Results 251 to 260 of about 913,938 (308)

Germ Cell Tumor Cell Culture Techniques

2020
Optimization of cell culture protocol for a given cell line is critical for the proper conduct of in vitro experiments. Because germ cell tumors can be so heterogeneous, optimal culture conditions can vary widely between cell lines. Here, we describe our experience in routine culture and cryopreservation of germ cell tumor cell culture.
John T, Lafin, James F, Amatruda, Aditya, Bagrodia   +2 more
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Immunofluorescent studies of cultured canine tumor cells

International Journal of Cancer, 1972
AbstractThe indirect immunofluorescent (IF) test was used in an immunological survey of 17 sera from tumor‐bearing and normal dogs. These sera were reacted against 33 cultured canine tumor and normal cells. A high percentage (70%) of IF positive reactions were seen in cells derived from sarcomatous tumors while only 4% of the cultures from tumor types ...
C A, Bowles, W, Hagen, J, Ditmore, W T, Kerber, W A, Woods, E M, Jensen   +5 more
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Neurofibromatosis tumor and skin cells in culture

Acta Neuropathologica, 1983
Skin fibroblasts and tumor cells were cultured from four patients with peripheral von Recklinghausen's neurofibromatosis (NF). The cell type enriched in culture from the tumors carried the fibroblastic Thy 1.1. cell surface antigen and produced fibronectin, like fibroblasts from skin of NF-patients or from control persons.
J, Peltonen, H, Aho, U K, Rinne, R, Penttinen   +3 more
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A granulosa cell tumor in tissue culture

American Journal of Obstetrics and Gynecology, 1972
Abstract Normal and neoplastic human granulosa cells were grown in tissue culture and observed directly and by still photography and cinematography. Morphology, growth characteristics, and mitoses were observed, and no differences were detected between the normal and neoplastic cells.
W R, Goldston, G D, Wilbanks, J A, Campbell   +2 more
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Spontaneous cell shedding by tumor cells in monolayer culture

In Vitro Cellular & Developmental Biology, 1986
Sarcoma 180 monolayers spontaneously shed single cells and small multicellular aggregates into the surrounding medium to produce a dual population of floating and substratum-attached cells. Shedding was a motility-associated event that occurred when cells attempted to migrate over one another.
P, Skehan, J E, Thomas, S J, Friedman
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Entosis and Cell Cycle in Tumor Cell Culture

Cell and Tissue Biology, 2019
Entosis is a type of cell cannibalism during which one tumor cell invades another. The fate of the inner cell can vary. It can leave the entotic vacuole, divide within it, or be subjected to lysosome-mediated degradation. The aim of our work was to determine whether MCF7 (p53+) human breast adenocarcinoma cells and A431 (p53-) human epidermoid ...
O. P. Kisurina-Evgenieva, L. A. Khashba, I. A. Mamichev, M. A. Savitskaya, G. E. Onishchenko   +4 more
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Cell culture studies on human nerve sheath tumors

Pathology, 1994
The main controversy about nerve sheath tumors (NSTs) has been their histogenesis. A Schwann cell origin has been proposed by many investigators for both schwannomas and neurofibromas. However Erlandson and Woodruff observed that while schwannomas appeared to be composed predominantly of Schwann cells, neurofibromas consisted of mainly perineurial ...
K, Kharbanda, A K, Dinda, C, Sarkar, A K, Karak, M, Mathur, S, Roy   +5 more
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Tumor-Derived Activated Cells: Culture Conditions and Characterization

2021
This chapter presents results on the expansion and characterization of lymphocyte populations from human tumors. The lymphocytes that grew out of the tumor cell cultures were primarily T cells and were termed tumor-derived activated cells (TDAC). A panel of nine TDAC populations derived from melanoma patients was used to analyze cell surface phenotype ...
J R, Maleckar, C S, Friddell, W M, Lewko, J R, Yannelli, W H, West, R K, Oldham   +5 more
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Adrenocorticotropin-induced unresponsiveness in cultured adrenal tumor cells

Biochimica et Biophysica Acta (BBA) - General Subjects, 1978
Our results demonstrate that adrenocorticotropin (ACTH)-induced refractoriness occurs in cultured adrenal tumor cells. Cells became 85% refractory to ACTH-induced cyclic AMP formation in 20 min and the effect persisted if the hormone remained in the incubation medium.
E, Lifrak, R M, Wishnow
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Establishment of functional human pituitary tumor cell cultures

In Vitro, 1982
Five primary human pituitary tumor cell cultures were initiated from adenoma fragments obtained from patients with prolactin-secreting adenomas and acromegaly. Functional cell cultures were maintained and propagated in monolayer or suspension culture for up to 9 months.
S, Melmed, D, Odenheimer, H E, Carlson, J M, Hershman   +3 more
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