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Germ Cell Tumor Cell Culture Techniques

2020
Optimization of cell culture protocol for a given cell line is critical for the proper conduct of in vitro experiments. Because germ cell tumors can be so heterogeneous, optimal culture conditions can vary widely between cell lines. Here, we describe our experience in routine culture and cryopreservation of germ cell tumor cell culture.
John T, Lafin   +2 more
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Immunofluorescent studies of cultured canine tumor cells

International Journal of Cancer, 1972
AbstractThe indirect immunofluorescent (IF) test was used in an immunological survey of 17 sera from tumor‐bearing and normal dogs. These sera were reacted against 33 cultured canine tumor and normal cells. A high percentage (70%) of IF positive reactions were seen in cells derived from sarcomatous tumors while only 4% of the cultures from tumor types ...
C A, Bowles   +5 more
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Neurofibromatosis tumor and skin cells in culture

Acta Neuropathologica, 1983
Skin fibroblasts and tumor cells were cultured from four patients with peripheral von Recklinghausen's neurofibromatosis (NF). The cell type enriched in culture from the tumors carried the fibroblastic Thy 1.1. cell surface antigen and produced fibronectin, like fibroblasts from skin of NF-patients or from control persons.
J, Peltonen   +3 more
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A granulosa cell tumor in tissue culture

American Journal of Obstetrics and Gynecology, 1972
Abstract Normal and neoplastic human granulosa cells were grown in tissue culture and observed directly and by still photography and cinematography. Morphology, growth characteristics, and mitoses were observed, and no differences were detected between the normal and neoplastic cells.
W R, Goldston   +2 more
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Antibody transport in cultured tumor cell layers

Journal of Controlled Release, 1998
This review summarizes our recent in vitro studies of the factors affecting the tumor penetration of immunoconjugates. The studies were designed to probe the mechanisms of diffusion and convection, using a cultured layer of mouse melanoma cells as a model tumor cell layer and an antibody to the murine transferrin receptor as a model ligand.
E M, Topp   +4 more
openaire   +2 more sources

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