends.
Sawako Yamashiro, Shashank Shekhar, Stefanie M. Novak, Sudipta Biswas, Carol C. Gregorio, Velia M. Fowler   +5 more
wiley   +1 more source

A Proteomic Study of Atherosclerotic Plaques in Men with Coronary Atherosclerosis

Diagnostics, 2019
Background: To study the changes in protein composition of atherosclerotic plaques at different stages of their development in coronary atherosclerosis using proteomics.
Ekaterina M. Stakhneva, Irina A. Meshcheryakova, Evgeny A. Demidov, Konstantin V. Starostin, Evgeny V. Sadovski, Sergey E. Peltek, Michael I. Voevoda, Alexander M. Chernyavskii, Alexander M. Volkov, Yuliya I. Ragino   +9 more
doaj   +1 more source

Anaphylaxis after Shrimp Intake in a European Pediatric Population: Role of Molecular Diagnostics and Implications for Novel Foods

Children, 2023
(1) Background: Tropomyosin is a major cause of shellfish allergy and anaphylaxis triggered by food. It acts as a pan-allergen, inducing cross-reactivity in insects, dust mites, crustaceans, and mollusks.
Michele Miraglia del Giudice, Giulio Dinardo, Angela Klain, Elisabetta D’Addio, Chiara Lucia Bencivenga, Fabio Decimo, Cristiana Indolfi   +6 more
doaj   +1 more source

Conserved Phosphorylation of the Myosin1e TH1 Domain Impacts Membrane Association and Function in Yeast and Worms

Cytoskeleton, EarlyView.
ABSTRACT Cells have an intrinsic ability to rapidly respond to environmental change to regulate cell cycle progression and membrane organisation, thereby affecting cell growth and division. The actin cytoskeleton is a highly dynamic complex of proteins that can rapidly reorganise to change the growth pattern of a cell.
Holly R. Brooker, Karen Baker, Marina Ezcurra, Philippe P. Laissue, Lin Wang, Michael A. Geeves, Jennifer M. Tullet, Daniel P. Mulvihill   +7 more
wiley   +1 more source

Autoimmunity to Tropomyosin-Specific Peptides Induced by Mycobacterium leprae in Leprosy Patients: Identification of Mimicking Proteins

Frontiers in Immunology, 2018
BackgroundIt has been shown earlier that there is a rise in the levels of autoantibodies and T cell response to cytoskeletal proteins in leprosy. Our group recently demonstrated a rise in both T and B cell responses to keratin and myelin basic protein in
Itu Singh, Asha Ram Yadav, Keshar Kunja Mohanty, Kiran Katoch, Prashant Sharma, Vinay Kumar Pathak, Deepa Bisht, Umesh D. Gupta, Utpal Sengupta   +8 more
doaj   +1 more source