Results 41 to 50 of about 665,350 (364)

Failure to remove bluetongue serotype 8 virus (BTV-8) from in vitro produced and in vivo derived bovine embryos and subsequent transmission of BTV-8 to recipient cows after embryo transfer [PDF]

open access: yes, 2019
The behavior of BTV-8 in cattle is different from most other serotypes not only with regards to clinical signs but certainly with respect to virus transmission (transplacental, contact). Therefore, the possibility of virus transmission by means of embryo
De Clercq, Kris   +6 more
core   +1 more source

Structural analysis of Salmonella enterica effector protein SopD [PDF]

open access: yes, 2004
Salmonella outer protein D (SopD) is a type III secreted virulence effector protein from Salmonella enterica. Full-length SopD and SopD lacking 16 amino acids at the N-terminus (SopDDeltaN) have been expressed as fusions with GST in Escherichia coli ...
Bagby, S   +7 more
core   +1 more source

Calorimetry of Some Trypsin-Trypsin Inhibitor Reactions

open access: yesJournal of Biological Chemistry, 1972
The heats of reaction of trypsin with soybean (Kunitz), ovomucoid, and lima bean inhibitors have been measured at pH 5.0 and 10° and 25°. All the reactions were endothermic, with ΔH ranging from 8.6 Cal per mole for the lima bean inhibitor to 15.3 Cal per mole for the soybean inhibitor. Equilibrium constants for the association were calculated from the
Robert J. Baugh, C.G. Trowbridge
openaire   +3 more sources

Digestion of tropomyosin with trypsin [PDF]

open access: yesFEBS Letters, 1977
Tropomyosin is a rod-like a-helical protein composed of two similar subunits in a coiled-coil configuration. It is situated in the grooves of the F-actin strands and with troponin constitutes the regulatory-complex of vertebrate striated muscle. The sequence of the tropomyosin subunit has been determined by Stone, et al. [ 11.
Witold Drabikowski, A. Gorecka
openaire   +3 more sources

Thermolysin in human cultured keratinocyte isolation

open access: yesBrazilian Journal of Biology
BACKGROUND: When treating extensively burned patients using cultured epidermal sheets, the main problem is the time required for its production. Conventional keratinocyte isolation is usually done using Trypsin. We used a modification of the conventional
A. Gragnani, CS. Sobral, LM. Ferreira
doaj   +1 more source

C‐mannosylation promotes ADAMTS1 activation and secretion in human testicular germ cell tumor NEC8 cells

open access: yesFEBS Letters, EarlyView.
C‐mannosylation is a unique form of protein glycosylation. In this study, we demonstrated that ADAMTS1 is C‐mannosylated at Trp562 and Trp565 in human testicular germ cell tumor NEC8 cells. We found that C‐mannosylation of ADAMTS1 is essential for its secretion, processing, enzymatic activity, and ability to promote vasculogenic mimicry. These findings
Takato Kobayashi   +5 more
wiley   +1 more source

Biochemical characterization of trypsin from Indonesian skipjack tuna (Katsuwonus pelamis) viscera

open access: yesOpen Agriculture
Trypsin production from skipjack tuna (Katsuwonus pelamis) viscera is one significant way to increase the value of fish’s industrial waste. The present work reports the biochemical properties of trypsin from skipjack tuna viscera.
Dali Faiza A.   +3 more
doaj   +1 more source

Potential Activities of Freshwater Exo- and Endo-Acting Extracellular Peptidases in East Tennessee and the Pocono Mountains

open access: yesFrontiers in Microbiology, 2018
Proteins constitute a particularly bioavailable subset of organic carbon and nitrogen in aquatic environments but must be hydrolyzed by extracellular enzymes prior to being metabolized by microorganisms.
Lauren Mullen   +8 more
doaj   +1 more source

C9orf72 ALS‐causing mutations lead to mislocalization and aggregation of nucleoporin Nup107 into stress granules

open access: yesFEBS Letters, EarlyView.
Mutations in the C9orf72 gene represent the most common genetic cause of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Using patient‐derived neurons and C. elegans models, we find that the nucleoporin Nup107 is dysregulated in C9orf72‐associated ALS. Conversely, reducing Nup107 levels mitigates disease‐related changes.
Saygın Bilican   +7 more
wiley   +1 more source

Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja Effect of protease inhibitor on the levels of lipoxygenase in soybean seeds

open access: yesCiência e Agrotecnologia, 2008
As lipoxigenases (LOX) são responsáveis pelo "beany flavor", sabor característico da soja [Glycine max. (L.) Merrill], que é o maior obstáculo ao consumo da soja nos países ocidentais. O inibidor de tripsina Kunitz (KTI) é responsável por 80% da inibição
Josie Gomes de Almeida Barros   +4 more
doaj   +1 more source

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