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Culture and Isolation of Brain Tumor Initiating Cells
Current Protocols in Stem Cell Biology, 2015AbstractBrain tumors are typically composed of heterogenous cells that exhibit distinct phenotypic characteristics and proliferative potentials. Only a relatively small fraction of cells in the tumor with stem cell properties, termed brain tumor initiating cells (BTICs), possess an ability to differentiate along multiple lineages, self‐renew, and ...
Parvez, Vora +3 more
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Culture and Isolation of Brain Tumor Initiating Cells
Current Protocols in Stem Cell Biology, 2009AbstractThis unit describes protocols for the culture and isolation of brain tumor initiating cells (BTIC). The cancer stem cell (CSC) hypothesis suggests that tumors are maintained exclusively by a rare fraction of cells that have stem cell properties.
Monika, Lenkiewicz +2 more
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Tumor cell suspension culture on non adhesive substratum
Zeitschrift für Krebsforschung, 1969The authors describe a new method of cell suspension culture. It consists in preventing cell adhesion by using a non-adhesive agar substratum. The non-adhering cells are aggregating in suspension. The morphological study of these cell islands revealed viability and proliferation.
O, Costăchel, L, Fadei, E, Badea
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1995
Publisher Summary This chapter describes generally successful methods for culture initiation of tumor cells from multiple human and animal source tissues. The success rate of 80-90% for the initiation of viable primary cultures with the propensity to maintain the ability to divide and differentiate for at least two to three subcultures (which is 4-10
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Publisher Summary This chapter describes generally successful methods for culture initiation of tumor cells from multiple human and animal source tissues. The success rate of 80-90% for the initiation of viable primary cultures with the propensity to maintain the ability to divide and differentiate for at least two to three subcultures (which is 4-10
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In vitro Culture of Ehrlich Ascites Tumor Cells
Science, 1960The successful culturing and subculturing of ascites tumor cells for 80 odd days are described, as well as the growth pattern of these cells during this period. The cytological appearance of cells after 3 weeks in tissue culture and reinoculation into mice is also presented.
Deschner, E E, Allen, B R
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Transformation of Cells in Human Bone Tumor Cultures
2015Two cell lines from tumors of 16 patients with osteosarcoma and two cell lines from 5 patients with giant cell tumor of bone showed conversion of cell type after 2 to 13 months in culture. Transformed cells of epithelial like morphology appeared in small focal areas and rapidly overgrew nontransformed cells.
E S, Priori +4 more
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111Indium labeling of cultured human tumor cells.
Oncology, 1985At least one microCi of 111indium oxine (111InOx) could be incorporated into 10(6) human tumor cells without cytotoxicity as determined by colony assay or by trypan blue staining. 111In bound to prekilled cells was removed preferentially by washings, and prelabeled cells killed with diphtheria toxin released radioactivity much more rapidly than did ...
A, Lockshin +3 more
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Cultured Kaposi's Sarcoma Tumor Cells Fail to Stimulate T Cell Proliferation
Clinical Immunology and Immunopathology, 1996Prior to the AIDS epidemic, Kaposi's sarcoma (KS) was a rare neoplasm. However, in the context of immunosuppression, cutaneous KS lesions more frequently develop and express various surface molecules recognized by T cells such as intercellular adhesion molecule-1 (ICAM-1; CD54) and HLA-DR.
K E, Foreman +4 more
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Biophysical properties of human astrocytic brain tumor cells in cell culture
Journal of Cellular Physiology, 1980AbstractFor malignant cells cultured from a human astrocytoma, electrophysiological characteristics of the plasma membrane included specific resistivity of 446.82 ± 279.5 ohm·cm2, specific capacitance of 0.758 ± 0.52 microfarads/cm2, time constant 0.318± 0.10 msec.
P M, Black, P L, Kornblith
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GROWTH OF EHRLICH ASCITES TUMOR CELLS IN TISSUE CULTURE
Canadian Journal of Biochemistry and Physiology, 1960Null
Jackson, P W, Giuffre, N, Perlman, D
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