Results 271 to 280 of about 2,710,879 (342)

Spontaneous cell shedding by tumor cells in monolayer culture

In Vitro Cellular & Developmental Biology, 1986
Sarcoma 180 monolayers spontaneously shed single cells and small multicellular aggregates into the surrounding medium to produce a dual population of floating and substratum-attached cells. Shedding was a motility-associated event that occurred when cells attempted to migrate over one another.
P, Skehan, J E, Thomas, S J, Friedman
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Entosis and Cell Cycle in Tumor Cell Culture

Cell and Tissue Biology, 2019
Entosis is a type of cell cannibalism during which one tumor cell invades another. The fate of the inner cell can vary. It can leave the entotic vacuole, divide within it, or be subjected to lysosome-mediated degradation. The aim of our work was to determine whether MCF7 (p53+) human breast adenocarcinoma cells and A431 (p53-) human epidermoid ...
O. P. Kisurina-Evgenieva, L. A. Khashba, I. A. Mamichev, M. A. Savitskaya, G. E. Onishchenko   +4 more
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Cell culture studies on human nerve sheath tumors

Pathology, 1994
The main controversy about nerve sheath tumors (NSTs) has been their histogenesis. A Schwann cell origin has been proposed by many investigators for both schwannomas and neurofibromas. However Erlandson and Woodruff observed that while schwannomas appeared to be composed predominantly of Schwann cells, neurofibromas consisted of mainly perineurial ...
K, Kharbanda, A K, Dinda, C, Sarkar, A K, Karak, M, Mathur, S, Roy   +5 more
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Tumor-Derived Activated Cells: Culture Conditions and Characterization

2021
This chapter presents results on the expansion and characterization of lymphocyte populations from human tumors. The lymphocytes that grew out of the tumor cell cultures were primarily T cells and were termed tumor-derived activated cells (TDAC). A panel of nine TDAC populations derived from melanoma patients was used to analyze cell surface phenotype ...
J R, Maleckar, C S, Friddell, W M, Lewko, J R, Yannelli, W H, West, R K, Oldham   +5 more
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Adrenocorticotropin-induced unresponsiveness in cultured adrenal tumor cells

Biochimica et Biophysica Acta (BBA) - General Subjects, 1978
Our results demonstrate that adrenocorticotropin (ACTH)-induced refractoriness occurs in cultured adrenal tumor cells. Cells became 85% refractory to ACTH-induced cyclic AMP formation in 20 min and the effect persisted if the hormone remained in the incubation medium.
E, Lifrak, R M, Wishnow
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Establishment of functional human pituitary tumor cell cultures

In Vitro, 1982
Five primary human pituitary tumor cell cultures were initiated from adenoma fragments obtained from patients with prolactin-secreting adenomas and acromegaly. Functional cell cultures were maintained and propagated in monolayer or suspension culture for up to 9 months.
S, Melmed, D, Odenheimer, H E, Carlson, J M, Hershman   +3 more
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Culture and Isolation of Brain Tumor Initiating Cells

Current Protocols in Stem Cell Biology, 2015
AbstractBrain tumors are typically composed of heterogenous cells that exhibit distinct phenotypic characteristics and proliferative potentials. Only a relatively small fraction of cells in the tumor with stem cell properties, termed brain tumor initiating cells (BTICs), possess an ability to differentiate along multiple lineages, self‐renew, and ...
Parvez, Vora, Chitra, Venugopal, Nicole, McFarlane, Sheila K, Singh   +3 more
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Culture and Isolation of Brain Tumor Initiating Cells

Current Protocols in Stem Cell Biology, 2009
AbstractThis unit describes protocols for the culture and isolation of brain tumor initiating cells (BTIC). The cancer stem cell (CSC) hypothesis suggests that tumors are maintained exclusively by a rare fraction of cells that have stem cell properties.
Monika, Lenkiewicz, Na, Li, Sheila K, Singh   +2 more
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Tumor cell suspension culture on non adhesive substratum

Zeitschrift für Krebsforschung, 1969
The authors describe a new method of cell suspension culture. It consists in preventing cell adhesion by using a non-adhesive agar substratum. The non-adhering cells are aggregating in suspension. The morphological study of these cell islands revealed viability and proliferation.
O, Costăchel, L, Fadei, E, Badea
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[10] Tumor cell culture

1995
Publisher Summary This chapter describes generally successful methods for culture initiation of tumor cells from multiple human and animal source tissues. The success rate of 80-90% for the initiation of viable primary cultures with the propensity to maintain the ability to divide and differentiate for at least two to three subcultures (which is 4-10
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