The enthalpy titration of troponin C with calcium
Microcalorimetric titrations have been used to study the binding of Ca2+ to troponin C, the Ca-binding component of troponin. Troponin C was extracted from rabbit skeletal muscle and Ca2+ was added to Ca-free troponin C in the presence of 1 mM Mg2+ at pH 8.83 at 10 degrees C.
Kazuhiro Yamada
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Troponin Variants as Markers of Skeletal Muscle Health and Diseases
Ca2+-regulated contractility is a key determinant of the quality of muscles. The sarcomeric myofilament proteins are essential players in the contraction of striated muscles.
Monica Rasmussen+2 more
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The regulation of troponins I, C and ANP by GATA4 and Nkx2-5 in heart of hibernating thirteen-lined ground squirrels, Ictidomys tridecemlineatus. [PDF]
Hibernation is an adaptive strategy used by various mammals to survive the winter under situations of low ambient temperatures and limited or no food availability.
Bryan E Luu+3 more
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The Solution Structure of a Cardiac Troponin C−Troponin I−Troponin T Complex Shows a Somewhat Compact Troponin C Interacting with an Extended Troponin I−Troponin T Component [PDF]
We have investigated the structure of the cTnC-cTnI-cTnT(198-298) calcium-saturated, ternary cardiac troponin complex by small-angle scattering with contrast variation. Shape restoration was also applied to the scattering information resulting from the deuterated cTnC subunit, the unlabeled cTnI-cTnT(198-298) subunits, and the entire complex.
Paul R. Rosevear+4 more
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The intrinsically disordered C terminus of troponin T binds to troponin C to modulate myocardial force generation. [PDF]
Aberrant regulation of myocardial force production represents an early biomechanical defect associated with sarcomeric cardiomyopathies, but the molecular mechanisms remain poorly defined. Here, we evaluated the pathogenicity of a previously unreported sarcomeric gene variant identified in a pediatric patient with sporadic dilated cardiomyopathy, and ...
Johnston JR+13 more
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Interaction of bepridil with the cardiac troponin C/troponin I complex [PDF]
We have investigated the binding of bepridil to calcium‐saturated cardiac troponin C in a cardiac troponin C/troponin I complex. Nuclear magnetic resonance spectroscopy and [15N,2H]cardiac troponin C permitted the mapping of bepridil‐induced amide proton chemical shifts. A single bepridil‐binding site in the regulatory domain was found with an affinity
Ekram Abusamhadneh+5 more
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Measurement of Calcium Dissociation Rates from Troponin C in Rigor Skeletal Myofibrils
Ca2+ dissociation from the regulatory domain of troponin C may influence the rate of striated muscle relaxation. However, Ca2+ dissociation from troponin C has not been measured within the geometric and stoichiometric constraints of the muscle fiber ...
Sean C Little+4 more
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Effects of temperature and method of heat treatment on myofibrillar proteins of pork [PDF]
During the tests in this paper, meat processing was carried out at different temperatures between the range of 51°C to 100°C. The meat was processed by dry heat (roasting) and wet heat treatments (cooking) in water at atmospheric pressure. After
Vujadinović Dragan+3 more
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The Effect of High-Dose Vitamin C on Biochemical Markers of Myocardial Injury in Coronary Artery Bypass Surgery [PDF]
Objective: To evaluate the effect of high-dose vitamin C on cardiac reperfusion injury and plasma levels of creatine kinase-muscle/brain (CK-MB), troponin I, and lactate dehydrogenase (LDH) in patients undergoing coronary artery bypass grafting (CABG ...
Nafiseh Emadi+3 more
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Elevated serum levels of cardiac troponin and C-reactive protein are associated with all-cause and cardiovascular mortality in patients with end-stage renal disease.
Wei-Jie Li+6 more
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