A Core35S Promoter of Cauliflower Mosaic Virus Drives More Efficient Replication of Turnip Crinkle Virus [PDF]
The 35S promoter with a duplicated enhancer (frequently referred to as 2X35S) is a strong dicotyledonous plant-specific promoter commonly used in generating transgenic plants to enable high-level expression of genes of interest.
Md Emran Ali, Sumyya Waliullah
doaj +7 more sources
Single-cell mutation rate of turnip crinkle virus (-)-strand replication intermediates. [PDF]
Viruses with single-stranded, positive-sense (+) RNA genomes incur high numbers of errors during replication, thereby creating diversified genome populations from which new, better adapted viral variants can emerge.
Camila Perdoncini Carvalho +6 more
doaj +4 more sources
Turnip crinkle virus‐encoded suppressor of RNA silencing interacts with Arabidopsis SGS3 to enhance virus infection [PDF]
Abstract Most plant viruses encode suppressors of RNA silencing (VSRs) to protect themselves from antiviral RNA silencing in host plants. The capsid protein (CP) of Turnip crinkle virus (TCV) is a well‐characterized VSR, whereas SUPPRESSOR OF GENE SILENCING 3 (SGS3) is an ...
Yan Fu, Xiuchun Zhang
exaly +4 more sources
RNA silencing components mediate resistance signaling against turnip crinkle virus [PDF]
Species-specific immunity is induced when an effector protein from a specific pathogen strain is perceived by a cognate resistance (R) protein in the plant. In Arabidopsis, the R protein HRT, which confers resistance to turnip crinkle virus (TCV), is activated upon recognition of the TCV coat-protein (CP), a potent suppressor of host RNA silencing ...
Shifeng Zhu, Gah-Hyun Lim, Keshun Yu
exaly +5 more sources
Replication-Dependent Biogenesis of Turnip Crinkle Virus Long Noncoding RNAs. [PDF]
Many positive-strand (+) RNA viruses produce long noncoding RNAs (lncRNAs) during the process of cellular infections and mobilize these lncRNAs to counteract antiviral defenses, as well as coordinate the translation of viral proteins. Most viral lncRNAs arise from 5′-to-3′ degradation of longer viral RNAs being stalled at stable secondary structures.
Zhang S +5 more
europepmc +6 more sources
Translation-Independent Roles of RNA Secondary Structures within the Replication Protein Coding Region of Turnip Crinkle Virus [PDF]
RNA secondary structures play diverse roles in positive-sense (+) RNA virus infections, but those located with the replication protein coding sequence can be difficult to investigate.
Rong Sun +3 more
doaj +2 more sources
The generation of Turnip crinkle virus-like particles in plants by the transient expression of wild-type and modified forms of its coat protein [PDF]
Turnip crinkle virus (TCV), a member of the genus carmovirus of the Tombusviridae family, has a genome consisting of a single positive-sense RNA molecule that is encapsidated in an icosahedral particle composed of 180 copies of a single type of coat ...
Keith eSaunders +1 more
doaj +2 more sources
SAG101 forms a ternary complex with EDS1 and PAD4 and is required for resistance signaling against turnip crinkle virus. [PDF]
EDS1, PAD4, and SAG101 are common regulators of plant immunity against many pathogens. EDS1 interacts with both PAD4 and SAG101 but direct interaction between PAD4 and SAG101 has not been detected, leading to the suggestion that the EDS1-PAD4 and EDS1 ...
Shifeng Zhu +6 more
doaj +2 more sources
Membrane insertion and biogenesis of the Turnip crinkle virus p9 movement protein. [PDF]
ABSTRACT Plant viral infection and spread depends on the successful introduction of a virus into a cell of a compatible host, followed by replication and cell-to-cell transport. The movement proteins (MPs) p8 and p9 of Turnip crinkle virus are required for cell-to-cell movement of the virus.
Martínez-Gil L, Johnson AE, Mingarro I.
europepmc +5 more sources
Structural domains within the 3' untranslated region of Turnip crinkle virus. [PDF]
ABSTRACT The genomes of positive-strand RNA viruses undergo conformational shifts that complicate efforts to equate structures with function. We have initiated a detailed analysis of secondary and tertiary elements within the 3′ end of Turnip crinkle virus (TCV) that are required for viral accumulation in ...
McCormack JC +6 more
europepmc +4 more sources

