Results 251 to 260 of about 5,932,486 (309)

Germ Cell Tumor Cell Culture Techniques

2020
Optimization of cell culture protocol for a given cell line is critical for the proper conduct of in vitro experiments. Because germ cell tumors can be so heterogeneous, optimal culture conditions can vary widely between cell lines. Here, we describe our experience in routine culture and cryopreservation of germ cell tumor cell culture.
John T, Lafin, James F, Amatruda, Aditya, Bagrodia   +2 more
openaire   +2 more sources

A granulosa cell tumor in tissue culture

American Journal of Obstetrics and Gynecology, 1972
Abstract Normal and neoplastic human granulosa cells were grown in tissue culture and observed directly and by still photography and cinematography. Morphology, growth characteristics, and mitoses were observed, and no differences were detected between the normal and neoplastic cells.
W R, Goldston, G D, Wilbanks, J A, Campbell   +2 more
openaire   +2 more sources

Neurofibromatosis tumor and skin cells in culture

Acta Neuropathologica, 1983
Skin fibroblasts and tumor cells were cultured from four patients with peripheral von Recklinghausen's neurofibromatosis (NF). The cell type enriched in culture from the tumors carried the fibroblastic Thy 1.1. cell surface antigen and produced fibronectin, like fibroblasts from skin of NF-patients or from control persons.
J, Peltonen, H, Aho, U K, Rinne, R, Penttinen   +3 more
openaire   +2 more sources

Spontaneous cell shedding by tumor cells in monolayer culture

In Vitro Cellular & Developmental Biology, 1986
Sarcoma 180 monolayers spontaneously shed single cells and small multicellular aggregates into the surrounding medium to produce a dual population of floating and substratum-attached cells. Shedding was a motility-associated event that occurred when cells attempted to migrate over one another.
P, Skehan, J E, Thomas, S J, Friedman
openaire   +2 more sources

Culturing breast tumor cells in the presence of hormones

Journal of Surgical Oncology, 1974
AbstractA tissue culture technique for culturing human breast tumor cells was utilized to invesitgate the response of these cells to various hormones. Due to the small number of tumors investigated, no definite pattern of response was evident. However, the methods described may provide information which can be of useful clinical importance.
N P, Bishun, J, Mills, R W, Raven, D C, Williams   +3 more
openaire   +2 more sources

Culture and Isolation of Brain Tumor Initiating Cells

Current Protocols in Stem Cell Biology, 2009
AbstractThis unit describes protocols for the culture and isolation of brain tumor initiating cells (BTIC). The cancer stem cell (CSC) hypothesis suggests that tumors are maintained exclusively by a rare fraction of cells that have stem cell properties.
Monika, Lenkiewicz, Na, Li, Sheila K, Singh   +2 more
openaire   +2 more sources

Culture and Isolation of Brain Tumor Initiating Cells

Current Protocols in Stem Cell Biology, 2015
AbstractBrain tumors are typically composed of heterogenous cells that exhibit distinct phenotypic characteristics and proliferative potentials. Only a relatively small fraction of cells in the tumor with stem cell properties, termed brain tumor initiating cells (BTICs), possess an ability to differentiate along multiple lineages, self‐renew, and ...
Parvez, Vora, Chitra, Venugopal, Nicole, McFarlane, Sheila K, Singh   +3 more
openaire   +2 more sources

Transformation of Cells in Human Bone Tumor Cultures

2015
Two cell lines from tumors of 16 patients with osteosarcoma and two cell lines from 5 patients with giant cell tumor of bone showed conversion of cell type after 2 to 13 months in culture. Transformed cells of epithelial like morphology appeared in small focal areas and rapidly overgrew nontransformed cells.
E S, Priori, J R, Wilbur, P T, Allen, J L, East, L, Dmochowski   +4 more
openaire   +2 more sources

Confrontation responses of normal and tumorous cells in culture

Experimental Cell Research, 1970
Abstract 1. 1. A line of normal rat fibroblasts (NRF) was established and a diploid clone was obtained (NRF-C-1). NRF-C-1 cells grew in parallel array as monolayers in tissue culture and did not form tumors on inoculation into isologous newborn rats. 2. 2.
C A, Reznikoff, G O, Gey
openaire   +2 more sources

Antibody transport in cultured tumor cell layers

Journal of Controlled Release, 1998
This review summarizes our recent in vitro studies of the factors affecting the tumor penetration of immunoconjugates. The studies were designed to probe the mechanisms of diffusion and convection, using a cultured layer of mouse melanoma cells as a model tumor cell layer and an antibody to the murine transferrin receptor as a model ligand.
E M, Topp, P A, Kitos, V, Vijaykumar, B S, DeSilva, T L, Hendrickson   +4 more
openaire   +2 more sources