Results 221 to 230 of about 58,854 (251)

Carnitine in Trypanosoma brucei brucei

Molecular and Biochemical Parasitology, 1982
Abstract Bloodstream and culture forms of Trypanosoma brucei brucei contain high concentrations of intracellular l -carnitine, determined enzymatically (1.46 ± 0.28 and 4.49 ± 0.34 mM , respectively), a large proportion of which is non-esterified.
R A, Klein, J M, Angus, A E, Waterhouse
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Different allele frequencies in Trypanosoma brucei brucei and Trypanosoma brucei gambiense populations

Molecular and Biochemical Parasitology, 1989
Restriction fragment length polymorphism (RFLP) has been analysed in Trypanosoma brucei DNA following hybridization with different DNA probes. This polymorphism seems to be due to allelic variation, and not to variation between sequence duplicates, since the genomic environment of the probed polymorphic fragments is conserved over considerable ...
Paindavoine, Pascale, Bosseler, Françoise, Coquelet, Hélène, Pays, Etienne, Steinert, Maurice   +4 more
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Transketolase in Trypanosoma brucei

Molecular and Biochemical Parasitology, 2011
A single copy gene, encoding a protein highly similar to transketolase from other systems, was identified in the Trypanosoma brucei genome. The gene was expressed in E. coli and the purified protein demonstrated transketolase activity with K(m) values of 0.2mM and 0.8mM respectively for xylulose 5-phosphate and ribose 5-phosphate.
Stoffel, Sabine A., Alibu, Vincent P., Hubert, Jane, Ebikeme, Charles, Portais, Jean-Charles, Bringaud, Frédéric, Schweingruber, M. Ernst, Barrett, Michael P.   +7 more
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Nitric oxide-mediated cytostatic activity on Trypanosoma brucei gambiense and Trypanosoma brucei brucei

Experimental Parasitology, 1992
Macrophages collected from BCG-infected mice or exposed in vitro to interferon-gamma plus lipopolysaccharide developed a cytostatic activity on Trypanosoma brucei gambiense and Trypanosoma brucei brucei. This trypanostatic activity of activated macrophages was inhibited by addition of N-monomethyl-L-arginine, an inhibitor of the L-arginine-nitric oxide
P, Vincendeau, S, Daulouède, B, Veyret, M L, Darde, B, Bouteille, J L, Lemesre   +5 more
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Differential Effects of Trypanosoma brucei gambiense and Trypanosoma brucei brucei on Rat Macrophages

Journal of Parasitology, 2011
Mammalian immune responses to Trypanosoma brucei infection are important to control of the disease. In rats infected with T. brucei gambiense (Wellcome strain; WS) or T. brucei brucei (interleukin-tat 1.4 strain [ILS]), a marked increase in the number of macrophages in the spleen can be observed.
Kazuhiko, Nishimura, Haruna, Nakaya, Hiroshi, Nakagawa, Saburo, Matsuo, Yoshihiro, Ohnishi, Shinji, Yamasaki   +5 more
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Transport of methionine in Trypanosoma brucei brucei

Molecular and Biochemical Parasitology, 2000
African trypanosomes live free in the bloodstream and central nervous system of mammalian hosts and also within the midgut of the tsetse fly vectors which transmit them. The parasite plasma membrane represents the interface between both hosts and parasite, and trypanosomes accumulate many essential metabolites via specific transport processes.
M P, Hasne, M P, Barrett
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Characterisation of pentamidine-resistant Trypanosoma brucei brucei

Molecular and Biochemical Parasitology, 1995
Following selection in vitro by exposure to increasing concentrations of the aromatic diamidine pentamidine, a Trypanosoma brucei brucei clone has been characterised in vivo and in vitro. The resistant clone, designated T.b. brucei S427/118/PR32.6, was found to be less virulent than the parental clone T.b.
Berger, Bradley J., Carter, Nicola S., Fairlamb, Alan H.   +2 more
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Biosynthesis of Trypanothione in Trypanosoma brucei brucei

Biological Chemistry, 2003
Trypanothione [T(SH)2], the major redox mediator in pathogenic trypanosomatids, is synthetized stepwise by two distinct enzymes in Crithidia fasciculata, while in Trypanosoma cruzi a single enzyme catalyzes both steps. A full-length reading frame presumed to encode trypanothione synthetase (TryS) was obtained by PCR using DNA of T.
Marcelo, Comini, Ulrich, Menge, Leopold, Flohé   +2 more
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Kinetics of Methionine Transport and Metabolism by Trypanosoma brucei brucei and Trypanosoma brucei rhodesiense

Archives of Biochemistry and Biophysics, 2000
Methionine is an essential amino acid for both prokaryotic and eukaryotic organisms; however, little is known concerning its utilization in African trypanosomes, protozoa of the Trypanosoma brucei group. This study explored the Michaelis-Menten kinetic constants for transport and pool formation as well as metabolic utilization of methionine by two ...
B, Goldberg, D, Rattendi, D, Lloyd, N, Yarlett, C J, Bacchi   +4 more
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Identification of Trypanosoma evansi, Trypanosoma equiperdum and Trypanosoma brucei brucei using repetitive DNA probes

Veterinary Parasitology, 1994
The phylogenetic relatedness of 15 stocks of Trypanosoma evansi, three stocks of Trypanosoma equiperdum and one stock of Trypanosoma brucei brucei was determined using Southern blot analysis of restriction enzyme digested DNA, probed with two repetitive DNA sequences from T. b. brucei.
Z Q, Zhang, T, Baltz
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